A kind of fir tissue culture method

A tissue culture and culture method technology, applied in plant cells and other directions, can solve the problem of high price of Chinese fir tissue culture seedlings, and achieve the effect of simplifying tissue culture links and reducing tissue culture costs.

Inactive Publication Date: 2011-12-21
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

However, the complicated technical links in the traditional tissue culture process are all formulated around pollution prevention. Pollution c

Method used

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  • A kind of fir tissue culture method
  • A kind of fir tissue culture method

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Embodiment Construction

[0011] Prepare the culture medium under open conditions, adding bacteriostatic agents, but without high pressure. After the culture medium has solidified, it can be inoculated.

[0012] In the open tissue culture process of Chinese fir, the plant height, fresh weight, dry weight, number of roots, root length, and chlorophyll fluorescence parameters of the tissue cultured seedlings grown on the medium added with bacteriostatic agents were used as measurement indicators for further evaluation. Chinese fir open tissue culture method, the following conclusions are obtained:

[0013] (1) Buy mancozeb, bacteriobacillus and sodium hypochlorite directly from the store and make aqueous solutions with required concentrations. 62.5mg.L -1 ~78.13 mg.L -1 Mancozeb+60~80 mg.L -1 Bactericidal fruit + 3 ~ 6 mg.L -1 One or a combination of sodium hypochlorite has good antibacterial effect, the pollution rate of the combination is only 3.4% (see Table 1), and the maximum pollution rate doe...

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Abstract

The invention provides a Chinese fir tissue culture method. The culture method adopts open tissue culture, and the bacteriostatic agent used is one or more of Junsha Baoguo, mancozeb and sodium hypochlorite. The method of this invention can reduce the pollution rate to an acceptable range without affecting the effect of tissue culture. The tissue culture of plants in a bacterial environment fundamentally simplifies the tissue culture process and reduces the cost of tissue culture.

Description

technical field [0001] The invention relates to a fir tissue culture method. Background technique [0002] Chinese fir( Cunninghamia lanceolata ) is the most important fast-growing and high-yielding tree species in southern my country. It has excellent characteristics such as fast-growing, high-yielding, good material properties, and wide application. It has an important position in my country's plantation forests. Since Chinese fir is a cross-pollinated tree species, it is propagated by seeds, and the individual differentiation is large, so it is difficult to maintain the traits of the Chinese fir parent body; and conventional asexual reproduction methods such as cutting, layering or grafting are slow in breeding and difficult to meet the growing market demand. The tissue culture method is a rapid vegetative propagation technique. Selecting excellent Chinese fir clone materials and adopting the tissue culture method can not only maintain the excellent traits of the mot...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 林思祖丁国昌张艳林森
Owner FUJIAN AGRI & FORESTRY UNIV
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