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Method for extracting and separating rosmarinic acid and ursolic acid from rosemary

A technology of rosmarinic acid and rosemary, applied in the separation/purification of carboxylic acid esters, steroids, organic chemistry, etc., can solve the problems of low cost, low yield, large active ingredient content, etc., and achieve Low production cost, simple operation, and improved economic efficiency

Inactive Publication Date: 2013-02-20
长沙卫一生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0023] The purpose of the present invention is to provide a method for extracting and separating rosmarinic acid and ursolic acid from rosemary. Content and yield are not high, and a method of using rosemary as raw material and simple process, convenient operation, low cost, high yield and suitable for industrial large-scale extraction and separation of rosmarinic acid and bear from rosemary is proposed. AHA method

Method used

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  • Method for extracting and separating rosmarinic acid and ursolic acid from rosemary
  • Method for extracting and separating rosmarinic acid and ursolic acid from rosemary
  • Method for extracting and separating rosmarinic acid and ursolic acid from rosemary

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Effect test

Embodiment 1

[0048] Weigh 100Kg of rosemary, crush it, place it in 300L (V / V) of 60% ethanol at 40°C for 4 hours in vacuum, filter it once with a ceramic membrane; Filter the membrane again, and combine the two filtrates; add an appropriate amount of antioxidant 150g to the filtrate and concentrate in vacuum at 50°C until 12kg of dry matter is obtained; dissolve the obtained dry matter with 60L of 30% ethanol, filter it once with a ceramic membrane, and put the filtrate on the LSA-10 macroporous resin column, after adsorption, remove impurities with 60L petroleum ether and 60L ethyl acetate respectively, desorb with 60L pH3.5, 45% ethanol solution, collect eluate A; filter residue 8kg 16L of acetone was dissolved, filtered, and the filtrate was applied to an LSA-10 macroporous resin column. After adsorption, impurities were removed with petroleum ether and ethyl acetate, and 40L of pH6.5, 60% methanol solution was used for desorption and collected. Eluate B; Concentrate eluate A and eluate...

Embodiment 2

[0050] Weigh 200Kg of rosemary, crush it, place it in 600L (V / V) of 60% ethanol at 40°C for 4h in vacuum, filter it once with a ceramic membrane; Filter the membrane again and combine the two filtrates; add an appropriate amount of antioxidant 250g to the filtrate and concentrate in vacuum at 50°C until 25kg of dry matter is obtained; dissolve the obtained dry matter with 125L of 30% ethanol, add activated carbon for decolorization for 15min, and then ceramic Membrane filtration once, the filtrate was put on LSA-10 macroporous resin column, after adsorption, impurities were removed with 120L petroleum ether and 120L ethyl acetate respectively, desorbed with 120L pH3.5, 45% ethanol solution, collected and eluted Matter A; filter residue 20kg dissolves with 40L acetone, filters, and the LSA-10 macroporous resin column on the filtrate, after adsorption, carries out impurity removal with sherwood oil, ethyl acetate respectively, uses the pH6.5 of 80L, 60% methyl alcohol The soluti...

Embodiment 3

[0052]Weigh 500Kg of rosemary, pulverize, place 1500L (V / V) in 60% ethanol at 40°C for vacuum insulation for 4h, and filter once with ceramic membrane; filter residue is added to 1000L of 60% ethanol, 40°C for vacuum insulation for 4h, Filtrate the ceramic membrane again, and combine the two filtrates; add an appropriate amount of antioxidant 500g to the filtrate and concentrate in vacuum at 50°C until 70kg of dry matter is obtained; dissolve the obtained dry matter with 350L of 30% ethanol, add activated carbon for decolorization for 15min Filter once with a ceramic membrane, put the filtrate on an LSA-10 macroporous resin column, and after adsorption, remove impurities with 300L petroleum ether and 300L ethyl acetate, desorb with 300L pH3.5, 45% ethanol solution, and collect the washed De-material A; filter residue 45kg was dissolved with 90L of acetone, filtered, and the filtrate was put on LSA-10 macroporous resin column, after adsorption, impurities were removed with petro...

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Abstract

The invention mainly relates to a method for extracting and separating rosmarinic acid and ursolic acid from rosemary. The method comprises the following steps of: grinding the rosemary serving as a raw material, extracting, concentrating, performing column chromatographic separation, extracting, concentrating, crystallizing, recrystallizing, and thus obtaining the rosmarinic acid and the ursolic acid. The whole separation process has low requirements on environmental conditions and equipment, the rosmarinic acid and the ursolic acid have high yield, the shortest separation time, a simple operation method and high purity, and the raw material has the highest comprehensive utilization rate; in addition, the separation materials are readily available and cheap, and the separation operation process is simple and is easy to control; and crystallization and recrystallization are utilized, so that the method is high in purification efficiency and low in cost.

Description

Technical field: [0001] The invention belongs to the field of natural product chemistry, and in particular relates to a method for extracting and separating antioxidants from rosemary, the main active components being rosmarinic acid and ursolic acid. Background technique [0002] Antioxidants are used to prevent or delay the oxidation of animal and vegetable oils, oily foods, instant noodles, meat products and beverages, etc., to prolong the storage period of products, or to achieve the purpose of freshness. Antioxidants are mainly divided into natural antioxidants Two types of researchers, synthetic antioxidants and synthetic antioxidants, have conducted high-dose animal experiments on synthetic antioxidants and natural antioxidants. The results show that synthetic antioxidants can not only significantly cause hepatomegaly, but also increase the activity of liver and lung microsomal enzymes, thereby Turn other substances ingested by the human body into toxic substances or ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/48C07J63/00
Inventor 刘喜荣刘正芳王腊霜
Owner 长沙卫一生物科技有限公司
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