Spathiphyllum somatic embryo inducing and plant regenerating method
A technology for somatic embryos and plant regeneration, which is applied in the fields of botanical equipment and methods, plant regeneration, and horticultural methods. Embryos and regenerated plants, etc., to achieve the effects of improved induction efficiency, easy access, and convenient materials
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Example Embodiment
[0019] Embodiment 1: This embodiment is carried out through the following steps:
[0020] (1) Somatic embryo induction. Take the Heliconia S. cannifolium After sterilization, the stems or apical buds are inoculated into the clumping bud induction medium to obtain a sterile test-tube plantlet, and then transferred to the test-tube plantlet proliferation medium at a temperature of 24 to 30 ℃ and a light intensity of 1000 to 1400 Lux, in an environment of 10 to 14 hours of light a day, subculture once every 3-4 weeks, and the test tube plantlets continue to proliferate. Cut the leaves of the young test tube plantlets into 1-10 mm wide pieces, inoculate them in the somatic embryo induction medium, and culture them in a dark environment at a temperature of 24-30 ℃ for 2 to 4 weeks to obtain somatic embryos;
[0021] (2) Proliferation of somatic embryos: subculture the obtained somatic embryos in a somatic embryo induction medium. After culturing in a dark environment at a temperature...
Example Embodiment
[0025] Example 2: The difference between this example and Example 1 is that in step (1), the petiole of the test tube plantlet is cut into 1-10 mm sections, and then inoculated in a somatic embryo induction medium for culture to obtain somatic embryos; Example 1 is the same.
Example Embodiment
[0026] Example 3: The difference between this example and Example 1 or 2 is that the somatic embryo induction medium in step (1) uses MS medium as the basic medium, and each 1 liter of medium contains 0.05 to 0.25 mg of thiol Phenuron, 0.5-2.0 mg of 2,4-dichlorophenoxyacetic acid, 20-40 g of sucrose and 6-9 g of agar, pH value 5.6-6.0; in step (2) the somatic embryo proliferation medium MS medium is used as the basic medium, and every 1 liter of medium contains 0.05~0.25 mg of thidiazuron, 0.5~1.0 mg of 2,4-dichlorophenoxyacetic acid, 20~40 grams of sucrose and 6~ 9 grams of agar has a pH of 5.6 to 6.0; the rest is the same as in Example 1 or 2.
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