Spathiphyllum somatic embryo inducing and plant regenerating method

A technology for somatic embryos and plant regeneration, which is applied in the fields of botanical equipment and methods, plant regeneration, and horticultural methods. Embryos and regenerated plants, etc., to achieve the effects of improved induction efficiency, easy access, and convenient materials

Inactive Publication Date: 2012-03-28
广东省农业科学院花卉研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, the efficiency of inducing somatic embryos is relatively low. Generally, only a single or a dozen somatic embryos can be produced on each explant; the collection of explants also needs to be in the flowering seas

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Embodiment 1: This embodiment is carried out through the following steps:

[0020] (1) Somatic embryo induction. Take the Heliconia S. cannifolium After sterilization, the stems or apical buds are inoculated into the clumping bud induction medium to obtain a sterile test-tube plantlet, and then transferred to the test-tube plantlet proliferation medium at a temperature of 24 to 30 ℃ and a light intensity of 1000 to 1400 Lux, in an environment of 10 to 14 hours of light a day, subculture once every 3-4 weeks, and the test tube plantlets continue to proliferate. Cut the leaves of the young test tube plantlets into 1-10 mm wide pieces, inoculate them in the somatic embryo induction medium, and culture them in a dark environment at a temperature of 24-30 ℃ for 2 to 4 weeks to obtain somatic embryos;

[0021] (2) Proliferation of somatic embryos: subculture the obtained somatic embryos in a somatic embryo induction medium. After culturing in a dark environment at a temperature...

Example Embodiment

[0025] Example 2: The difference between this example and Example 1 is that in step (1), the petiole of the test tube plantlet is cut into 1-10 mm sections, and then inoculated in a somatic embryo induction medium for culture to obtain somatic embryos; Example 1 is the same.

Example Embodiment

[0026] Example 3: The difference between this example and Example 1 or 2 is that the somatic embryo induction medium in step (1) uses MS medium as the basic medium, and each 1 liter of medium contains 0.05 to 0.25 mg of thiol Phenuron, 0.5-2.0 mg of 2,4-dichlorophenoxyacetic acid, 20-40 g of sucrose and 6-9 g of agar, pH value 5.6-6.0; in step (2) the somatic embryo proliferation medium MS medium is used as the basic medium, and every 1 liter of medium contains 0.05~0.25 mg of thidiazuron, 0.5~1.0 mg of 2,4-dichlorophenoxyacetic acid, 20~40 grams of sucrose and 6~ 9 grams of agar has a pH of 5.6 to 6.0; the rest is the same as in Example 1 or 2.

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PUM

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Abstract

The invention provides a spathiphyllum somatic embryo inducing and plant regenerating method, which relates to a somatic embryo inducing and plant regenerating method and comprises the following steps of: 1, taking young and tender spathiphyllum test tube seedlings, cutting blades or petioles into small sections to be induced, and obtaining somatic embryos; 2, subculturing the somatic embryos into a somatic embryo multiplication culture medium for mass multiplication; and 3, transferring the somatic embryos onto the plant regeneration culture medium for culture to obtain complete small plants. The method has the advantages that the somatic embryo inducing rate reaches 97 percent to 100 percent, 50 to 200 somatic embryos can be formed on the surface of an explant in the primary culture, the inducing efficiency is obviously improved, and the plant regeneration rate of the obtained somatic embryos reaches 85 to 95 percent. The method solves the problems that the existing spathiphyllum somatic embryo has low inducing efficiency, and the explant material collection is inconvenient.

Description

[0001] technical field [0002] The invention relates to the technical field of plant induced regeneration, in particular to a method for inducing and regenerating plants from somatic embryos of Heliconia chinensis. Background technique [0003] White crane taro ( Spathiphyllum Schott), also known as white palm or smooth sailing, is an important tropical ornamental plant native to Central and South America, and is very popular in European and American markets. Introduced to mainland my country in the 1980s, it rapidly developed into an industry and became one of the most important tropical ornamental plants in my country. [0004] The plant tissue culture of Heliconia chinensis has been reported since the 1970s. It mainly uses explants such as terminal buds and stem segments to induce clustered buds, expands the number of reproduction by clustering buds, and then obtains complete plants by rooting culture. Its purpose is to achieve in vitro rapid propagation, expand the nu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 于波刘金梅刘晓荣廖飞雄
Owner 广东省农业科学院花卉研究所
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