Method and pair of special primers for identifying purple properties of Chinese cabbages

A primer pair and Chinese cabbage technology, which is applied in biochemical equipment and methods, microbiological measurement/testing, DNA/RNA fragments, etc., can solve the problems of not meeting the requirements of molecular marker-assisted breeding, short primers, and high cost, and achieve The identification results are accurate and reliable, the effect of speeding up the breeding process and reducing labor costs

Inactive Publication Date: 2011-11-16
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

Although Zhang Mingke found a marker linked to the purple trait, its genetic distance is still relatively far away, far from meeting the requirements of molecular marker-assisted breeding
[0008] At present, among the applied labels, the amount of DNA required for RFLP labeling is large, the steps are many, and the cycle is long. Radioactive isotopes are used in the preparation of probes and detection, and the cost is high; RAPD labeling uses relatively short primers. The reaction conditions are extremely sensitive, a slight change wi

Method used

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  • Method and pair of special primers for identifying purple properties of Chinese cabbages
  • Method and pair of special primers for identifying purple properties of Chinese cabbages
  • Method and pair of special primers for identifying purple properties of Chinese cabbages

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Embodiment 1

[0028] Embodiment 1, the application of molecular marker

[0029] 1. Materials

[0030] Chinese cabbage (09-680) in the literature "Zhang Deshuang, Zhang Fenglan, Zhao Xiuyun, Yu Yangjun, Xu Jiabing, Yu Shuancang, Wang Weihong. 2011. Genetic analysis of purple traits in leaves of Chinese cabbage (Chinese cabbage × purple cabbage). Chinese Vegetables, 2: 36-38", the public can obtain from Beijing Academy of Agriculture and Forestry Sciences.

[0031] Purple pakchoi (09N-742) in the literature "Zhang Deshuang, Zhang Fenglan, Zhao Xiuyun, Yu Yangjun, Xu Jiabing, Yu Shuancang, Wang Weihong. 2011. Genetic analysis of purple traits in leaves of Chinese cabbage (Chinese cabbage × purple pakchoi). Chinese Vegetables, 2 : 36-38", the public can obtain from Beijing Academy of Agriculture and Forestry Sciences.

[0032] Chinese cabbage P 1 (09-680) and P 2 F 1 , and then self-cross to get F 2 Generation, with 186 F 2 Single plant construction F 2 group( figure 1 ).

[0033] 2. ...

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Abstract

The invention discloses a method and a pair of special primers for identifying purple properties of Chinese cabbages. One of the primers is shown in SEQ ID NO:1, and the other one of the primers is shown in SEQ ID NO:2. In the invention, by using the F2 colony for controlling the genes of the purple properties to be positioned on the A3 chromosome for the first time, molecular markers which are closely linked with the purple properties are found, and the molecular markers can be used for identifying the purple properties of Chinese cabbages, thereby laying the foundations for performing molecular marker-assisted breeding and cloning genes. The identification method disclosed by the invention has accurate and reliable identification results and can be used for identifying in the early stage of growth of Chinese cabbages without waiting for the maturation of plants, thereby saving the time, lowering the labor cost, increasing the breeding speed and having important significance.

Description

technical field [0001] The invention relates to a method for identifying purple traits of cabbage and a pair of special primers. Background technique [0002] Anthocyanins are water-soluble pigments that exist in plants. They consist of a basic structural core and different substituents. Due to the different types and positions of substituents, anthocyanins of different colors are formed, including pink, red, and purple. and blue, about 88% of the flower color of angiosperms is determined by anthocyanins, which are mainly accumulated in plant vacuoles and have a variety of physiological functions, such as attracting insects for pollination, resisting low temperature and ultraviolet damage, and Prevention and treatment of plant diseases, etc.; anthocyanin is the most powerful free radical scavenger found so far, with anti-oxidative aging, anti-mutation, anti-cancer and anti-arteriosclerosis functions; in addition, anthocyanin is a safe, non-toxic Toxic natural food coloring....

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 汪维红张德双张凤兰于拴仓赵岫云余阳俊刘瑾徐家炳卢桂香
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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