Cochlear greater epithelial ridge (GER) cell line and its application

Abstract

The invention discloses a cochlear GER cell line, the preservation number of which is CGMCC No.4719. The cochlear GER cell line is obtained through the steps of transfection of SV40 large tumor antigen into a cochlear GER cell through a viral vector, introduction of the eukaryotic expression plasmid prv5-EGFP of the encoding gene Math1 (mammalian atonal homolog1), screening with G418 to obtain ananti-G418 positive cell, amplification culture of the anti-G418 positive cell and screening. The cell line has a fast growing speed, is easy to culture, can express characteristic marking protein myosin VIIa of hair cells when introduced to a cochlea model for deafening caused by noise, and can grow to be a stereocilia bundle with the characteristics of hair cells. The cell line is convenient forpopularization and is applicable to research on differentiation and regeneration of hair cells.

Description

technical field

[0001] The invention relates to the field of cell line construction, in particular to a cochlear large epithelial crest cell line and application thereof. Background technique

[0002] Deafness is the sensory or functional defect disease with the highest incidence rate in humans. It not only seriously affects the quality of life, study, social interaction and family life of the affected population, but also brings physical and psychological pain to patients and their families. Deafness is divided into conductive deafness, sensorineural deafness and mixed deafness. According to the survey results released by the Ministry of Health of my country in 2000, sensorineural deafness accounts for about 63% of deaf patients, and its absolute number exceeds 80 million. At present, the treatment of sensorineural hearing loss mostly requires the help of cochlear implants. The clinical application of cochlear implantation and hearing aid technology has partially improved...

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