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Method for preparing piglet diarrhea-resisting egg yolk antibody microcapsules by spray drying method

A spray-drying method and yolk antibody technology, which is applied in the field of medicine, can solve the problems of research and application of anti-piglet diarrhea yolk antibody microcapsules, specific yolk antibody destruction, and affecting efficacy.

Active Publication Date: 2011-10-05
吉林省正合生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific yolk antibody prepared by the traditional method is easily damaged by gastric acid and pepsin, which affects its efficacy
[0003] At present, there is no literature report on the research and application of anti-piglet diarrhea egg yolk antibody microcapsules prepared by spray drying method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Prepare anti-piglet diarrhea egg yolk antibody microcapsules according to the following process steps:

[0015] (1). Immunize 200 healthy chickens with 1 mL / bird of Escherichia coli inactivated vaccine containing three serotypes of K88, K99 and 987P, and the number of bacteria of each kind of bacteria is more than 3 billion CFU / mL, three times in total , with an interval of 20 days each time. 10 days after each immunization of inactivated E. coli vaccine, followed by immunization with porcine transmissible gastroenteritis, porcine epidemic diarrhea and rotavirus triple attenuated vaccine, at 0.5mL / monkey, for a total of three times, with an interval of 20 days between each time. About 250kg of high-immunity eggs were harvested 10 days after the last immunization;

[0016] (2). Soak the eggs harvested in step (1) with 0.1% bromogeramine by mass percent concentration, disinfect for 30 minutes, then carry out eggshell surface disinfection with 75% alcohol by mass percent ...

Embodiment 2

[0021] The operating steps (1)-(4) of this embodiment are the same as in Example 1. In step (5), the capsule-forming emulsion obtained in step (4) is heated at an inlet temperature of 160° C., a feed rate of 5 ml / min, Spray-dry under the conditions of drying time 7s and outlet temperature 80°C to obtain about 2.5 kg of yolk antibody microcapsules, which are packed in composite paper bags at 0.5 kg / bag to obtain the finished product of anti-piglet diarrhea yolk antibody microcapsules.

Embodiment 3

[0023] The operation steps (1)-(3) of the present embodiment are identical with embodiment 1;

[0024] Operation step (4), after mixing 25000ml of gelatin solution with a concentration of 4% by mass and 25000ml of sodium alginate solution with a concentration of 0.75% by mass, add 500ml of egg yolk antibody obtained in step (3), and then add the final concentration 1% Tween 80, emulsified at a temperature of 65 ° C, a stirring speed of 2000 rpm and a stirring time of 45 minutes to obtain a capsule-forming emulsion;

[0025] Operation step (5) is identical with embodiment 1.

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PUM

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Abstract

The invention discloses a method for preparing piglet diarrhea-resisting egg yolk antibody microcapsules by a spray drying method, which mainly comprises the following steps of: extracting an egg yolk antibody from egg yolk of eggs laid by specific immune healthy hens, and purifying; mixing the egg yolk antibody serving as a core material, gelatin and sodium alginate which serve as a wall material and Tween 80 serving as an emulsifier according to a certain ratio and emulsifying to obtain encystation emulsifying liquid; and finally, performing spray drying on the obtained encystation emulsifying liquid to obtain a microcapsule finished product. In the microcapsule egg yolk antibody prepared by the method, more than 85 percent of activity is kept, the envelop rate is 77.5 percent, the 2-hour release rate of artificial gastric juice is 8.5 percent, the 4-hour release rate of artificial intestinal juice is 81.1 percent, and the egg yolk antibody can be protected against the destruction of gastric acid and pepsase, so that the egg yolk antibody can reach intestinal tracts and can be released to exert effects. The egg yolk antibody microcapsules are a high-efficiency and environment-friendly biological preparation which can replace antibiotics and is used for preventing and treating piglet diarrhea.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for preparing anti-piglet diarrhea egg yolk antibody microcapsules by using a spray drying method. Background of the invention [0002] Piglet diarrhea is one of the main factors causing piglet death and growth retardation, which has seriously endangered the development of my country's pig industry. There are many pathogens that cause diarrhea in piglets, mainly enterotoxigenic Escherichia coli (ETEC), porcine transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), rotavirus and so on. Specific yolk antibody treats and prevents intestinal diseases caused by bacteria and viruses. It has the advantages of safety, no residue, no drug resistance, low cost and convenient administration. It is a kind of highly effective biological agent that can replace antibiotics in the treatment and prevention of piglets The role in diarrhea has attracted the attention o...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61K9/50A61P31/04A61P31/12A61P1/12
Inventor 吴健敏谢彬梁安莉刘文娟覃绍敏陈凤莲黄红梅马玲白安斌袁书智
Owner 吉林省正合生物技术有限公司