Method for preparing beta-mannanase by using natural Bacillus subtilis
The technology of Bacillus subtilis and mannanase is applied in the field of preparation of β-mannanase, which can solve the problems of limitation, unstable performance of genetically engineered bacteria, ecological safety, etc. The effect of increasing the amount of enzymes produced by fermentation
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[0019] Example 1
[0020] Based on medium: konjac powder 2.5~5.0% (wt%), peptone 0.1% (wt%), KH 2 PO 4 0.1% (wt%), MgSO 4 0.01% (wt%) is the culture medium, the Bacillus subtilis HSO121 is cultured at pH: 7-7.2, 37°C, 120r / min, after 32 hours of culture, the crude enzyme solution is obtained after centrifugation to remove the cells. The maximum enzyme activity measured when reacting with the enzyme solution at 55°C for 10 min was 13.16 U / mL.
[0021] Konjac powder (wt%)
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[0022] Example 2
[0023] With medium: konjac flour 3.5% (wt%), KNO 3 80~140mmol / L, peptone 0.1% (wt%), KH 2 PO 4 0.1% (wt%), MgSO 4 0.01% (wt%) is the culture medium, the Bacillus subtilis HSO121 is cultured at pH: 7-7.2, 37°C, 120r / min, after 32 hours of culture, the crude enzyme solution is obtained after centrifugation to remove the cells. The maximum enzyme activity measured when reacting with the enzyme solution at 55°C for 10 minutes was 9.68 U / mL.
[0024] KNO 3 mmol / L
Example Embodiment
[0025] Example 3
[0026] With medium: konjac flour 3.5% (wt%), KNO 3 100mmol / L, peptone 0.1%~1.0% (wt%), KH 2 PO 4 0.1% (wt%), MgSO 4 0.01% (wt%) is the culture medium, the Bacillus subtilis HSO121 is cultured at pH: 7-7.2, 37°C, 120r / min, after 32 hours of culture, the crude enzyme solution is obtained after centrifugation to remove the cells. The maximum enzyme activity measured when reacting with the enzyme solution at 55°C for 10 minutes was 14.31 U / mL.
[0027] Peptone (wt%)
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