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Model for arterial thrombosis for rabbit by improved thread-drawing method

A hanging-wire method and arterial technology, which is applied in the field of improved rabbit arterial thrombosis model by hanging-wire method, can solve the problems that the temperature is not affected by the surrounding tissue and the stability is not good.

Inactive Publication Date: 2012-05-02
王东生 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical methods such as trypsin thrombosis method, hydrogen peroxide method, carrageenan, ferric chloride thrombosis method, etc., are limited due to the need to perfuse certain chemical substances. Physical methods such as current injury method require constant current, The blood vessel temperature is not affected by the surrounding tissue, and the arterial short circuit method requires a silicone tube, which has certain limitations
The hanging-wire thrombus model is one of the commonly used models to understand the fate of emboli in the body and evaluate the efficacy of thrombolytic drugs as a whole. It is simple, inexpensive, intuitive and convenient. Reversible, poor stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0004] Rabbits (normal-grade healthy male rabbits, weighing about 2kg) were anesthetized with 25% hexyl carbamate (urethane) (4mL / kg, administered through the ear vein), cut the skin on the right side of the neck, and separated the subcutaneous The tissue and muscle layer fully exposed the vascular and nerve bundles, freed the common carotid artery, and clamped the proximal end and the distal end of the common carotid artery with microvascular clips, leaving an artery length of about 4 cm between the two ends. Use a 12-gauge fine sewing needle to puncture the blood vessel at the proximal end, grasp a certain strength, and accurately advance 3 cm in the blood vessel along the direction of the artery, and pass it through the distal end. ml of collagen I for 4 hours) into the blood vessel, the needle was coated with surgical glue, and the arterial clamp at the proximal end was opened after 10 minutes.

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PUM

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Abstract

The invention discloses a model for arterial thrombosis for animals, which uses a method that collagen coats the thread to copy, wherein collagen induced platelets are gathered irreversibly and consistent with thrombosis in human body. The method comprises the steps of: penetrating the material at the proximal end into the blood vessel at the end by a No.12 fine needle; mastering the dynamics; propelling the fine needle accurately for 3cm in the blood vessel along arterial trend; punching out from the distal end; guiding a No.1 silk thread into the blood vessel and coating the pinhole by surgical glue; and opening the artery clamp at the proximal end 10 minutes later. Compared with common thread-drawing method as an evaluating control group, the improved thread-drawing method provided by the invention is remarkably superior to the common thread-drawing method in the aspects of morphology, FDP, D-dimer, tissue factor and the like. The model for arterial thrombosis for rabbit by the improved thread-drawing method is intuitive, reliable, good in repeatability and high in success rate, can reflect the effect of antithrombotic medicines and is an ideal model for arterial thrombosis for animals.

Description

Technical field: [0001] Cardiovascular and cerebrovascular diseases based on arterial thrombosis are common and frequently-occurring diseases, and the incidence rate is increasing year by year, accounting for the first place in the mortality rate in my country. Research on the mechanism of thrombus formation, diagnosis of thrombus, and screening of antithrombotic drugs has attracted great attention, among which the establishment of thrombus models has become a key technology in the study of thrombus formation mechanism. Background technique: [0002] Animal models of thrombosis in vivo can be roughly divided into three categories: arterial, venous, and microvascular thrombosis. Among them, arterial thrombosis is the most common. Endothelial injury and platelet adhesion are the initiating factors of arterial thrombosis. The methods for inducing thrombus formation are mainly physical and chemical. Chemical methods such as trypsin thrombosis method, hydrogen peroxide method, c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A01K67/027
Inventor 王东生钟广伟王菡陈方平贺石林魏星田丽君龚志军
Owner 王东生
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