Dioscorea alata glycoprotein and preparation method thereof

A technology for ginseng potato sugar and glycoprotein, which is applied in the field of plant functional ingredients and food additives, and can solve problems that have not been reported in the literature

Inactive Publication Date: 2011-10-19
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no literature report on the related data of ginseng potato glycoprotein

Method used

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  • Dioscorea alata glycoprotein and preparation method thereof
  • Dioscorea alata glycoprotein and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] A preparation method of fresh ginseng potato glycoprotein, comprising the steps of:

[0075] (1) Take 100g of fresh ginseng potatoes, wash them, peel them, and cut them into 10cm long pieces;

[0076] (2) Add 500mL of 0.5% NaCl solution to the ginseng cubes obtained in step (1), crush them with a masher, then add 1000mL of 0.5% NaCl aqueous solution, stir them evenly and pour them into the extraction container. °C for 2 h to obtain the extract.

[0077] (3), centrifuge the extract obtained in step (2) at 5000r / min for 15 min, and collect the supernatant;

[0078] (4) Concentrate the supernatant from step (3) to 150mL under reduced pressure. Add 125mL of chloroform and n-butanol mixture with a volume ratio of 4:1, place in a water bath shaker at 35°C, shake at 80r / min for 10min, centrifuge at 5000r / min for 15min, and collect the supernatant;

[0079] Then add 125mL of chloroform and n-butanol mixed solution of chloroform and n-butanol in a volume ratio of 4:1 to the s...

Embodiment 2

[0092] A preparation method of dry ginseng potato glycoprotein, comprising the steps of:

[0093] (1) Weigh 50g of dried ginseng potato slices, crush them, and pass through a 60-mesh sieve;

[0094] (2) Add 250ml of 0.5% NaCl solution to the ginseng powder, stir well, then add 1000ml of 0.5% NaCl solution, stir well, and extract at 45°C for 1.5h to obtain the extract;

[0095] (3) Centrifuge the extract obtained in step (2) at 4000r / min for 20 min, and collect the supernatant;

[0096] (4) Concentrate the supernatant obtained in step (3) to 125ml under reduced pressure, add 100ml of a mixture of chloroform and n-butanol with a volume ratio of 4:1, and place in a water bath shaker for 30~40 Shake at ℃, 80r / min, 10min, centrifuge at 5000r / min for 10min, collect the supernatant, add 100ml of chloroform and n-butanol mixed solution of chloroform and n-butanol in a volume ratio of 4:1 to the supernatant , placed in a water bath shaker at 40°C, 80r / min, shaken for 10 minutes, cent...

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Abstract

The invention discloses a dioscorea alata glycoprotein belonging to the field of plant active substances in the phytochemistry. The glycoprotein is prepared from a dioscorea alata raw material. A preparation method comprises the steps of obtaining a crude glycoprotein by using digestion, concentration, heteroproteins removal and alcohol precipitation and obtaining the dioscorea alata glycoprotein through DEAE-52 (Diethylaminoethyl-52) cellulose column chromatography and SephadexG-75 column chromatography purification, concentration and freeze drying steps. A monosaccharide in the dioscorea alata glycoprotein disclosed by the invention comprises L-galactose, D-glucose and D-mannose; and the molecular weight of the dioscorea alata glycoprotein is about 12500-14500 Da. The glycoprotein can be used for preparing functional foods and health care products and can be also used for studying the bioactivity like a function of lowering blood sugar, a function of adjusting immunity and other physiological functions.

Description

technical field [0001] The invention relates to a ginseng potato glycoprotein and a preparation method thereof, belonging to the field of phytochemistry, in particular to plant functional components and food additives. technical background [0002] Ginseng ( Dioscorea alata L ) belongs to the Dioscoreaceae plant, also known as big potato, red cut potato, etc., mainly distributed in China, Guangxi, Jiangxi, Hunan, Hubei, Yunnan, Guangdong, Guizhou, Sichuan and other regions. At present, bases have been planted in Zhejiang, Jiangsu, Yunnan, Jiangxi, Shandong and other regions, and the resources are relatively rich. Ginseng potato tuber Ganping is non-toxic, and it is a traditional medicinal and edible plant. It has the effects of nourishing the spleen and lungs, astringent essence, reducing swelling and relieving pain. Modern studies have shown that ginseng potatoes are rich in nutrients such as starch, amino acids, vitamins and minerals, and also contain glycoproteins ( G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/16C07K1/14A23L1/305
Inventor 龚钢明将维何婷婷
Owner SHANGHAI INST OF TECH
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