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High-flux dry biochemical chip

A high-throughput, chip-based technology, applied in the field of dry biochemical detection, biochemical chips, and dry-type biochemical chips of clinical laboratory analysis instruments and diagnostic reagents, can solve the problem of high requirements for operators, poor stability of opening bottles, reagents, etc. High consumption and other problems, to achieve the effect of improving detection efficiency, reducing the amount of detection samples, and reducing the amount of use

Inactive Publication Date: 2011-11-23
钱辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] As a type of dry biochemistry, the biochemical chip is mainly used by medical institutions to conduct qualitative or quantitative analysis of specimens in combination with supporting instruments. Currently, the biochemical detection methods used in the market mainly use liquid-phase turbidimetry, and the supporting instruments are complicated. The requirements are relatively high, the storage period of the reagent is relatively short, the stability of opening the bottle is not good, and the required sample volume is relatively large; each specimen needs to be tested with a separate reagent, the consumption of the reagent is large, the upper limit of the speed has a bottleneck, and the reaction process is easy. cross-contamination

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The first layer: the diffusion layer, which promotes the uniform diffusion of the sample.

[0026] The second layer: filter layer, remove interference, made of nitrocellulose membrane material.

[0027] The third layer: the reagent layer, which makes the sample react with the reagent, which is made of freeze-dried powder reagent, and the reagent is a mixed reagent of Mg ion and AMP.

[0028] The fourth layer: the chromogenic layer, which makes the final reactants show different colors, and is made of freeze-dried powder chromogenic agent, which is p-nitrophenyl phosphate.

[0029] During the reaction, the sample to be detected penetrates through the sample injection hole 21 on the first layer of reaction hole cover plate 2, and enters the reaction hole 11 in the chip base 1 after being filtered by the second layer of filter membrane to react with the reagent of the third layer. Then continue to infiltrate downward and react with the color reagent on the fourth layer un...

Embodiment 2

[0032] The first layer: the diffusion layer, which promotes the uniform diffusion of the sample.

[0033] The second layer: filter layer, remove interference, made of nitrocellulose membrane material.

[0034] The third layer: the reagent layer, which makes the sample react with the reagent. It is made of freeze-dried powder reagent, and the reagent is glycylglycyl peptide.

[0035] The fourth layer: the color development layer, which makes the final reactants show different colors, and is made of freeze-dried powder color developer, and the color developer is γ-glutamyl-p-nitroaniline.

[0036] During the reaction, the sample to be detected penetrates through the sample injection hole 21 on the first layer of reaction hole cover plate 2, and enters the reaction hole 11 in the chip base 1 after being filtered by the second layer of filter membrane to react with the reagent of the third layer. Then continue to infiltrate downward and react with the color reagent on the fourth ...

Embodiment 3

[0039] The first layer: the diffusion layer, which promotes the uniform diffusion of the sample.

[0040] The second layer: filter layer, remove interference, made of nitrocellulose membrane material.

[0041] The third layer: the reagent layer, which makes the sample react with the reagent. It is made of freeze-dried powder reagent, and the reagent is LiOH dry reagent.

[0042] The fourth layer: the chromogenic layer, which makes the final reactants show different colors, and is made of freeze-dried powder chromogenic agent, and the chromogenic agent is copper tartrate.

[0043] During the reaction, the sample to be detected penetrates through the sample injection hole 21 on the first layer of reaction hole cover plate 2, and enters the reaction hole 11 in the chip base 1 after being filtered by the second layer of filter membrane to react with the reagent of the third layer. Then continue to infiltrate downward and react with the color reagent on the fourth layer until the ...

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Abstract

The invention relates to a high-flux dry biochemical chip which comprises a chip base, a filter layer and a reaction hole cover board, wherein a plurality of mutually independent reaction holes are formed on the chip base; a reagent layer and a color development layer are arranged in each reaction hole from top to bottom; sample injection holes are formed on the reaction hole cover board; the reaction hole cover board is arranged on the chip base and the sample injection holes are positioned on the reaction holes; and the filter layer is positioned between the sample injection holes and the reaction holes. Preferably, the chip base is provided with a concave part; the reaction holes are formed in the concave part; the reaction hole cover board is arranged in the concave part; the chip base is a plastic base, a cylindrical base or a transparent base; the number of reaction holes is more than 10; the sample injection holes are distributed on an inner circle and an outer circle; and the filter layer is a filter membrane. The high-flux dry biochemical chip is unique in design and ingenious in idea, so that the detection efficiency can be improved, the using amount of sample is reduced and the cost is reduced; and the high-flux dry biochemical chip is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical field of biochemical chips, in particular to the technical field of dry biochemical chips, specifically a high-throughput dry biochemical chip, which is used in the field of dry biochemical detection of clinical laboratory analysis instruments and diagnostic reagents. Background technique [0002] As a type of dry biochemistry, the biochemical chip is mainly used by medical institutions to conduct qualitative or quantitative analysis of specimens in combination with supporting instruments. Currently, the biochemical detection methods used in the market mainly use liquid-phase turbidimetry, and the supporting instruments are complicated. The requirements are relatively high, the storage period of the reagent is relatively short, the stability of opening the bottle is not good, and the required sample volume is relatively large; each specimen needs to be tested with a separate reagent, the consumption of the reagent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00
Inventor 钱辉
Owner 钱辉
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