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A kind of method for preparing bacterial cellulose with Jerusalem artichoke as carbon source

A technology of bacterial cellulose and Jerusalem artichoke, which is applied in the field of bacterial cellulose preparation, can solve the problems of high cost, low price, and low output, and achieve the effects of low cost, reduced production raw material cost, and accelerated synthesis

Active Publication Date: 2017-04-05
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main obstacle to the large-scale utilization of bacterial cellulose is its low yield, high cost, and price inferior to ordinary cellulose. Therefore, the focus of research is on finding new carbon sources and finding cheap and suitable raw materials, which can not only reduce production costs but also increase production costs. Cellulose Yield

Method used

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  • A kind of method for preparing bacterial cellulose with Jerusalem artichoke as carbon source
  • A kind of method for preparing bacterial cellulose with Jerusalem artichoke as carbon source
  • A kind of method for preparing bacterial cellulose with Jerusalem artichoke as carbon source

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Wash the Jerusalem artichoke with water first, weigh and pack separately, cook at 120°C for 20 minutes, and homogenize it with a juicer. The ratio of Jerusalem artichoke to water (g / ml) is 1:1. Put the Jerusalem artichoke pulp in a water bath at 80°C Leach for 1h. Filter through two layers of gauze to remove the filter residue to obtain clear Jerusalem artichoke juice, centrifuge at 6000r / min for 20min, and refrigerate at 4°C for later use. See the experimental results figure 1 , the total sugar was measured by the phenol-sulfuric acid method, and the yield was recorded as the total sugar concentration multiplied by the volume of the extract solution and divided by the mass of fresh Jerusalem artichoke.

[0049] Add sulfuric acid to the filter residue to a concentration of 0.5wt%, hydrolyze at 110°C for 30min, and use Ca(OH) 2 Adjust the pH value of the hydrolyzate to 9.5, add activated carbon for adsorption, filter out the activated carbon after the reaction and rea...

Embodiment 2

[0053] Wash the Jerusalem artichoke with water first, weigh and pack separately, cook at 80°C for 30 minutes, homogenize it with a juicer, the ratio of Jerusalem artichoke to water (g / mi) is 1:1, put the Jerusalem artichoke pulp in a water bath at 80°C Leach for 2.5h. Filter through two layers of gauze to remove the filter residue to obtain clear Jerusalem artichoke juice, centrifuge at 8000r / min for 20min, and refrigerate at 4°C for later use. See the experimental results figure 2 .

[0054] Add hydrochloric acid to the filter residue to a concentration of 3wt% or add fructanase, pectinase, amylase and cellulase to 500U / mL, hydrolyze at 20°C for 60min, and collect the hydrolyzate for later use.

[0055] Add hydrochloric acid to Jerusalem artichoke juice to a concentration of 3wt% or amylase to 50U / mL, hydrolyze at 20°C for 60min, and collect the hydrolyzate for later use.

[0056] The clarified Jerusalem artichoke juice or hydrolyzate is used as the carbon source of the c...

Embodiment 3

[0058] Clean the fresh Jerusalem artichoke, add water directly to homogenate, the solid-liquid ratio (g / ml) of Jerusalem artichoke to water is 1:8, after homogenization, leaching in water bath at 100°C for 2.0h. Filter through two layers of gauze to remove the filter residue to obtain clear Jerusalem artichoke juice, centrifuge at 8000r / min for 20min, and refrigerate at 4°C for later use. The experimental results are shown in .3. The total sugar was measured by the phenol-sulfuric acid method, and the yield was recorded as the total sugar concentration multiplied by the volume of the extract solution and divided by the mass of fresh Jerusalem artichoke.

[0059] Add sulfuric acid to the filter residue to a concentration of 1wt%, hydrolyze at 100°C for 40min, and use Ca(OH) 2 Adjust the pH value of the hydrolyzate to 11, filter and re-adjust the pH value to 5.5, then add activated carbon for adsorption, filter out the activated carbon after the reaction and fine-tune the pH val...

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Abstract

The invention relates to a method for preparing bacterial cellulose by taking Jerusalem artichoke as a carbon source. The method comprises the following steps: (1) directly adding water in cleaned Jerusalem artichoke to perform homogenate or performing homogenate after steaming cleaned Jerusalem artichoke for 15-60 min at 80-120 DEG.C, leaching after homogenate; filtering to obtain filter residues and filtrate after leaching, centrifuging the filtrate to obtain Jerusalem artichoke juice; (2) respectively hydrolyzing the filter residues and the filtrate to obtain hydrolyzate; (3) taking the hydrolyzate as a culture medium carbon source, adding a nitrogen source to prepare a fermentation medium, inoculating seed liquor of a bacterial cellulose production strain in the fermentation medium to obtain the bacterial cellulose after 2-23 days. The production process provided by the invention has the advantages of wide raw material source, low cost, and strong operability; and the yield of the bacterial cellulose produced by the treated Jerusalem artichoke juice is higher than the yield of the bacterial cellulose produced by other carbon sources; and the method provided by the invention has good application prospect in the production field of bacterial cellulose.

Description

technical field [0001] The invention belongs to the field of bacterial cellulose preparation, in particular to a method for preparing bacterial cellulose by using Jerusalem artichoke as a carbon source. Background technique [0002] As a natural polymer material, bacterial cellulose (BC) has good biocompatibility, biodegradability, strong water holding capacity and high mechanical properties. [0003] In view of the excellent characteristics of bacterial cellulose, bacterial cellulose has extensive and special uses. In the field of medical materials, bacterial cellulose can be used to synthesize artificial skin, artificial blood vessels, surgical dressings, carriers of slow-release drugs, etc.; in the field of food industry, bacterial cellulose itself can be used as a food, such as commonly known as coconut or In addition, BC can also be used as a thickener, forming agent, additive, etc. in the food industry; in the paper industry, the addition of bacterial cellulose can im...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/02C12R1/01C12R1/41C12R1/38C12R1/025C12R1/05C12R1/065
Inventor 洪枫韩筱
Owner DONGHUA UNIV
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