Primer library and screening system for rapid PCR (Polymerase Chain Reaction) detection for population sudden viral epidemics

A virus and virology technology, applied in special data processing applications, instruments, electronic digital data processing, etc., can solve problems such as inability to design primers, amplify virus sequences, and inability to well meet virus primer design.

Inactive Publication Date: 2011-11-16
解放军第三〇二医院 +1
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AI Technical Summary

Problems solved by technology

In addition, for a certain disease, there may be multiple viruses acting together to cause the disease, so it is impossible to design suitable primers to amplify the virus sequence before knowing the specific virus species, so the conventional primer design method cannot be very good. Fully meet the needs of viral primer design

Method used

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  • Primer library and screening system for rapid PCR (Polymerase Chain Reaction) detection for population sudden viral epidemics
  • Primer library and screening system for rapid PCR (Polymerase Chain Reaction) detection for population sudden viral epidemics
  • Primer library and screening system for rapid PCR (Polymerase Chain Reaction) detection for population sudden viral epidemics

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Embodiment Construction

[0010] 1. Clinical virology database module

[0011] The clinical virus database contains clinical information, treatment information and basic virus information of common viral diseases. Using this database, all possible virus sources can be obtained through clinical information, providing a data source for the next step of obtaining viral genome information. In addition, for diseases caused by new virus species or new subtypes of known viruses, the most comprehensive clinical information data will be integrated into this database at the same time as the disease is released.

[0012] 2. Virus sequence database module

[0013] Download the customer-specified viral genome sequence that has been sequenced and published, and construct a sequence database, which is mainly from GenBank and EMBL. The sequence database storage format is Fasta format. Through the integration of 3309 reference sequences of 2235 viral genomes and 39 reference sequences derived from viroids that have ...

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Abstract

The invention relates to a primer library and screening system for rapid PCR (Polymerase Chain Reaction) detection for population sudden viral epidemics. The invention relates to a viral primer design and assessment system and belongs to the field of combination of technologies of biology and computer databases. The primer library and screening system mainly comprises a clinical virology database module, a virus sequence database module, a virus sequence automatic extraction module, a virus variation sequence function prediction module, a virus species specificity sequence and virus subtype specificity sequence screening module, a primer generation and evaluation module and an amplification product interpretation module. The invention aims to achieve the following purposes: firstly, an optimal PCR-amplified virus primer sequence is designed according to a target gene sequence; secondly, the specificity and accuracy of the primer are ensured; thirdly, the matching degree between an amplified fragment and a target virus is predicted under the condition that sequencing is not carried out; and fourthly, the clinical background data and referenced therapeutic schedules of a virus are predicted according to the amplified sequence of the PCR primer.

Description

technical field [0001] The invention is a virus primer design and evaluation system, which belongs to the field of combination of biology and computer database technology. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction, PCR) is a method for rapidly amplifying DNA in vitro, used to amplify specific DNA fragments, within a few hours, the target gene fragment can be amplified to millions of copies of molecular biology learn technology. The basic principle of PCR technology is similar to the natural replication process of DNA, and its specificity depends on oligonucleotide primers complementary to both ends of the target sequence. PCR consists of three basic reaction steps: denaturation-annealing-extension: ① Denaturation of template DNA: After the template DNA is heated to about 93°C for a certain period of time, the double-stranded DNA of the template or the double-stranded DNA formed by PCR amplification is decomposed. ②Annealing (anneali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/28C12Q1/68
Inventor 程云李林李伯安戚杨迟淑萍韩晋苏峰张永强
Owner 解放军第三〇二医院
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