Method for preparing flavor for elsholtzia kachinensis prain tobacco through multi-strain composite fermentation
A compound fermentation and tobacco flavoring technology is applied in the preparation of tobacco flavorings and in the field of producing characteristic tobacco flavorings, achieving the effects of unique application cost, simple equipment, and enhanced plumpness and richness.
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Embodiment 1
[0026] The method for preparing the spice for S. serrata by compound fermentation of multiple strains adopts extraction and refining after two fermentations to prepare the spice for S. serrata;
[0027] The steps of the first fermentation method are as follows:
[0028] ①Bacterial activation: Inoculate the cherry seed yeast obtained by conventional microbial isolation and purification methods on the PDA medium prepared in advance, and cultivate it in a constant temperature environment of 20℃~35℃ for 12~36 hours to obtain activated bacteria Species
[0029] ②Preparation of seed solution: Take the activated bacteria, inoculate it in PDA liquid medium, sterilize the first-level seed culture solution at 110℃~125℃ for 20~40 minutes, and place it at 20℃~35℃, 150 revolutions. Cultivate in bed for 1 to 2 days to obtain first-level seed liquid;
[0030] ③Raw material treatment: select fresh raw material of serrata sylvestris from Yunnan, beaten it by weight ratio of serrata serrata raw materi...
Embodiment 2
[0041] First carry out the first fermentation as follows:
[0042] ①Strain activation: inoculate the watermelon yeast obtained by conventional microbial isolation and purification methods on the PDA medium prepared in advance, and cultivate it in a constant temperature environment of 20°C for 36 hours to obtain an activated strain;
[0043] ②Seed solution preparation: Take the activated strains, inoculate them in PDA liquid medium, sterilize the first-level seed culture solution at about 120°C for 30 minutes, and place it on a shaker at 30°C and 150 revolutions for 1-2 days. Seed liquid
[0044] ③Raw material processing: select fresh Guangxi serrata raw material, and after beating the serrata serrata raw material: water=1: 2 (W / W) by weight, add it according to the serrata serrata raw material: water=1: 8 (W / W) Water, heat reflux and extract for 2 hours at 100℃~120℃, then filter the extract, divide the filtrate into 100~300ml / bottle, and then package and sterilize at 110℃~125℃, 20~6...
Embodiment 3
[0055] The steps of the first fermentation method are as follows:
[0056] ①Strain activation: Inoculate the goat milk fruit yeast obtained by conventional microorganism isolation and purification methods on the PDA medium prepared in advance, and cultivate it in a constant temperature environment of 35°C for 12 hours to obtain an activated strain;
[0057] ②Seed solution preparation: Take the activated strains, inoculate it in PDA liquid medium, sterilize the first-level seed culture solution at 125°C for 20 minutes, and place it on a shaker at 20°C and 150 rpm for 2 days to obtain the first-level seed solution ;
[0058] ③Raw material processing: select fresh raw material of serrata serrata, after beating the raw material of serrata serrata: ethanol = 1:3 (W / W), and add ethanol according to the raw material of serrata serrata: water = 1:5 (W / W). Heat reflux extraction at 180°C for 1 hour, filter, divide the filtrate, and pack and sterilize at 110°C to 125°C for 20 to 60 minutes to...
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