Determination reagent for eliminating interference of ascorbic acid by uricase method and application method thereof

A technology of ascorbic acid and uricase, applied in the biological field, to achieve the effect of excellent interference performance, good stability, and strong anti-interference performance

Inactive Publication Date: 2012-04-18
ZHEJIANG CENTURY CONDOR MEDICAL SCI & TECHNOLOTY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Determination reagent for eliminating interference of ascorbic acid by uricase method and application method thereof
  • Determination reagent for eliminating interference of ascorbic acid by uricase method and application method thereof
  • Determination reagent for eliminating interference of ascorbic acid by uricase method and application method thereof

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Embodiment 1

[0041] (1) Sample preparation: the sample is fasting non-hemolyzed serum or plasma (heparin anticoagulation, 0.1mg heparin can anticoagulate 1.0ml blood; EDTA anticoagulation: 1.8mg EDTA can anticoagulate 1.0ml blood). Samples should be transported and stored at low temperature. Uric acid in samples is stable for 8 hours at room temperature, 3 days at 2°C to 8°C, and 6 months at -20°C.

[0042] (2) Prepare the uric acid determination reagent according to the following ingredients and dosage:

[0043] Reagent 1:

[0044] Phosphate buffer (pH 6.0-8.0) 0.10-100g / L,

[0045] Peroxidase 0.10-10KU / L,

[0046] N-ethyl-N-(3-toluene)-N-succinylethylenediamide EMSE 0.10-10g / L,

[0047] Anti-interference enzyme 0.10-10KU / L,

[0048] Stabilizer 0.10-100g / L;

[0049] Reagent 2:

[0050] Phosphate buffer (pH 6.0-8.0) 0.10-100g / L,

[0051] Peroxidase 0.10-10KU / L,

[0052] Uricase 0.10-10KU / L,

[0053] 4-Aminoantipyrine 4-AAP 0.10-10g / L,

[0054] Stabilizer 0.10-100g / L.

[0055]The...

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Abstract

The invention provides a determination reagent for eliminating interference of ascorbic acid (vitamin C, VC) by uricase method and an application method thereof. The reagent disclosed herein comprises liquid double reagents, and has good ascorbic acid interference resistance and excellent stability. According to the invention, a two point terminal method is adopted, and the reagent can be applied in semi/full-automatic biochemistry analyzers; the reagent is prepared by using a specific enzyme, thus the reagent has the characteristics of high sensitivity and strong interference resistance; and by adding the selected stabilizing agent, the reagent has good stability, and the reagent can be stored at 2-8 DEG C (in sealed dark place)for 12 months without influence on the performances. The reagent can be widely applied for diagnosis of acute and chronic nephritis, renal calculus, gout and other kidney diseases and leukemia, multiple myeloma and other diseases caused by excessive metabolism of nucleic acid, health check, general examination of kidney diseases and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a uricase assay reagent for eliminating ascorbic acid interference and a use method thereof. Background technique [0002] Uric acid (UA) is the final product of purine metabolism in nucleic acid. In the human body, purine nucleotides are decomposed to generate purine nucleosides and purines, which are deaminated and oxidized by hydrolysis to finally generate UA. UA is excreted with urine, and all UA in the blood is filtered out by the glomeruli and almost completely reabsorbed in the renal tubules, so the clearance rate of UA is extremely low. The UA excreted by the kidneys accounts for 2 / 3-3 / 4 of the total daily excretion, and the rest is decomposed by microbial enzymes in the gastrointestinal tract. When GFR is reduced, UA cannot be excreted normally, and the concentration of UA in blood increases. Some drugs also affect the excretion of UA, such as thiazide diuretics and hydroxys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/62C12Q1/28C12Q1/26
Inventor 王浩
Owner ZHEJIANG CENTURY CONDOR MEDICAL SCI & TECHNOLOTY CORP
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