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Method for pollination and fructification of orchids in test tubes

An in-vitro, plant technology, applied in the biological field, can solve problems such as long breeding cycle, achieve the effects of low cost, improve breeding efficiency, and shorten breeding cycle

Inactive Publication Date: 2011-11-16
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of long conventional breeding cycle of orchids, and provide a set of methods for regulating plant flowering, artificial pollination and fertile seeds in in vitro culture. This technology can effectively shorten the breeding cycle and improve breeding efficiency.

Method used

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Embodiment

[0016] Embodiment: In this embodiment, the Orchidaceae plant Dendrobium candidum is taken as an example to illustrate the method for in vitro pollination and fruiting of the Orchidaceae plant, but the present invention is not limited to the embodiments.

[0017] The material is the mature uncracked capsule of Dendrobium officinale. Remove the fruit stalk from the harvested fruit, rinse it with tap water, and then use a volume ratio of 75% ethanol to disinfect the surface for 30 seconds on a sterile ultra-clean workbench, rinse it twice with sterile water, and use 0.1% HgCl by weight. 2 Soak in water for 15-20 minutes, rinse with sterile water 3-4 times. Place the washed capsules on sterilized filter paper to absorb the moisture. Use a sterile scalpel on an ultra-clean workbench to cut off the front and rear ends of the capsule at 1 cm each, cut the middle section longitudinally, and evenly sprinkle the dried powdered seeds in the capsule on the MS medium. The culture tempera...

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Abstract

The invention relates to a method for pollination and fructification of orchids in test tubes. In the invention, second filial generation plants are obtained through a process of orchid tissue culture, so the breeding period of the orchids especially Dendrobium candidum is effectively shortened. The breeding period of 4-5 years in a natural state can be shortened to less than one year, and the method of the present invention which has the advantages of short culture time and low cost is in favor of shortening the breeding period of orchid and improving the breeding efficiency.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tissue culture method, in particular to a method for in vitro pollination and fruiting of orchid plants. Background technique [0002] Orchids (orchids) have huge economic value and are an important part of the flower industry in the world, and many species also have high medicinal value. Wherein the commonly used asexual reproduction method is branch propagation, and the breeding cycle of seed propagation is long, generally needs 4-5 years. And, generally, the work of cultivating new varieties of orchids requires repeated hybridization, backcrossing and selfing in order to achieve the purpose of breeding and select and breed excellent new varieties. Using conventional methods, this breeding process generally takes more than ten years. And flowering, pollination and bearing fruit in test tube of orchid can solve this problem well. With the method of promoting flowering and artif...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 田敏王彩霞欧阳彤
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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