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Small bioreactor

A bioreactor and small-scale technology, applied in the field of bioengineering, can solve the problems that manual detection of cell culture density cannot realize the overall repeatability of the overall automatic control system and affect the stability of the system, so as to improve stability and repeatability and reduce friction Loss, the effect of reducing operating energy consumption

Inactive Publication Date: 2011-10-12
北京新节点生物工程技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The electric pump used for liquid replenishment generally uses consumable pipes, and now frequent startup and shutdown will affect the stability of the system
Manual detection of cell culture density cannot achieve overall automatic control and overall system repeatability

Method used

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  • Small bioreactor
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Comparison scheme
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Embodiment 1

[0027] Bioreactors with a working volume of 1.0-1.5 liters.

[0028] according to figure 1 The bioreactor is designed, and the use environment is a room temperature environment of 22-25°C. First, the cell culture tank 21 is made of inorganic glass material: the radius of the spherical segment is 10 cm, the height is 8 cm, and the radius of the connecting cylinder is 10 cm, and the height is 6 cm. Use inorganic glass to process the top cover 22 of the tank body, use sealing rubber pads and fasteners 23 to assemble with the cell culture tank body, and open at the center of the top cover to access the top cover sealing ring 34 .

[0029] After the light source 31 is assembled with the light guide rod 32, the top cover seal ring is used to make the light guide rod close to the bottom of the tank for a certain distance; the air filter device 49 and the external culture solution inflow pipeline 50 are connected through another top cover seal ring.

[0030] The inner airtight cylin...

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a small bioreactor which comprises a culture tank body, a temperature control kit, a liquid (gas) supplying device, a cell optical density detection device and a culture control device. The small bioreactor can meet steam sterilization requirements by adopting the small culture tank body made from inorganic materials as a cell culture main component, and inorganic glass and Teflon materials as main materials of the liquid supplying device, provide optimization conditions for cell culture by matching a high-precision temperature control system with the cell optical density detection device. In the invention, the cell culture main component has high surface smoothness so as to reduce the surface friction loss on cells to a greatest extent; and the small bioreactor has the characteristics of high integration, automatic control, amplification, high reliability, energy conversation, environmental friendliness and suitability for demands on small medium-scale cell culture and gene recombination protein expression in a bioengineering laboratory and pilot plant test production.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to a tank-type bioreactor for cell culture. Background technique [0002] Conventional small tank bioreactors usually use the following methods: [0003] 1) Use a built-in heating device or put the reactor as a whole into an incubator to maintain the culture temperature; [0004] 2) Directly stir the culture by magnetic stirring or gas lifting method, that is, use the gas entering from the opening at the bottom of the tank to form bubbles to rise and mix with the culture to play a stirring role; [0005] 3) Manual sampling is used to detect the cultivation process; [0006] 4) Electric pump is used for rehydration; [0007] 5) The bioreactor is sterilized by in-situ steam sterilization. [0008] Conventional methods have certain limitations in practical use. The steam sterilization method of conventional bioreactors cannot effectively solve the dead angle problem, and the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/02C12M1/36
CPCC12M23/00C12M23/20C12M29/06C12M41/12C12M41/36
Inventor 张俊山黄娜刘士江王堃
Owner 北京新节点生物工程技术有限责任公司
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