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Method for measuring sulglicotide content

A determination method and thioglycopeptide technology are applied in the field of determination of thioglycopeptide content to achieve the effect of good specificity and accuracy

Active Publication Date: 2012-01-04
SHANDONG TOPSCI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In foreign countries, only a few countries such as South Korea and Italy have conducted research on the determination method of sulfglucopeptide content, but there are no relevant articles or patent publications, and it is still a blank in China.

Method used

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  • Method for measuring sulglicotide content
  • Method for measuring sulglicotide content
  • Method for measuring sulglicotide content

Examples

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Effect test

Embodiment 1

[0026] A method for assaying sulfglycopeptide content is characterized in that it comprises the following steps:

[0027] (1) Take the sulfglucopeptide test substance and standard substance, add water and dissolve them respectively to form 10 mg / mL test substance solution and standard substance solution;

[0028] (2) Use a high-performance liquid chromatograph, use Dion’s AS11 anion exchange column, fill the column with ethylvinylbenzene-divinylbenzene resin with alkanol quaternary ammonium as the functional group, and the filling volume is 2mm×250mm, There is an AG11 anion guard column in front of the chromatographic column, and the filling volume is 2mm×50mm;

[0029] Using 0.04wt% sodium dihydrogen phosphate solution with a pH value of 3.0 as mobile phase A and sodium perchlorate-phosphate solution with a pH value of 3.0 as mobile phase B, perform linear gradient washing at a flow rate of 0.22 mL / min The detection wavelength is 205nm, and the injection volume is 10 μ L; th...

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Abstract

The invention relates to the technical field of sulglicotide serving as a chemical medicament and a method for measuring sulglicotide content. The method comprises the following steps of: preparing 10 mg / ml of specimen solution and 10 mg / ml of standard substance solution; filling ethyl vinyl benzene-divinylbenzene resin which takes alkanol quaternary ammonium as a functional group into a chromatographic column of a high performance liquid chromatograph, and performing linear gradient washing by taking 0.04 weight percent of sodium dihydrogen phosphate solution with the pH value of 3.0 as a mobile phase A and sodium perchlorate-phosphate solution with the pH value of 3.0 as a mobile phase B at the flow speed of 0.22 milliliter / minute, wherein the detection wavelength is 205 nanometers, and the sample injection amount is 10 microliters; and recording a chromatogram map, and measuring the sulglicotide content of specimens. By the method, the retention time is advanced to about 19 minutes, a peak shape is also improved greatly, and the detection sensitivity is improved, so the method has high specificity and accuracy.

Description

technical field [0001] The invention relates to the technical field of chemical medicine sulfoglycopeptide, in particular to a method for determining the content of sulfoglycopeptide. Background technique [0002] Suglucopeptide has a unique curative effect on peptic ulcer, but due to the shortage of its raw materials, most countries can only rely on imports, and research in this area has just started in my country. Therefore, on the basis of optimizing the synthesis process of sulfoglycopeptide, quality research has become the main direction of sulfoglycopeptide research. The determination of content is an important aspect of quality research, but the determination of content is a blank at home and abroad at present, so it is particularly urgent to establish a content determination method with good specificity and accuracy. In foreign countries, only a few countries such as South Korea and Italy have conducted research on the determination method of sulfglucopeptide cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 王朋田李娟孙廷华
Owner SHANDONG TOPSCI BIO TECH
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