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Method for preparing oligomeric sialic acid

A polysialic acid and solution technology, applied in the field of bioengineering, can solve the problems of difficult control of reaction system temperature, difficult control, serious pollution, etc., and achieve the effects of convenient hydrolysis reaction, energy saving and manpower saving

Inactive Publication Date: 2011-09-07
JIANGSU RUIGUANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acid hydrolysis, such as using sulfuric acid and hydrochloric acid to hydrolyze polysialic acid, these methods have serious pollution and are not easy to control the end point of the reaction
The microwave method is not easy to control the temperature of the reaction system

Method used

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  • Method for preparing oligomeric sialic acid
  • Method for preparing oligomeric sialic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Hydrolysis of polysialic acid

[0023] Take 100g of polymerized sialic acid, dissolve it in 1L of 50mM NaOAc solution (the pH of the solution is adjusted to pH 4.7 with anhydrous acetic acid), and hydrolyze at 65°C for 2 hours.

Embodiment 2

[0024] Embodiment 2: the hydrolysis of polysialic acid

[0025] Take 100g of polymerized sialic acid, dissolve it in 0.5L of 50mM NaOAc solution (the pH of the solution is adjusted to pH 4.7 with anhydrous acetic acid), and hydrolyze at 60°C for 3 hours.

Embodiment 3

[0026] Embodiment 3: Extraction of oligosialic acid

[0027] A membrane with a molecular weight cut-off of 8000 is selected for separation by a hollow roll membrane separation method. The operating pressure is 0.5MPa. The reaction mixture is pushed through the membrane with 2.5-5L pH4.7 50mM NaOAc solution, and the ultrafiltration time is 25 minutes. The retentate continues to be used for hydrolysis. The hydrolysis process is operated according to hydrolysis at 60°C for 30 minutes and ultrafiltration for 25 minutes. Ultrafiltration is carried out according to the volume ratio of 1:5-10. Concentration of the permeate is carried out with a desalination membrane with a molecular weight cut off of 400, the operating pressure is 0.5 MPa, and the ultrafiltration time is 30 minutes. The intercepted concentrated solution is subjected to freeze-drying to obtain an oligomeric sialic acid product with a degree of polymerization of 2-10. The product yield rate is above 80%.

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Abstract

The invention relates to a method for preparing oligomeric sialic acid, belonging to the technical field of biological engineering. The method comprises the following steps: hydrolyzing alpha2-8 connected polysialic acid under a mild condition, separating by using an ultrafiltration membrane, collecting the oligomeric sialic acid with the degree of polymerization of 2-10, desalting, concentratingby using a concentration membrane separation system, freezing and drying to obtain the product which is the oligomeric sialic acid. The oligomeric sialic acid can be used as a functional nutritional additive and can be applied to the technical fields of cosmetics, medicaments and other biological medicaments.

Description

technical field [0001] The invention relates to a preparation method of oligosialic acid, which belongs to the technical field of bioengineering. Background technique [0002] Sialic acid (SA), known scientifically as "N-acetylneuraminic acid," is a naturally occurring carbohydrate. It was originally isolated from submandibular gland mucin, hence the name. In the human body, the content of sialic acid in the brain is the highest, and the content of sialic acid in the gray matter of the brain is 15 times that of internal organs such as the liver and lungs. [0003] Polysialic acid (PSA) is a homopolymer of sialic acid (NeuAc, NANA) monomers linked by α-2,8 or α-2,9 glycosidic bonds. Components of glycoproteins and exopolysaccharide components of a few bacteria (Neisseria meningitidis, E. coli strain K1, etc.). α-2,8-linked polysialic acid is the only non-immunogenic (does not elicit T cell or antibody responses in mammalian individuals) among bacterial polysaccharides, whi...

Claims

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Application Information

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IPC IPC(8): C07H5/06C07H1/00C08B37/00
Inventor 朱莉
Owner JIANGSU RUIGUANG BIOTECH
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