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A kind of bladder cancer quantitative detection test paper

A technology for detecting test strips and cancer quantification, which is applied in the field of quantitative detection test strips for bladder cancer, can solve the problems of bladder cancer that can only be qualitative but not quantitative, and achieves the effect of high accuracy and good specificity

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a quantitative detection test paper for bladder cancer markers, so as to overcome the defect that the existing bladder cancer detection can only be qualitative but not quantitative

Method used

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  • A kind of bladder cancer quantitative detection test paper
  • A kind of bladder cancer quantitative detection test paper
  • A kind of bladder cancer quantitative detection test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Prepare a colloidal gold solution with a particle size of 5-40nm by the classic Frens method: add 100mL of 0.01% HAuCl 4 Heat the solution to boiling, quickly add 0.5-5mL of sodium citrate solution under vigorous stirring, keep boiling for 5-30min, and then turn off the heat source to obtain a bright red transparent colloid.

[0034] 2. Select an appropriate pH value (5-10, preferably 7-9, specific values ​​such as: 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10), mix anti-uric fibronectin Monoclonal antibody and colloidal gold solution, the coating amount is 10-50μg / mL, and vortex reaction for 10-30 minutes, then add 10% PEG and 10% BSA to block, centrifuge to resuspend and concentrate to 1 / 100 of the original volume -1 / 10, a stable gold-labeled antibody was obtained.

[0035] 3. Preparation of the colloidal gold pad: select glass cellulose membrane as the colloidal gold pad material, redisperse the colloidal gold-FN antibody complex prepared above in 0.1mol / L Tris-HCl...

Embodiment 2

[0050] 1. Use normal human urine as the diluent for the FN antigen standard product to prepare serial concentration standard products: 10ng / mL, 500ng / mL, 800ng / mL, 1000ng / mL, 2000ng / mL, 3000ng / mL.

[0051] 2. Add a series of concentration standard products dropwise to the sample hole of the detection test paper, and after reacting for 10 minutes, read the ratio of the absorption optical density of the detection area to the quality control area through the gold standard immunochromatography analyzer, and use each concentration standard product separately 3 detection test papers were measured 3 times, averaged, the results are shown in Table 1, and corresponding standard curves were drawn (see figure 2 ).

[0052] 3. Utilize above-mentioned standard curve to detect clinical sample, calculate that the sensitivity of quantitative detection test paper of the present invention is 10ng / mL or below, the lower limit of linear range is lower than 10ng / mL and the upper limit is higher th...

Embodiment 3

[0056] Stability test of the test paper for quantitative detection of bladder cancer: the prepared test paper is sealed and packaged, and placed in a normal temperature (2-30° C.) and 37° C. environment for normal and accelerated tests.

[0057] After 30 days of the accelerated test, take out the detection test paper, measure the FN standard substance of 1000ng / ml, repeat the detection 20 times, CV≤5%, and the average value is between 990-1010ng / ml.

[0058] Test the test paper placed under normal temperature (2-30°C) according to the same method, investigate to the 26th month, measure the FN standard substance of 1000ng / ml, repeat the test 20 times, CV≤5%, and the average value is also controlled at Between 990-1010ng / ml. It shows that the test paper can be effectively stored for two years.

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Abstract

The invention relates to a test paper for quantitative detection of bladder cancer, which is composed of a sample pad, a colloidal gold pad, a nitrocellulose membrane, an absorbent paper and a bottom plate. On the nitrocellulose membrane, there is a detection area fixed with urinary fibronectin and sheep antibody. In the control area of ​​the mouse secondary antibody, there is a gold-labeled anti-urinary fibronectin antibody on the colloidal gold pad, wherein the gold-labeled antibody is dispersed on the colloidal gold pad after being dispersed in a pH 5-10 dilution. The sensitivity of quantitative detection is up to 10ng / mL, the linear range is from 10-3000ng / mL, and it has the advantages of strong specificity, high precision, good stability, easy operation, low cost, etc. It is suitable for primary screening and surgical treatment of bladder cancer. After tracking.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to a test paper for quantitative detection of bladder cancer. Background technique [0002] Bladder transitional cell carcinoma (BTCC) is the most common malignant tumor in the genitourinary system. Although surgical treatment can obtain good curative effect, the treatment cost is expensive and the postoperative recurrence rate is high. For a long time, cystoscopic pathological biopsy has been regarded as the gold standard for clinical diagnosis of bladder cancer, but it is an invasive operation, the diagnosis and treatment process is complicated, time-consuming and laborious, and the pain is unbearable for patients. Therefore, finding sensitive and specific bladder tumor markers and establishing a non-invasive, simple, rapid and cheap detection method have become research hotspots in the diagnosis and postoperative review of bladder cancer. [0003] Fibronectin (FN) is a macro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/532
Inventor 沈鹤柏赵露晶
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