Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Simple production method of rare ginsenoside IH-901

A technology of IH-901 and ginsenoside, which is applied in the direction of fermentation, etc., can solve the problems of no mature method or process suitable for large-scale production, and achieve the effect of low raw material cost, abundant sources, and simple separation and purification of products

Inactive Publication Date: 2011-11-23
HUAQIAO UNIVERSITY
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no mature method or process suitable for large-scale preparation in microbial fermentation preparation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The simple production method of rare ginsenoside IH-901 crystallization of the present invention is realized through the following steps:

[0012] ①Reaction: Precisely weigh ginsenoside Rb 1 1000mg, add pH4.0 phosphate citrate buffer 1000mL, add 150mg helicase, and bathe in 42℃ water for 15h.

[0013] ②Product extraction and concentration: terminate the reaction, add 500mL ethanol for extraction and extraction for 24 hours, centrifuge, and collect the ethanol extract; concentrate the collected ethanol extract on a rotary evaporator under reduced pressure to obtain an extract; dissolve the extract in chloroform , adding water for extraction, taking the chloroform layer, and concentrating the chloroform layer to obtain 800 mg of the column sample.

[0014] ③Column passage: the medium is silica gel G (200 mesh-300 mesh), with CHCl 3 -AcOEt-MeOH-H 2 O (2:4:1:1, lower layer) is the eluent, elute 800 mg of the upper column sample obtained in step ② to 800 ml, and then star...

Embodiment 2

[0018] The simple production method of rare ginsenoside IH-901 crystallization of the present invention is realized through the following steps:

[0019] ①Reaction: Precisely weigh ginsenoside Rb 1 1000mg, add pH4.0 phosphate citrate buffer 1000mL, add helicase 300mg, 35℃ water bath for 10h.

[0020] ②Product extraction and concentration: terminate the reaction, add 500mL ethanol for extraction and extraction for 24 hours, centrifuge, and collect the ethanol extract; concentrate the collected ethanol extract on a rotary evaporator under reduced pressure to obtain an extract; dissolve the extract in chloroform , adding water for extraction, taking the chloroform layer, and concentrating the chloroform layer to obtain 800 mg of the column sample.

[0021] ③Column passage: the medium is silica gel G (200 mesh-300 mesh), with CHCl 3 -AcOEt-MeOH-H 2 O (2:4:1:1, lower layer) is the eluent, elute 800 mg of the upper column sample obtained in step ② to 800 ml, and then start to col...

Embodiment 3

[0025] The simple production method of rare ginsenoside IH-901 crystallization of the present invention is realized through the following steps:

[0026] ①Reaction: Precisely weigh ginsenoside Rb 1 1000mg, add pH4.0 phosphate citrate buffer 1000mL, add helicase 100mg, 50℃ water bath for 20h.

[0027] ②Product extraction and concentration: terminate the reaction, add 500mL ethanol for extraction and extraction for 24 hours, centrifuge, and collect the ethanol extract; concentrate the collected ethanol extract on a rotary evaporator under reduced pressure to obtain an extract; dissolve the extract in chloroform , adding water for extraction, taking the chloroform layer, and concentrating the chloroform layer to obtain 800 mg of the column sample.

[0028] ③Column passage: the medium is silica gel G (200 mesh-300 mesh), with CHCl 3 -AcOEt-MeOH-H 2 O (2:4:1:1, lower layer) is the eluent, elute 800 mg of the upper column sample obtained in step ② to 800 ml, and then start to col...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a simple production method of rare ginsenoside IH-901. The method comprises the following steps: evenly mixing 1 unit mass of a ginsenoside Rb1 monomer, 0.1-0.3 unit mass of domestic crude snail enzyme and 1 unit volume of phosphoric acid-citric acid buffer solution with the pH value of 3-5, performing heat preservation at the temperature of 35-50 DEG C for 10-20 hours, and then terminating the reaction; adding 0.5 unit mass of ethanol into the mixed solution for extraction, centrifugally collecting an ethanol layer, and then decompressing and concentrating to obtain a sample; placing the sample on the silicagel column, taking a lower CHCl3-AcOEt-MeOH-H2O layer as an eluent for eluting 0.8 unit mass of the sample, collecting the obtained elution solution, and then decompressing and concentrating to obtain a condensed extract; and dissolving the condensed extract in a mixed solution of ethanol and water at a volume ratio of 100:70, and staying overnight at room temperature so as to finally obtain IH-901 crystal with the purity more than 95%. In the method, as the ginsenoside Rb1 monomer is taken as a reaction substrate and the cheap domestic crude snail enzyme is taken as the reaction enzyme, the separation and purification process of the product is very simple; and in addition, by utilizing the single substrate and the cheap domestic crude snail enzyme, the simple production method has the advantages of rich enzyme source, low raw material cost, simple product extraction process and the like.

Description

technical field [0001] The invention relates to a simple production method of crystallization of rare ginsenoside IH-901. Background technique [0002] 20-O-β-D-glucopyranoside-20(S)-protopanaxadiol (rare ginsenoside IH-901, also known as M1 or Compound K), which does not exist in natural ginseng and is naturally Degradation metabolites of the diol group ginsenosides in the human gut. In recent years, many studies have shown that many biological activities of ginseng are caused by this compound, especially in anti-tumor, anti-aging, anti-inflammation, etc., which have good medicinal effects. [0003] IH-901 will be a promising anti-tumor drug, and it will be listed in the form of health care products and national new drugs in the future. The rare ginsenoside project has forward-looking, scarce and huge demand in biomedicine and biohealth products in the 21st century , the international and domestic markets have great potential for its demand. At present, rare ginsenoside ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00
Inventor 林毅
Owner HUAQIAO UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com