Model for arterial thrombosis for rabbit by improved thread-drawing method

Abstract

The invention discloses a model for arterial thrombosis for animals, which uses a method that collagen coats the thread to copy, wherein collagen induced platelets are gathered irreversibly and consistent with thrombosis in human body. The method comprises the steps of: penetrating the material at the proximal end into the blood vessel at the end by a No.12 fine needle; mastering the dynamics; propelling the fine needle accurately for 3cm in the blood vessel along arterial trend; punching out from the distal end; guiding a No.1 silk thread into the blood vessel and coating the pinhole by surgical glue; and opening the artery clamp at the proximal end 10 minutes later. Compared with common thread-drawing method as an evaluating control group, the improved thread-drawing method provided by the invention is remarkably superior to the common thread-drawing method in the aspects of morphology, FDP, D-dimer, tissue factor and the like. The model for arterial thrombosis for rabbit by the improved thread-drawing method is intuitive, reliable, good in repeatability and high in success rate, can reflect the effect of antithrombotic medicines and is an ideal model for arterial thrombosis for animals.

Description

Technical field: [0001] Cardiovascular and cerebrovascular diseases based on arterial thrombosis are common and frequently-occurring diseases, and the incidence rate is increasing year by year, accounting for the first place in the mortality rate in my country. Research on the mechanism of thrombus formation, diagnosis of thrombus, and screening of antithrombotic drugs has attracted great attention, among which the establishment of thrombus models has become a key technology in the study of thrombus formation mechanism. Background technique: [0002] Animal models of thrombosis in vivo can be roughly divided into three categories: arterial, venous, and microvascular thrombosis. Among them, arterial thrombosis is the most common. Endothelial injury and platelet adhesion are the initiating factors of arterial thrombosis. The methods for inducing thrombus formation are mainly physical and chemical. Chemical methods such as trypsin thrombosis method, hydrogen peroxide method, c...

AI Technical Summary

Problems solved by technology

Chemical methods such as trypsin thrombosis method, hydrogen peroxide method, carrageenan, ferric chloride thrombosis method, etc., are limited due to the need to perfuse certain chemical substances. Physical methods such as current injury method require constant current, The blood vessel temperature is not affected by the surrounding tissue, and the arterial short circuit method requires a silicone tube, which has certain limitations

The hanging-wire thrombus model is one of the commonly used models to understand the fate of emboli in the body and evaluate the efficacy of thrombolytic drugs as a whole. It is simple, inexpensive, intuitive and convenient. Reversible, poor stability