Queen bee intestinal enzyme and its preparation method and application

An intestinal and queen bee technology, applied in the field of queen bee intestinal enzymes and preparation thereof, can solve the problems of difficult screening of biologically active polypeptides, unsuitable for royal jelly proteolysis, lack of polypeptides, etc.

Inactive Publication Date: 2011-11-30
BEIJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, so far, there have been few studies on the enzymatic hydrolysis of royal jelly polypeptides, and most of the enzymatic hydrolysis methods are carried out by exogenous enzymes such as pepsin, trypsin, papain, etc., which are not suitable for the hydrolysis of royal jelly protein, and it is difficult to screen to obtain the right peptides. Bioactive peptides that are truly effective for the human body

Method used

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  • Queen bee intestinal enzyme and its preparation method and application
  • Queen bee intestinal enzyme and its preparation method and application
  • Queen bee intestinal enzyme and its preparation method and application

Examples

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Effect test

Embodiment 1

[0065] Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. Add 50mmol / L phosphate buffer solution with a pH value of 7 at a mass volume ratio (g / ml) of 1:1 to dilute the intestinal tract of the worm, and use an electric homogenizer to grind it into a homogenate under ice-bath conditions. Centrifuge at 20,000×g for 20 min at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of suspended solution, the lower layer of sedimentation, and the middle layer is recovered. The middle layer was centrifuged again at 20000×g at 4°C for 20 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.

[0066] Take part of the intestinal enzyme solution, and use the BCA method to detect the prote...

Embodiment 2

[0068] Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. Add 50mmol / L phosphate buffer solution with a pH value of 7 at a mass volume ratio (g / ml) of 1:1 to dilute the intestinal tract of the worm, and use an electric homogenizer to grind it into a homogenate under ice-bath conditions. Centrifuge at 18000×g for 30 min at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of suspended solution, the lower layer of sedimentation, and the middle layer is recovered. The middle layer was centrifuged again at 18000×g at 4°C for 30 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.

[0069] Take part of the intestinal enzyme solution, and use the BCA method to detect the protei...

Embodiment 3

[0071] Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. Add 50mmol / L phosphate buffer with a pH value of 7.2 at a mass-volume ratio (g / ml) of 1:2 to the parasite intestine for dilution, and use an electric homogenizer to grind into a homogenate under ice-bath conditions. Centrifuge at 25,000×g for 24 minutes at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of the suspended solution, the lower layer of sedimentation, and the recovery of the middle layer. The middle layer was centrifuged again at 25000×g at 4°C for 24 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.

[0072] Take part of the intestinal enzyme solution, and use the BCA method to detect the protein ...

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Abstract

The invention relates to the technical field of biomedicines, in particular to a queen bee intestinal enzyme, and a preparation method and application thereof. In the method, royal jelly polypeptides with molecular weights of between 1 and 3kDa and between 3 and 5kDa are obtained by extracting an intestinal enzyme from larvae of queen bees and simulating the enzymolysis environment in bodies of the queen bees to enzymolyze royal jellies. The prepared royal jelly polypeptides can be used for preparing an A beta inhibitor.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a queen bee intestinal enzyme, a preparation method and application thereof. Background technique [0002] In recent years, with the increase of the average life expectancy of human beings, the proportion of the elderly population has been increasing, and the aging of my country's population is becoming more and more obvious. Alzheimer's disease (AD), as the most common type of senile dementia, has caused the whole society to extensive attention. Alzheimer's disease is a complex multifactorial chronic progressive mental decline disease characterized by memory loss and cognitive dysfunction, clinical manifestations are progressive memory and cognitive decline, language barriers, psychomotor abnormalities and other symptoms . In the early stage of the disease, the near memory is mainly decreased, and the distant memory is also affected in the later stage of the disease, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/64C12P21/06
Inventor 冯成强孙平颜慧李虎臣
Owner BEIJING NORMAL UNIVERSITY
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