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Method for positioning capillary tube array by using raman spectral imaging

A capillary array and Raman spectroscopy technology is applied in the field of locating the imaging position of the capillary array on the charge-coupled element, which can solve the problem of not eliminating mature technologies, and achieve the effects of improving analysis accuracy, eliminating errors, and being easy to operate.

Active Publication Date: 2012-01-25
THE FIRST RES INST OF MIN OF PUBLIC SECURITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for positioning capillary tube array by using raman spectral imaging
  • Method for positioning capillary tube array by using raman spectral imaging
  • Method for positioning capillary tube array by using raman spectral imaging

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Embodiment Construction

[0030] The method of the present invention will now be described in further detail in conjunction with the accompanying drawings and embodiments.

[0031] figure 1 It is a schematic diagram of the operation of the method of the present invention. The laser beam 1 is irradiated at the detection window of the capillary bundle 2, the laser is scattered by the quartz of the single capillary 3 or the water in the solution in the tube, and emits Raman light 4, passes through the light-taking slit, the grating splits the light, and the lens is focused and imaged on the CCD 5, analyze the spectrogram on the CCD 5 to locate the position of the capillary bundle.

[0032] Position positioning technology uses dual laser beams, 488nm and 514.5nm respectively. The characteristic Raman peaks of 488nm and 514.5nm lasers scattered by water can be projected onto the data acquisition CCD to obtain image 3 . The blue light (left) is the collected Raman light intensity of 488nm laser scatter...

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Abstract

The invention provides a method for positioning a capillary tube array on a charge coupled device by using a raman spectrum. The method comprises the following steps of: (1) irradiating a capillary tube bundle by using laser to emit raman light; (2) imaging on the charge coupled device (CCD) through a raman imager; and (3) analyzing a spectrogram on the CCD to position each capillary tube in the capillary tube bundle. In the method, the raman spectrum of quartz or water is excited by using double-beam laser to realize spectral imaging; and thus, positioning and parallelism adjustment of the capillary tube on the CCD are realized, error during trace reagent analysis of the capillary tube array can be eliminated, and the analysis accuracy is improved.

Description

technical field [0001] The invention relates to a method for locating the imaging position of a capillary array on a charge-coupled element, and belongs to the technical field of high-throughput microanalysis. Background technique [0002] Because the capillary has good heat dissipation performance, high withstand voltage and small injection volume, it has the most basic and excellent characteristics such as high efficiency, fast speed and less sample consumption required by instrumental analysis. The voltage applied to both ends of the capillary can be as high as 30kV, and the longitudinal electric field strength of the separation capillary can reach more than 400V / cm, so the separation operation can achieve a very high separation efficiency in a short period of time (the number of theoretical plates can reach 400,000 / m above, up to 107 / m order of magnitude). Because the inner diameter of the capillary is very small (generally <100 μm), for a capillary with an inner dia...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 李彬张涛贾二惠赵怡鹤陈学亮
Owner THE FIRST RES INST OF MIN OF PUBLIC SECURITY