Method for measuring content of free polysaccharides in meningococcal polysaccharide combo
A technology for the content of meningococcus and polysaccharides, applied in biological testing, testing water, measuring devices, etc., can solve problems such as cumbersome operation, large error in measurement results, damage to liver and kidney functions, etc., and achieve the effect of simple and fast operation method
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[0051] Example 1
[0052] 1. Sampling: Take 1 ml of the group A meningococcal polysaccharide conjugate to be tested as sample 1, and take the corresponding derivative solution of the group A meningococcal polysaccharide conjugate as sample 3;
[0053] 2. Centrifugation: Take another 1ml of the meningococcal polysaccharide conjugate and place it in a 1.5ml centrifuge tube. Take 1ml of the derivative solution corresponding to the meningococcal polysaccharide conjugate to be tested and place it in another 1.5ml centrifuge tube. Medium; Add 100μl of 1% DOC solution to each centrifuge tube, keep in ice bath for 30 minutes, then add 25μl of 0.5M HCL solution, shake well, centrifuge at 5000rpm for 15-20 minutes, collect 1ml of supernatant in each centrifuge tube, and combine The substance supernatant was sample 2, and the derivative supernatant was sample 4.
[0054] 3. Calculate the polysaccharide concentration: determine the phosphorus content of sample 1, sample 2, sample 3, and sample ...
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[0061] Example 2
[0062] 1. Sampling: Take 1 ml of the group C meningococcal polysaccharide conjugate to be tested as sample 1, and take the corresponding derivative solution of the group C meningococcal polysaccharide conjugate as sample 3;
[0063] 2. Centrifugation: Take another 1ml of the group C meningococcal polysaccharide conjugate and place it in a 1.5ml centrifuge tube, and take 1ml of the derivative solution corresponding to the group C meningococcal polysaccharide conjugate to be tested and place it in another 1.5ml centrifuge tube Medium; Add 100μl of 1% DOC solution to each centrifuge tube, keep in ice bath for 30 minutes, then add 25μl of 0.5M HCL solution, shake well, centrifuge at 5000rpm for 15-20 minutes, collect 1ml of supernatant in each centrifuge tube, and combine The substance supernatant is sample 2, and the derivative supernatant is sample 4;
[0064] 3. Calculate the polysaccharide concentration: determine the sialic acid content of sample 1, sample 2, sam...
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[0071] Example 3
[0072] 1. Sampling: Take 1 ml of the W135 meningococcal polysaccharide conjugate to be tested as sample 1, and take the corresponding derivative solution of the W135 meningococcal polysaccharide conjugate as sample 3;
[0073] 2. Centrifugation: another 1ml of the W135 meningococcal polysaccharide conjugate is placed in a 1.5ml centrifuge tube, and 1ml of the derivative solution corresponding to the W135 meningococcal polysaccharide conjugate to be tested is placed in another 1.5ml centrifuge tube Medium; Add 100μl of 1% DOC solution to each centrifuge tube, keep in ice bath for 30 minutes, then add 25μl of 0.5M HCL solution, shake well, centrifuge at 5000rpm for 15-20 minutes, collect 1ml of supernatant in each centrifuge tube, and combine The substance supernatant is sample 2, and the derivative supernatant is sample 4;
[0074] 3. Calculate the polysaccharide concentration: determine the sialic acid content of sample 1, sample 2, sample 3, and sample 4, and cal...
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