A kind of preparation method of chloramphenicol residual freeze-dried powder standard sample in carp muscle

Abstract

The invention provides a preparation method for a standard sample of chloramphenicol residual matrix lyophiled powder in the muscle of a carp, which belongs to the technical field of animal food matrix standard substances in the residual detection of veterinary medicines. The problem that the bigger difference exists between the result of treatments such as extraction, purification and the like and a daily analytical sample as the condition of combining a target object with a matrix and a really detected sample are not consistent completely is solved. The method comprises the following steps of: carrying out cultivation and addition on a live carp by adopting chloramphenicol, so that the chloramphenicol is contained in carp in vivo, culturing the carp in water which does not contain a medicine until the medicine concentration in vivo reaches a stable metabolic state, fishing the carp, quickly freezing the carp to a temperature below -18 DEG C, unfreezing the frozen carp at a room temperature, taking a muscle part to homogenate to make into minced fillets, freezing, drying, screening and bottling the minced fillets, and encapsulating the minced fillets in a vacuum way, so as to prepare and obtain the standard sample of the chloramphenicol residual lyophiled powder in the muscle of the carp. The chloramphenicol concentration in the prepared standard sample is 1.0-10.0 [mu]g / kg.

Description

technical field [0001] The invention belongs to the technical field of animal food matrix standard substances for detection of veterinary drug residues, and in particular relates to a method for preparing standard samples of chloramphenicol residue matrix freeze-dried powder in carp muscle. Background technique [0002] Chloramphenicol (CAP), chemical name: D-(-)-sua-1-p-nitrophenyl-2-dichloroacetamido-1,3-propanediol. Molecular formula C 11 h 12 o 5 N 2 Cl 2 , molecular weight: 322.0124. [0003] Chloramphenicol is a synthetic broad-spectrum antibiotic that can cause human aplastic anemia, agranulocytosis, neonatal gray syndrome and other diseases, especially the low concentration of chloramphenicol drug residues can induce pathogenic bacteria drug resistance. The residue of chloramphenicol not only causes harm to the human body, but also seriously affects the export of animal food in my country. The main importing countries of aquatic products have different requir...

AI Technical Summary

Problems solved by technology

At present, the standard substances used for the detection of chloramphenicol residues are mainly pure chloramphenicol standard samples, and the detection quality control is mainly performed by adding pure chloramphenicol standard substances to the samples for detection and analysis. This analysis method cannot represent the target The state in which the analyte actually exists in vivo, that is, the "internalized" state that cannot represent the target analyte

The standard sample obtained in this way is not a real physical standard sample. Because the combination of the target object and the matrix is ​​not completely consistent with the real test sample, the results of extraction, purification, etc. are quite different from the daily analysis samples, thus affecting the accuracy of the results. Reliability and Validity

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