High-temperature-resistant citric acid producing strain

A technology for producing strains and high-yielding strains, applied in the field of bioengineering, can solve the problems of few methods for improving the heat resistance of strains, and achieve the effects of broad prospects for industrial application, lower production costs, and lower costs.

Inactive Publication Date: 2012-02-15
NANJING UNIV OF TECH
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Problems solved by technology

[0003] Breeding of bacterial strains is a key technology in the fermentation industry, and the breeding of citric acid producing strains in the domestic prior

Method used

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  • High-temperature-resistant citric acid producing strain
  • High-temperature-resistant citric acid producing strain
  • High-temperature-resistant citric acid producing strain

Examples

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Embodiment 1

[0031] Description of this example Aspergillus niger Mutagenesis and screening methods for zjs-8.

[0032] Starting strain: Aspergillus niger purchased from China Industrial Microorganism Collection Center (CICC) Aspergillus niger CICC NO: 40567 is the starting strain.

[0033] 1. Ion beam mutagenesis.

[0034] Take a slant of mature spores of the starting strain of citrate-producing Aspergillus niger that has been cultured for 5-6 days, wash the spores with sterile water, filter them through gauze, and place them in a pre-sterilized Erlenmeyer flask with glass beads. Shake on the bed for 30-60 minutes until microscopic examination reveals dispersed monospores. Count by hemocytometer and adjust the spore concentration to 1×10 with sterile water 7 Individuals / mL is the single spore suspension. Take 0.1 mL of the spore suspension, evenly spread it on the surface of a sterile petri dish, and air-dry it at room temperature under sterile conditions. Then put the petri ...

Embodiment 2

[0050] This example illustrates the use of Aspergillus niger zjs-8 is a small test method for producing citric acid by high-temperature fermentation on a 5 L tank.

[0051] The spores were directly inoculated into 2.5 L seed tank culture medium with an inoculum amount of 350,000 / mL, cultured at a temperature of 40°C, an aeration ratio of 0.8 vvm, and a stirring speed of 800 rpm. After the seeds matured (pH ≤2.5) Put 10% of the inoculum into a fermenter with 3 L of fermentation medium, ferment at a temperature of 40 or 42°C, other conditions are the same as the seed tank, and ferment until the amount of reducing sugar in the fermentation broth is low After 0.3%, it was released into the tank. Through the 5 L tank test, it was found that zjs-8 is a strain that can produce acid stably at a high temperature of 42 °C and has a greater increase than the original strain. The cycle of the seed tank is 22 hours, which is 4 hours shorter than that of the starting strain; the cycle of...

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Abstract

The invention belongs to the technical field of biological engineering, and relates to a high-temperature-resistant citric acid producing strain. The invention also relates to a mutagenesis screening method and an application method of the strain. The strain provided by the invention is classified and named as Aspergillus niger zjs-8 with the conservation number of CCTCC (China Center for Type Culture Collection) NO: M2011359. The method for obtaining the strain comprises: mutagenizing a starting strain by ion beam implantation, then coating the treated strain liquid on a screening flat plate containing bromocresol green, culturing at a high temperature, selecting single colony with a larger ratio of color changing circle to the colony diameter, and carrying out high-temperature fermentation to produce citric acid. The strain screened by the method can bear 42 DEG C high temperature, and has stable genetic character and higher acid producing level under the high-temperature condition, thereby producing a feasible way for energy-saving production of citric acid industry.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to a high-temperature resistant citric acid production strain. The invention also relates to a mutagenesis screening method and application method for obtaining the strain. Background technique [0002] The citric acid industry is a high-energy-consuming industry. The citric acid fermentation process is a heat-generating process, which requires a large amount of circulating cooling water to maintain the fermentation temperature. In China, Aspergillus niger ( Aspergillus niger ), the fermentation temperature of this strain is currently around 37.5°C. According to the actual calculation of the factory, if the fermentation temperature can be increased by 1°C, the amount of circulating cooling water in winter will be reduced by 15.2 tons / ton of citric acid (both are 300 m 3 Fermentation tank), in summer it is reduced to 31.2 tons / ton of citric acid. The reduction of circulating ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/01C12P7/48C12R1/685
Inventor 黄和李霜左静徐晴满云
Owner NANJING UNIV OF TECH
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