Bacillus licheniformis with high protease activity and applications thereof
A technology of Bacillus licheniformis and high protease, applied in the field of bioengineering, can solve the problems of unseen geographical populations of Bacillus licheniformis in Hainan Island, poor purification effect, unstable purification effect, etc., and achieve good application prospects and good improvement effect , the effect of high protease activity
Inactive Publication Date: 2011-09-07
HAINAN UNIVERSITY
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Problems solved by technology
However, due to the influence of geographical environment factors (temperature, salinity, pH value), the Bacillus licheniformis strains in different growth environments have a better effect on the purification of aquaculture water in the local environment, but they often show purification effects in the remote environment. Poor effect, unstable purification effect, etc.
[0004] At present, domestic patents related to Bacillus licheniformis microecological preparations are mainly concentrated on fermentation strains for fertilizer production (patent application numbers: 02133713.6, 02114362.5, 02111038.7, 97119176.X, etc.) and feed additives (patent application numbers: 02115969.6, 93110883.7, etc.) In terms of other aspects, there is no patent on Bacillus licheniformis related to the purification of aquaculture water, let alone related patents on the geographical population of Bacillus licheniformis in Hainan Island
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[0021] 1) Preparation of culture medium: use an inoculation loop to pick the target strains that were initially screened, inoculate them in common seawater liquid medium, culture at 30°C at 180rpm for 24h, centrifuge at 6000rpm for 10min, collect 10mL of the supernatant, and filter it with a 0.20μm filter membrane Sterilize, obtain the culture medium of the target strain, and store it at -20°C for later use.
[0022] 2) Determination of protease activity: Prepare a sterilized seawater agar plate containing 5% sterile skim milk, punch holes evenly on the plate with a hole puncher with a diameter of 4 mm, and add 20 μL of the culture solution of the target strain into the holes. An equal amount of 10 μg / ml proteinase K and the culture solution of strains isolated from commercially available beneficial microbial preparations were used as positive controls, and sterile seawater was used as negative controls. Incubate at 30°C for 24 hours, fix with 10% trichloroacetic acid for 2 ho...
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The invention belongs to the technical field of biological engineering and particularly relates to Bacillus licheniformis with high protease activity and applications thereof. The strain was preserved in the China Center for Type Culture Collection (CCTCC) with collection number of CCTCC NO:M2011037 and named as Bacillus licheniformis Pb-WC09005 on January 25, 2011. The strain is separated from the marine environment of the Hainan tropical culture zone, has higher protease activity under the conditions of different temperatures, salinities and pH values, has a better improvement function on the tropical culture aquatic environment and the like, has stable effect and is an strain with good application prospect.
Description
technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to a bacillus licheniformis strain with high protease activity and its application. Background technique [0002] With the continuous expansion of the scale of aquaculture and the continuous improvement of the degree of intensification, outbreaks of aquaculture animal diseases are becoming more and more frequent, and the losses caused by disease outbreaks are also increasing. Although there are many reasons for the occurrence of aquaculture diseases, the outbreak of pathogenic microorganisms and the decline in the immunity of aquaculture animals due to the deterioration of aquaculture water quality are one of the main factors. Improvement has played an important role, but the water quality pollution caused by the discharge of aquaculture wastewater has attracted more and more attention from the society in recent years. How to effectively reduce the discharge of pol...
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Login to View More IPC IPC(8): C12N1/20C02F3/34C12R1/10
Inventor 谢珍玉周永灿王世锋
Owner HAINAN UNIVERSITY