High-efficiency expression vector of xylanase in bacillus subtilis and construction method thereof

Abstract

The invention discloses a high-efficiency expression vector of xylanase in bacillus subtilis and a construction method thereof. The expression vector comprises escherichia coli replicor ColE1, bacillus subtilis replicor B. sub. rep, selection marker and a promoter, wherein the downstream of the promoter is provided with a signal peptide sequence and a xylanase (XynA) gene sequence; and escherichia coli-bacillus subtilis is taken as a shuttle vector, and the high-efficiency expression of the xylanase can be realized by screening signal peptide with strong expression for the xylanase.

Description

technical field [0001] The invention relates to a high-efficiency expression vector of xylanase in Bacillus subtilis and a construction method thereof. Background technique [0002] According to the statistics of the World Food and Agriculture Organization, as of 2010, the number of hungry people in the world has exceeded 1 billion. With the growth of population and the development of animal husbandry, the contradiction between humans and animals competing for food has become increasingly prominent. At the same time, the rapid development of animal husbandry has also brought serious environmental problems to the world. question. In this case, it is particularly important to find and develop new feed resources and improve the utilization efficiency of existing feed resources. Cellulose and hemicellulose are renewable natural resources that are abundant in nature. Therefore, the full development of cellulose and hemicellulose can effectively expand the development scope of m...

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Problems solved by technology

However, Escherichia coli also has its own shortcomings: its expressed protein is easy to form inclusion bodies, which is not conducive to downstream purification and processing. At the same time, the expressed protein of xylanase cannot be secreted outside the cell. come to trouble

As far as the current discovery is concerned, there are 148 signal peptides in the Sec pathway, and 27 signal peptides in the Tat pathway. A large number of signal peptides have different guiding secretion efficiencies for xylanase. In the process of guiding the expression of xylanase, It is difficult to screen out a signal peptide that efficiently guides the expression of xylanase, and it consumes a lot of manpower and material resources to construct a high-efficiency expression vector of xylanase in Bacillus subtilis

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