Method for extracting and separating 1-deoxynojirimycin from mulberry leaves

Abstract

The invention discloses a method for extracting and separating 1-deoxynojirimycin from mulberry leaves, which comprises the following steps of: pretreating a raw material, namely crushing a mulberry leaf raw material; performing enzymolysis; extracting by using alkali-alcohol, cooling, filtering to obtain extracting solution; concentrating, and performing acid dissolution and ultrafiltration to obtain ultrafiltrate; removing impurities by using cation resin, namely making the obtained ultrafiltrate pass through the cation resin, eluting by using ammonia alcohol solution, collecting eluent; making the eluent pass through macroporous adsorption resin, eluting by using ethanol solution, and collecting eluent; and concentrating the eluent, adding a methanol-saturated ammonia water mixed solvent, regulating temperature, cooling for crystallization to obtain crystals, and drying. A product with the purity of over 95 percent can be extracted and separated from the mulberry leaf raw material,and the 1-deoxynojirimycin is effectively separated; requirements on equipment are low, and the operation is safe; the process is simple, the used solvents can be recycled, and the production cost isreduced; and the used solvents are low in toxicity, environment-friendly and safe.

Description

technical field [0001] The invention relates to the technical field of separation of effective components of traditional Chinese medicines, in particular to a method for extracting and separating 1-deoxynojirimycin from mulberry leaves. Background technique [0002] Mulberry leaves are the dry leaves of the Moraceae plant mulberry, which are sweet in taste and cold in nature. They are mostly planted in my country and can be used as medicine. With the development of science and technology, people are doing more and more research on mulberry leaves. In recent years, scholars have found that there is a special hypoglycemic substance in mulberry leaves, namely 1-deoxynojirimycin (DNJ) , and the number of diabetic patients in my country and the world is increasing day by day, so 1-deoxynojirimycin in mulberry leaves is more and more favored by researchers. [0003] It is generally believed that the mechanism of 1-deoxynojirimycin for lowering blood sugar is: DNJ is an inhibitor o...

AI Technical Summary

Problems solved by technology

The disclosed method of CN10134586A is to take the initial extract of mulberry leaves as raw material (containing more than 40% of DNJ), after dissolving in water, directly pass through cationic resin, then use eluent to elute and collect step by step, and finally obtain the product with relatively high purity through repeated recrystallization. High product, because the mixed solvent used in recrystallization is not easy to separate and recycle, so the production cost is high

The method disclosed in CN101671294A not only separates and obtains the DNJ product, but also obtains another product, mulberry leaf flavonoids, from mulberry leaves. Specifically, dry mulberry leaves are used as raw materials, extracted with ethanol, centrifugally filtered, and flocculated and precipitated by adding a flocculant; The filtrate is sprayed through the macroporous adsorption resin and cationic resin in turn to obtain the DNJ product. Due to the long process and the use of flocculation and sedimentation, the loss of this method is relatively large, and the final yield is also low. This is the method. biggest shortcoming