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Lipopeptide biosurfactant, preparation method thereof, and application thereof

A biological surface and active agent technology, applied in biochemical equipment and methods, microbial-based methods, microorganisms, etc., can solve problems such as insufficient production, high price, and limitations, and achieve improved recovery, good synergistic effects, and reduced The effect of moisture content

Inactive Publication Date: 2012-02-15
CHINA NAT OFFSHORE OIL CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large amount of chemical surfactants used in the ternary system and the high price of qualified products in the market, insufficient output, and environmental problems, the further promotion and application of this technology is greatly restricted.

Method used

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  • Lipopeptide biosurfactant, preparation method thereof, and application thereof
  • Lipopeptide biosurfactant, preparation method thereof, and application thereof
  • Lipopeptide biosurfactant, preparation method thereof, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Preparation of lipopeptide biosurfactant

[0036] Method I

[0037] 1. Preparation of lipopeptide biosurfactant

[0038] Fermentation to produce lipopeptide biosurfactants with Bacillus subtilis ZW-3 (Bacillus subtilis ATCC 21770) (purchased from the American Model Collection Center (ATCC)) includes the following steps:

[0039] (1) Seed culture: Pick Bacillus subtilis ZW-3 from the slant medium, inoculate it in the seed medium, and cultivate at 37°C and 250r / min for 11 hours with shaking to obtain a seed culture for fermentation Medium inoculation.

[0040] The seed culture medium consists of the following components:

[0041] Glucose, beef extract, yeast powder, peptone, MgSO 4 ·7H 2 O and water;

[0042] The concentrations of the above components in the seed culture medium are:

[0043] Glucose 0.05g / L; beef extract 0.03g / L; yeast powder 0.03g / L; peptone 0.1g / L; MgSO 4 ·7H 2 O0.02g / L;

[0044] The pH of the seed medium is 7.5.

[0045] (2) Fermentation culture: The seed...

Embodiment 2

[0093] Example 2. Application of lipopeptide biosurfactant in ternary compound flooding

[0094] 1. Preparation of composition containing lipopeptide biosurfactant and alkylbenzene sulfonate

[0095] 1. Determination of the ratio of lipopeptide biosurfactant and alkylbenzene sulfonate

[0096] According to the characteristics of the ratio of high surfactant concentration-high alkali concentration, low surfactant concentration-low alkali concentration in the ternary compound flooding, the fixed NaOH concentration is 0.6wt%~0.8wt% to determine the lipopeptide biosurfactant and The ratio of alkylbenzene sulfonate. The lipopeptide biosurfactant prepared in Example 1 (the lipopeptide concentration is 0.084% by weight) and the aqueous solution of alkylbenzene sulfonate (the concentration is 50.0% by weight) are respectively 1:3, 2:3, The ratios of 1:1, 3:2 and 3:1 are formulated into mixed solutions of active substances with different concentrations. The mass ratios of lipopeptide biosu...

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Abstract

The invention discloses a lipopeptide biosurfactant, a preparation method thereof, and an application thereof. The method for preparing the lipopeptide biosurfactant comprises steps that: bacillus subtilis is fermented, such that a fermentation broth is obtained; and the lipopeptide biosurfactant is obtained. When the lipopeptide biosurfactant is formulated with other surfactants, a good synergic effect is provided. As a result of a core oil-displacement experiment employing an alkali-surfactant-polymer flooding complex system of the lipopeptide biosurfactant, water content can be greatly reduced, and recovery efficiency can be improved.

Description

Technical field [0001] The invention relates to a lipopeptide biosurfactant and its preparation method and application. Background technique [0002] With the reduction of new crude oil reserves discovery opportunities and the declining production of oilfields in the middle and late stages of exploitation, the development of technologies for enhancing oil recovery has been promoted, among which ASP flooding technology is becoming one of the main technologies for tertiary oil recovery in the future. ASP (alkali-surfactant-polymer flooding) is made up of alkali, surfactant and polymer in a certain proportion. Daqing Oilfield has successfully carried out three ASP pilot field tests and two expanded field tests, and the recovery rate has increased by more than 20% compared with water flooding. At present, two pilot field tests have been carried out, and good results have been seen. Effect. Due to the large amount of chemical surfactants in the ternary system, the high price of qual...

Claims

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Application Information

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IPC IPC(8): C09K8/584C12P21/00C12R1/125
Inventor 王大威
Owner CHINA NAT OFFSHORE OIL CORP
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