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Application of waste compost microbial agent to improving salt resistance of lawn grass

A technology for microbial inoculants and compound microbial inoculants, which can be used in applications, fertilizer mixtures, botanical equipment and methods, etc., and can solve problems such as complexity, symbiosis of composting bacteria, and ambiguity of mutual influence relationships.

Inactive Publication Date: 2011-09-07
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the research process of compound microbial agents, the identification and classification of microbial species in compost, the symbiosis and mutual influence relationship between compost strains are ambiguous and complex, and the application of compost microbial agents to plants under normal conditions In terms of the effect on growth, there are few related studies reported
However, the identification and classification of microbial species in compost, the symbiosis and mutual influence relationship between compost strains are fuzzy and complex, and the extraction, screening and preparation of compost compound beneficial microbial agents from municipal solid waste composting are effective for salt stress. Studies on the influence of turf plant growth and physiological characteristics have not been reported

Method used

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  • Application of waste compost microbial agent to improving salt resistance of lawn grass
  • Application of waste compost microbial agent to improving salt resistance of lawn grass
  • Application of waste compost microbial agent to improving salt resistance of lawn grass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Screening of bacterial strains

[0019] ① Enrichment: Weigh 10 g of the collected compost sample into a sterile Erlenmeyer flask, add 100 ml of sterile water and glass beads and shake evenly, then take 10 ml of the suspension in a triangle flask containing 100 ml of enrichment medium. In the bottle, shake culture at 28°C and 220 r / min for 3-5 days. ②Preliminary screening: Dilute the enriched culture solution in a concentration gradient, spread it on the separation and screening medium, incubate at a constant temperature upside down, observe the size of the transparent circle, and select the colony with a large ratio of transparent circle / colony diameter for recombination. screen. ③Growth curve drawing: Inoculate the Bacillus subtilis and yeast obtained from the primary screening into the corresponding shake flask culture medium, culture them at 37°C and 28°C, and 220 r / min respectively, and use wavelengths of 600 nm and The OD value at 560 nm was plotted on the ord...

Embodiment 2

[0023] Application of compound microbial agent

[0024] A single-factor randomized block experimental design was adopted, and the limiting factors were: ① Salt stress, three levels of salt stress treatment; mild (0.3%NaCl): T 1 , Moderate (0.6%NaCl): T 2 and severe (0.9%NaCl) saline-alkali soil: T 3 . ② Apply different concentrations of compound microbial agents, and set the treatment method under this salt stress condition: treatment 1 is the control, without inoculation of the agent (CK); treatment 2 is added with a 100-fold dilution of the agent (CM 1 ); Treatment 3 added 200-fold dilution of the bacterial agent (CM 2 ); Treatment 4 was added with 300-fold diluted bacterial agent (CM 3 ).

Embodiment 3

[0025] Embodiment 3 lawn establishment

[0026] The perennial ryegrass ( Lolium perenne L.) and tall fescue ( Festuca arundinaceaL.) is the test material. The seeds of the tested turfgrass plants were plump and uniform in size. The soil used is the garden soil of Tianjin Normal University. Its physical and chemical properties are: pH 7.44, saturated water content 0.58 ml / g, organic matter 4.68%, total nitrogen 0.21%, available phosphorus 22.03 mg / kg, and total potassium 45.61 g / kg. After collection, remove plant residues, gravel and other sundries, pass through a 2 mm sieve, and sterilize at 121 °C for 30 min for later use. The method of potting was adopted in the experiment, 200 g of sterilized soil was placed in each culture container, and 0.4 g of lawn seeds were sown on it. Provide uniform and quantitative water every day to maintain a good water condition in the culture medium, and change the position of each culture container frequently to ensure consistent light. ...

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Abstract

The invention discloses an application of a city domestic waste compost microbial agent to improving salt resistance of lawn grass. The composite microbial agent consists of three bacteria comprising bacillus subtilis, actinomycetes and saccharomycetes according to a volume ratio at 1:1:1, and the composite microbial agent is 100-200 folds diluted; the salt resistance refers to 0.3%-0.9% NaCl saline-alkali soils. The experimental result shows that: after two lawn plants are inoculated with the city domestic waste compost microbial agent, the maximum stress capacity for resisting salt stress can reach 0.9% of salt stress, so that the 100-200 folds of diluted composite microbial agent prepared by the invention obviously promotes the relieving of lawn plant salt stress.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to the preparation of a high-efficiency composting compound bacterial agent by using beneficial bacteria extracted from urban domestic garbage composting as raw materials. More specifically, it is the application of compost compound microbial agent in improving the salt resistance of lawn plants. Background technique [0002] Composting is a biochemical process that uses microorganisms (bacteria, actinomycetes, fungi, etc.) widely present in nature or commercial strains to controlly promote the transformation of biodegradable organic matter into stable humus (humic substance, HS). . This process is also part of the ecological process on the earth's surface, which participates in the cycle of matter and energy on the earth's surface. [0003] Under artificial control conditions, the organic matter in solid waste can be converted into organic fertilizer (compost) thro...

Claims

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Application Information

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IPC IPC(8): A01G1/00C05G3/04C05G3/80
Inventor 多立安赵树兰王晶晶
Owner TIANJIN NORMAL UNIVERSITY
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