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Seminal plasma gamma-L-glutamyl transpeptidase activity quantitative detecting reagent kit and application thereof

A technology for quantitative detection of glutamyl transpeptidase, which is applied in the field of biomedical testing and determination to achieve the effects of simplified operation steps, stable and reliable detection results and strong affinity

Inactive Publication Date: 2012-03-21
南京欣迪生物药业工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regrettably, as of now, no matter whether it is foreign or domestic, for the detection of seminal plasma γ-L-glutamyl transpeptidase, except for the early scientific research results, there is no one that can be matched with an automatic biochemical analyzer Therefore, it is particularly urgent and important to establish a seminal plasma γ-L-glutamyl transpeptidase activity quantitative detection kit and detection method for use with an automatic biochemical analyzer.

Method used

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  • Seminal plasma gamma-L-glutamyl transpeptidase activity quantitative detecting reagent kit and application thereof
  • Seminal plasma gamma-L-glutamyl transpeptidase activity quantitative detecting reagent kit and application thereof
  • Seminal plasma gamma-L-glutamyl transpeptidase activity quantitative detecting reagent kit and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A seminal plasma γ-L-glutamyl transpeptidase activity quantitative detection kit in this example is a double reagent, including R1 reagent and R2 reagent, prepared according to the following components and dosage respectively:

[0042] 1) R1 reagent

[0043] pH8.2 Tris buffer 180mmol / L;

[0044] Glycyl peptide 100mmol / L;

[0045] Sodium azide 0.05%;

[0046] 2) R2 reagent

[0047] pH8.2 Tris buffer 180mmol / L;

[0048] L-γ-glutamyl-p-nitroaniline 1.25mmol / L;

[0049] Thimerosal 0.02%;

[0050] After the above reagents are completely dissolved, they are divided into bottles to make liquid double reagents and stored at 2-8°C for later use.

Embodiment 2

[0052] A seminal plasma γ-L-glutamyl transpeptidase activity quantitative detection kit in this example is a double reagent, including R1 reagent and R2 reagent, prepared according to the following components and dosage respectively:

[0053] 1) R1 reagent

[0054] pH8.3 Tris buffer 150mmol / L;

[0055] Glycine 90mmol / L;

[0056] ProClin300 0.02%;

[0057] 2) R2 reagent

[0058] pH8.3 Tris buffer 150mmol / L;

[0059] L-γ-glutamyl-3-carboxy-4-nitroaniline 0.6mmol / L;

[0060] Thimerosal 0.01%;

[0061] After the above reagents are completely dissolved, they are divided into bottles to make liquid double reagents and stored at 2-8°C for later use.

Embodiment 3

[0063] A seminal plasma γ-L-glutamyl transpeptidase activity quantitative detection kit in this example is a double reagent, including R1 reagent and R2 reagent, prepared according to the following components and dosage respectively:

[0064] 1) R1 reagent

[0065] pH8.1 Tris buffer 100mmol / L;

[0066] Glycyl peptide 100mmol / L;

[0067] ProClin150 0.1%;

[0068] 2) R2 reagent

[0069] pH8.1 Tris buffer 100mmol / L;

[0070] L-γ-glutamyl-p-nitroaniline 3.0mmol / L;

[0071] ProClin300 0.05%;

[0072] After the above reagents are completely dissolved, they are divided into bottles to make liquid double reagents and stored at 2-8°C for later use.

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Abstract

The invention discloses a seminal plasma gamma-L-glutamyl transpeptidase activity quantitative detecting reagent kit, which comprises a reagent R1 and a reagent R2; the reagent R1 comprises the following ingredients: 100 to 200 mmol / L trihydroxymethyl aminomethane buffer with pH value of 8.0 to 8.3, 90 to 110mmol / L glycylglycine and 0.01%to 0.1% antiseptic; and the reagent R2 comprises the following ingredients: 100 to 200mmol / L trihydroxymethyl aminomethane buffer with pH value of 8.0 to 8.3, 0.5 to 3.0mmol / L substrate and 0.01% to 0.1% antiseptic. The two liquid reagents can be directly used without being frozen at the temperature of minus 20 DEG C, the type and the consumption of the reagent can be remarkably reduced, and the sample is free from being diluted for more than 200 times; the substrate has good specificity and good appetency to the enzyme, so the reaction speed is fast; and the reagent kit is matched with an automatic biochemical instrument to use, so the procedures can be implified, the reagent can be saved, the manual error can be reduced, automatic and large-scale detection can be realized, the detection result is more accurate and more reliable, and the detection efficiency can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of biomedical inspection and measurement, and relates to a kit for in vitro diagnosis and its application, in particular to a seminal plasma γ-L-glutamyl transpeptidase activity quantitative detection kit and its application. Background technique [0002] Seminal plasma γ-L-glutamyl transpeptidase (abbreviated as γ-L-glutamyl transpeptidase) is mainly derived from the prostate, and its content is about 200-500 times that in serum. The detection of its activity can be used to evaluate the prostate In addition, seminal plasma γ-L-glutamyl transpeptidase is a key enzyme of glutathione metabolism and also plays a very important role in protecting sperm from oxygen free radical damage. In patients with low prostate function, the activity of γ-L-glutamyl transpeptidase decreased significantly; in patients with prostate cancer and benign prostatic hyperplasia, its activity increased significantly. [0003] At pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 高建陆金春陈晓慧
Owner 南京欣迪生物药业工程有限责任公司
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