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Gossypol acetate degrading bacteria S3

A technology of gossypol acetate and degrading bacteria is applied in the field of biological high, which can solve the problems of lack of theoretical basis for process conditions, unclear synergy or antagonism mechanism of compound fermentation strains, etc., so as to control the price of protein resources, alleviate the shortage of protein resources, and shorten the start-up time. effect of time

Inactive Publication Date: 2011-08-17
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

In recent years, the research on fermented cottonseed meal has mainly focused on the selection of virus-free strains and the research on the effect of virus-free, but the synergistic or antagonistic mechanism between the compound fermentation strains is unclear, and the process conditions still lack sufficient theoretical basis

Method used

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  • Gossypol acetate degrading bacteria S3
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Embodiment Construction

[0017] Examples of the present invention are described below.

[0018] The original species of several strains screened in this experiment were activated on the screening medium, and the degradation performance was measured, and they were inoculated on the slant of the test tube for later use. The test tube seed was inoculated in a 1000ml shake flask containing 200ml potato liquid medium, and cultured with constant temperature shaking until the logarithmic phase, and the activated strain was inserted into 20ml potato liquid culture to obtain a first-class strain based on a 100ml Erlenmeyer flask (conditions were 30°C, 200r / min shaker culture 48h), take the primary strain and inoculate 1ml into a 250ml Erlenmeyer flask equipped with 100ml potato liquid medium as the secondary strain (conditions are 30°C, 200r / min shaker culture 48h). The formulation of Gossypol Acetate Sugar-Free Chase Medium is: Gossypol Acetate 3g / L, NaNO32g / L, KCl 0.5g / L, FeSO40.01g / L, MgSO4 7H2O 0.5g / L, K2H...

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Abstract

The invention provides gossypol acetate degrading bacteria S3 capable of degrading free gossypol. The gossypol acetate degrading bacteria S3 are identified to be the Candidaoleophila. The Candidaoleophila has the main biological characteristic that the Candidaoleophila is olive candida, white, circular, propagated in a budding way, white pseudohypha, and regularity in edge; the Candidaoleophila can grow in a potato agar culture medium and can grow in a Czapek culture medium to ferment sucrose; and the Candidaoleophila can grow by taking 0.3% gossypol acetate as an only carbon source. The degrading ratio of the predominant bacteria S3 to gossypol is 60.44%, and the CP (cellulose propionate) improving ratio is 26.02%. Furthermore, a bacterial strain is very good in stability, so that cotton seed meal achieves the use standard, and the use ratio of the cotton seed meal is enhanced. The gossypol acetate degrading bacteria S3 have the important meanings for remitting the shortage of protein resources, reducing the soybean import and the cotton seed meal low-price export to Japan, controlling the continuous rising of the protein resources price, and enhancing the national power.

Description

1. Technical field [0001] The invention relates to a high-concentration gossypol-tolerant bacterial strain, which belongs to the high-tech field of biology, uses microorganisms to degrade free gossypol, and is suitable for the biological treatment of modern cottonseed meal with high-concentration gossypol content. 2. Technical Background [0002] Cottonseed meal is an important plant protein feed resource, but due to the existence of gossypol, low and unbalanced amino acid content and high crude fiber content in cottonseed meal, it has become the main factor limiting its wide use in aquaculture. A certain amount of gossypol (FG) in the diet can have negative effects on animal growth, reproduction, gastrointestinal tract and body tissues and organs. "Sealing" makes FG lose its toxicity, and it is difficult to be digested and absorbed by animals after it is combined. However, it has been reported that the combination of gossypol can release FG during animal digestion, resultin...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12R1/72
Inventor 高峰夏新成林海晶江芸周光宏
Owner NANJING AGRICULTURAL UNIVERSITY
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