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Deminamicin and preparation method and application thereof

A macrolide, endophytic actinomycete technology, applied in the field of microbial engineering, can solve the problems such as no research report on the secondary metabolites of termite endophytic actinomycetes, and achieves short cycle, low cost and simple process Effect

Inactive Publication Date: 2011-09-07
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there has been no research report on the secondary metabolites of termite endophytic actinomycetes

Method used

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  • Deminamicin and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation and purification of termite endophytic actinomycete BY-4

[0028] The actinomycete used in the present invention is the black-winged soil termite captured from the southern foot of Zijin Mountain in Nanjing in September 2005 Odontotermes formosanus An endophytic actinomycete isolated from the intestinal tract of (Shiraki), the strain number is BY-4, and it is now preserved in the China Center for Type Culture Collection (Address: Wuhan Luojia Mountain, Wuhan University), and the preservation number is CCTCC M 2010366, the deposit date is December 27, 2010.

[0029] Under sterile conditions, disinfect the surface of termites in 70% alcohol for 2 minutes, cut off the head, take the abdomen, rinse the abdomen with sterile water for 3 times, add a small amount of sterile water to grind in a sterile mortar, and use sterile Bacterial water was diluted to a gradient of 10 -1 , 10 -2 , 10 -3 , 10 -4 , respectively take 0.2 ml of each gradient dilution...

Embodiment 2

[0030] Embodiment 2: the liquid fermentation of termite endophytic actinomycetes BY-4

[0031] Activate termite endophytic actinomycete BY-4, inoculate fresh bacterium blocks into 1000 mL Erlenmeyer flasks, each bottle contains 400 mL of Gao’s No. 1 medium, inoculate 5-6 bottles on a shaker, at Cultivate at rpm and 28°C for 5 days as seed liquid, then inoculate 20 mL of seed liquid into a 1000 mL Erlenmeyer flask containing 400 mL of Gao's No. 1 medium, and ferment at 140 rpm and 28°C for 14 days .

Embodiment 3

[0032] Embodiment 3: Extraction and separation of deminamicin

[0033] The fermented liquid obtained in Example 2 was filtered through gauze, the filtrate was extracted with ethyl acetate, concentrated and dried in vacuo to obtain black crude extract F1. Suspend the crude extract F1 in water, extract with petroleum ether, ethyl acetate, and n-butanol in sequence, and concentrate the obtained ethyl acetate extract to obtain extract F2. Perform silica gel column chromatography on extract F2, and use 4 times of volume of chloroform, chloroform:methanol=100:1, chloroform:methanol=100:2, chloroform:methanol=100:4, chloroform:methanol=100:8, chloroform:methanol=100:16, methanol was eluted, concentrated 100:4 (chloroform:methanol) section to obtain black extract F3, and then Sephadex LH-20 separation of extract F3 (eluent is pure methanol), a total of 11 bottles were obtained, and 7-9 bottles were combined to obtain extract F4 , the extract F4 was separated with a reversed-phase sil...

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Abstract

The invention belongs to the technical field of microbial engineering, and particularly relates to deminamicin extracted from fermentation solution of Odontotermes formosanus (Shiraki) endogenous actinomyces and a preparation method and application thereof. The deminamicin has strong inhibiting effect on helicobacter pylori, so the deminamicin can be used as a helicobacter pylori resistant compound and is applied in preparation of novel antibiotic medicaments.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, in particular to Odontotermes formosanus (Shiraki) A new macrolide (deminamicin) extracted from the fermentation broth of endophytic actinomycetes and its preparation method and application. Background technique [0002] Actinomycetes can produce a variety of biologically active substances, which have played a huge role in the establishment and development of the antibiotic industry. After years of research, the number of lead compounds isolated from actinomycetes has dropped sharply, and the probability of discovering new antibiotics has also been greatly reduced. Actinomycetes from special habitats have aroused the interest of some researchers, and the study of secondary metabolites of actinomycetes in special habitats has become an important way to screen new antibiotics. Insects are a kind of biological resource with wide distribution, huge variety and quantity on the earth. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C07D493/10C12P17/18A61K31/365A61P31/04C12R1/465
Inventor 谭仁祥毕淑峰梅亚宁宋勇春郭也李方
Owner NANJING UNIV
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