Model for arterial thrombosis for rabbit by improved thread-drawing method
A hanging-wire method and arterial technology, which is applied in the field of improved rabbit arterial thrombosis model by hanging-wire method, can solve the problems that the temperature is not affected by the surrounding tissue and the stability is not good.
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[0004] Rabbits (normal-grade healthy male rabbits, weighing about 2kg) were anesthetized with 25% hexyl carbamate (urethane) (4mL / kg, administered through the ear vein), cut the skin on the right side of the neck, and separated the subcutaneous The tissue and muscle layer fully exposed the vascular and nerve bundles, freed the common carotid artery, and clamped the proximal end and the distal end of the common carotid artery with microvascular clips, leaving an artery length of about 4 cm between the two ends. Use a 12-gauge fine sewing needle to puncture the blood vessel at the proximal end, grasp a certain strength, and accurately advance 3 cm in the blood vessel along the direction of the artery, and pass it through the distal end. ml of collagen I for 4 hours) into the blood vessel, the needle was coated with surgical glue, and the arterial clamp at the proximal end was opened after 10 minutes.
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