Pseudomonas and microbial agent as well as applications thereof

A microbial inoculum, a technology of Pseudomonas, which is applied in the field of Pseudomonas and can solve problems such as low degradation ability

Inactive Publication Date: 2011-11-23
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of existing microbial strains with low degradation ability to petroleum hyd

Method used

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  • Pseudomonas and microbial agent as well as applications thereof
  • Pseudomonas and microbial agent as well as applications thereof
  • Pseudomonas and microbial agent as well as applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Enrichment and isolation of petroleum hydrocarbon degrading bacterial strains

[0040] Weigh 5g of the oil-contaminated soil mixture and add it to a 250mL Erlenmeyer flask filled with 100mL enrichment medium, cultivate it at 28°C and 180r / min on a constant temperature shaker, and transfer 5mL of the culture medium to a fresh enrichment culture after 5 days base, cultured under the same conditions for 5 days, and continuously enriched and cultured for 25 days in this way, the culture solution was diluted serially and spread on solid PDA medium, and cultured at 28°C; after colonies grew on the plate, single colonies of different shapes were picked Separation and purification were carried out repeatedly by streak separation until a pure culture of a single strain was obtained, and the purified strain was preserved in the slant of beef extract peptone medium.

[0041] 2. Screening of strains with high degradation activity

[0042] Inoculate the above-mentioned purified ...

Embodiment 2

[0046] According to the experimental content and experimental methods recorded in the "Common Bacteria System Identification Manual" [4] and "Principles, Methods and Practices of Actinomycete Systemology" [5], the strains with the preservation number CGMCC No. 4793 were screened. Bacteria identification.

[0047] 1. Morphological analysis

[0048] The preservation number is CGMCC No. 4793 bacterial strain carries out morphological analysis and observes under optical microscope, and the results are as follows: figure 1 and 2 shown.

[0049] The observation of the colony morphology was carried out on solid TSA medium. After the strains were streaked and inoculated, they were cultured at a constant temperature of 28°C for 24 hours to observe the colony morphology. The colony of the strain with the preservation number CGMCC No. 4793 is colorless and translucent, round, with irregular edges, smooth texture and flat surface. After picking and culturing for 24 hours, the bacteri...

Embodiment 3

[0062] Weigh 10g of petroleum-contaminated soil mixture (1.25g of petroleum content) and add it to 100mL of beef extract peptone medium (preservation number is CGMCC No. 4793). 0.5×10 10 1) in a 250mL Erlenmeyer flask, cultured at 28°C and 180r / min on a constant temperature shaker. After 3 days, the oil content in the oil-contaminated soil mixture was detected, and the result was 0.51 grams. The degradation rate of the strain to oil was about 59.2 %.

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PUM

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Abstract

The invention provides pseudomonas with the collection number of CGMCC (China General Microbiological Culture Collection Center) No.4793, a microbial agent containing the pseudomonas, and applications of the pseudomonas or the microbial agent in petroleum hydrocarbon degradation, petroleum pollution bioremediation, petroleum polluted soil bioremediation and petroleum polluted water bioremediation. The pseudomonas with the collection number of CGMCC No.4793 and the microbial agent containing the pseudomonas have strong degrading capability for the petroleum hydrocarbon, can be widely applied to petroleum hydrocarbon degradation and petroleum pollution renovation, petroleum polluted soil renovation, petroleum polluted water renovation and the like.

Description

technical field [0001] The present invention relates to a kind of Pseudomonas ( Pseudomonas ), and a species containing the Pseudomonas ( Pseudomonas ) microbial agents as active ingredients and their applications; more precisely, it involves a Pseudomonas ( Pseudomonas ), and the microbial agent containing this Pseudomonas, also relates to the application of said Pseudomonas or microbial agent in the degradation of petroleum hydrocarbons, the application in bioremediation of oil pollution, the application of bioremediation of oil pollution The application in the soil, and the application in bioremediation of oil-contaminated water bodies. Background technique [0002] In today's world, petroleum has become one of the most important energy sources for human beings. A large amount of petroleum and its processed products enter the soil, causing petroleum pollution in the soil, which brings harm to human beings and even the entire biosphere, thus becoming a worldwide environm...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C02F3/34B09C1/10C12R1/38A62D101/20C02F103/34
Inventor 郭萍田云龙朱昌雄刘雪李峰叶婧
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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