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Method for extracting garlic superoxide dismutase from garlic sheet processing waste water

A technology for processing wastewater and superoxide, applied in biochemical equipment and methods, enzymes, enzymes, etc., to achieve strong market competitiveness, low production costs, and reduce COD

Inactive Publication Date: 2013-01-30
SHANDONG SHUNBANG BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the extraction of garlic superoxide dismutase (SOD) from garlic processing wastewater by using molecular imprinting technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023]With multiple 400-mesh stainless steel screens as the supporting material and 200-mesh diatomite as the filter medium, the diatomite liquid is circulated through the pump, and a 5mm thick diatomite filter layer is evenly coated on the stainless steel screen in advance. Then 10 cubic meters of garlic slice processing wastewater was filtered to remove large particles of impurities and colloidal substances in the garlic slice processing wastewater; The impurity protein in the flake processing wastewater is denatured and flocculated, cooled to 30°C, and then filtered through a diatomaceous earth membrane to remove the impurity protein in the garlic flake processing wastewater; then the garlic flake processing wastewater is filtered and clarified, and then pumped into The resin column is equipped with 1000 kg of superoxide dismutase (SOD) molecularly imprinted resin, and the injection flow rate is controlled to 500 liters / hour, which selectively adsorbs garlic superoxide dismu...

Embodiment 2

[0028] With multiple 400-mesh stainless steel screens as the supporting material and 200-mesh diatomite as the filter medium, the diatomite liquid is circulated through the pump, and a 10 mm thick diatomite filter layer is evenly coated on the stainless steel screen in advance. Then 10 cubic meters of garlic slice processing wastewater was filtered to remove large particles of impurities and colloidal substances in the garlic slice processing wastewater; The impurity protein in the flake processing wastewater is denatured and flocculated, cooled to 30°C, and then filtered through a diatomaceous earth membrane to remove the impurity protein in the garlic flake processing wastewater; then the garlic flake processing wastewater is filtered and clarified, and then pumped into The resin column is equipped with 1000 kg of superoxide dismutase (SOD) molecularly imprinted resin, and the injection flow rate is controlled to 500 liters / hour, which selectively adsorbs garlic superoxide di...

Embodiment 3

[0033] With multiple 400-mesh stainless steel screens as the supporting material, and 200-mesh diatomite as the filter medium, the diatomite liquid is circulated through the pump, and an 8mm thick diatomite filter layer is evenly coated on the stainless steel screen in advance. Then filter 10 cubic meters of garlic slice processing wastewater to remove large particles of impurities and colloidal substances in the garlic slice processing wastewater; pour the filtered garlic slice processing wastewater into a reactor, heat it to 70°C, and heat for 18 minutes to make garlic The impurity protein in the flake processing wastewater is denatured and flocculated, cooled to 30°C, and then filtered through a diatomaceous earth membrane to remove the impurity protein in the garlic flake processing wastewater; then the garlic flake processing wastewater is filtered and clarified, and then pumped into The resin column is equipped with 1000 kg of superoxide dismutase (SOD) molecularly imprin...

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Abstract

The invention discloses a method for extracting garlic superoxide dismutase from garlic sheet processing waste water. The method comprises steps of diatomite membrane filtration, removal of impurity proteins by heating, superoxide dismutase molecular imprinting resin adsorption, eluent condensation and freeze drying, so as to obtain a garlic superoxide dismutase dry powder. According to the method for extracting garlic superoxide dismutase from garlic sheet processing waste water provided by the invention, the extracted garlic superoxide dismutase has high purity, low production costs, so that garlic processing waste water can discharged with standard reached, besides the garlic processing waste water resource is utilized effectively to extract valuable resources in waste water.

Description

technical field [0001] The invention relates to the field of agricultural product processing waste resource recovery, development and utilization, in particular to a method for extracting garlic superoxide dismutase from garlic slice processing wastewater. Background technique [0002] Garlic (Allium sativum L) is a plant of the genus Allium in the Liliaceae family, which can be used for both medicine and food. The chemical components of garlic are mainly divided into thiosulfinates, S-alkane (ene)-L-cysteine ​​derivatives, sexual components, γ-L-glutamic acid polypeptides, glycosides, polysaccharides, lipids , enzymes, saponins. At present, the development and utilization of garlic is one of the hot spots of domestic and foreign medical research. [0003] At present, China is the world's largest garlic producer, consumer and exporter. Garlic has been exported to 131 countries and regions including the United States, Germany and Japan. According to relevant information, i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/08
Inventor 郑永军陈华郑康李延团
Owner SHANDONG SHUNBANG BIOTECH