Early stage prostate cancer semi-quantitative diagnosis method utilizing double indication line immunity chromatography

A technology of immune chromatography and prostate cancer, applied in the field of bioengineering, can solve the problems of missed early treatment and missed diagnosis of patients, and achieve the effect of easy promotion and application, strong pertinence and high accuracy

Inactive Publication Date: 2011-10-19
正元盛邦(天津)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for the differences in normal PSA values ​​at different ages in clinical practice, using the standard of 10 ng/mL alone will cause missed diagnosis and cause many patie...

Method used

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  • Early stage prostate cancer semi-quantitative diagnosis method utilizing double indication line immunity chromatography
  • Early stage prostate cancer semi-quantitative diagnosis method utilizing double indication line immunity chromatography
  • Early stage prostate cancer semi-quantitative diagnosis method utilizing double indication line immunity chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] 1. Prepare PSA monoclonal antibodies 1 and 2. And clean the glassware needed for the experiment.

[0048]2. Preparation of colloidal gold

[0049] First heat the 0.02% chloroauric acid solution to boil, then quickly add 2% trisodium citrate 2mL;

[0050] The color of the solution changed from light blue to dark blue. Continue heating to turn into wine red, continue to boil for 10 minutes; turn to transparent wine red, stop heating and continue to stir to room temperature. In this way, a 20 nm colloidal gold solution was prepared. Then use an electron microscope to check to ensure that the prepared gold particles are as consistent and uniform in size as possible, and the particle diameter is about 20nm, otherwise it will be remade.

[0051] 3. Preparation of colloidal gold markers

[0052] Take two 1.5mL test tubes, add 1mL colloidal gold solution respectively; add appropriate amount of borax buffer solution to adjust the pH to 8.8; add 20μg / mL PSA monoclonal antibod...

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Abstract

The invention discloses a detection method in the technical field of biological engineering, and specifically provides an early stage prostate cancer semi-quantitative diagnosis method utilizing double indication line immunity chromatography. Prostate cancer is a frequently-occurring disease among middle-aged and old males. According to statistics, prostate cancer has the highest incidence of disease in male cancer patients, and is the third cause of death following lung cancer and colorectal cancer. Currently, a PSA detection method used clinically is an ELISA and a CLIA method, etc. the above methods are time-consuming, energy-consuming and is not conducive to popularization and is expensive. An immunization colloidal gold technology has developed rapidly and has been applied widely to detections of infectious disease, early pregnancy and cancer, etc. The invention employs a colloidal gold immunity chromatography to establish a PSA rapid detection method which can realize a semi-quantitative determination of 4ng/mL-10ng/mL PSA ambiguous region and guide a prostate biopsy according to age.

Description

technical field [0001] The invention relates to a detection method in the technical field of bioengineering, in particular to a method for semi-quantitative diagnosis of early prostate cancer by double indicator line immunochromatography. Background technique [0002] Prostate cancer (PCa) is a common and frequently-occurring disease in middle-aged and elderly men, accounting for 10% to 20% of all types of cancer in men, and is the most common cancer in men. According to statistics, among male cancer patients, prostate cancer has the highest incidence rate and is the third cause of death besides lung cancer and colorectal cancer. The disease progresses slowly and is the main cancer that threatens the lives of men over 50 years old. It accounts for the second highest male cancer death rate in western countries, and the first male cancer incidence rate in northern European countries. The incidence rate in the United States is 14.3%. , second only to lung cancer, accounting fo...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 霍五奎岳晓燕马雪明刘寅王晶罗云萍张有青
Owner 正元盛邦(天津)生物科技有限公司
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