Method for representing physiological response characteristics of Acorus calamus under diesel stress
A response characteristic, calamus technology, applied in the field of characterization of the physiological response characteristics of calamus to diesel stress, can solve the problems of not forming a standardized and unified characterization method, not many, etc., and achieve the effect of effective risk assessment research
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[0013] Diesel was selected as the single pollutant, and the tolerance range of calamus was obtained through preliminary experiments, and then six parallel concentrations were set, which were 0, 2000, 4000, 6000, 8000 and 10000 mg·kg -1 (Dry sand) mixed into medium (90mm×15mm) filled with 50g of dry sand sample, and 30 sterilized calamus seeds were selected and put into the medium, in artificial climate chamber (28°C, 12 / 12h) Cultivate for 50 days, regularly observe the germination of calamus seeds, and add sterile water in time. During the test, pay attention to keep the water content of each medium consistent, and keep the sandy soil moist and with a certain water content.
[0014] After the calamus grows to 30 days, when the growth of calamus in each culture medium can be clearly distinguished, three calamus plants with the same growth and similar biomass are taken in each petri dish for test 1, and at the same time, they are separately grown in each petri dish. Three calamu...
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