Method for preparing beta-mannanase by using natural Bacillus subtilis

Abstract

The invention provides a method for preparing beta-mannanase by using natural Bacillus subtilis, which comprises: culturing Bacillus subtilis HSO121 by using culture medium containing refined rivier giantarum rhizome powder and a composite nitrogen source, centrifuging the obtained culture liquid to remove thallus cells and thus obtaining coarse beta-mannanase liquid, wherein the mass concentration of refined rivier giantarum rhizome powder in the culture medium is 2.5 to 5.0 percent; and the composite nitrogen source comprises KNO3 at a concentration of 80 to 140mmol / L and peptone at a concentration of 0.1 to 1 weight percent. In the invention, the beta-mannanase is prepared by optimizing the formula of the culture medium and by using the natural Bacillus subtilis HSO121, so the beta-mannanase yield of the fermentation of the Bacillus subtilis HSO121 is improved and a good technical effect is produced.

Description

technical field

[0001] The invention relates to the technology of producing enzymes by microorganisms, in particular to a preparation method of β-mannanase. Background technique

[0002] Mannan is rich in sources, and different types of mannan exist in konjac flour, guar gum, locust bean gum, etc. Backbone mannan degrading enzymes include β-mannanase, β-glucosidase, β-mannosidase. Among them, the most important is β-mannanase. β-mannanase is a kind of endohydrolase that can hydrolyze mannan oligosaccharides and mannan polysaccharides containing β-1,4-D-mannosidic bonds. It belongs to hemicellulase and is widely found in animals and plants. and microorganisms. Among them, microorganisms are the main source of β-mannanase, which has the advantages of high activity and stable source.

[0003] With the development of natural hemicellulose resources in recent years, the research and development of β-mannanase has entered a new climax, and has been widely used in food, medicin...