Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting dehydrated vinblastine and epoxy vinblastine from fresh grass of catharanthus roseus

A technology for dehydrating vinblastine and epoxy vinblastine, which is applied in the direction of organic chemistry and the like, can solve the problems of affecting extraction sufficiency, being unsuitable for mass production, and low in safety, and achieves low cost, easy large-scale industrial production, and production technology. simple effect

Active Publication Date: 2014-06-11
上海安体康生植物化学有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, organic solvent extraction is also widely used. Vinca alkaloids are often extracted with organic solvents. After the alkaloids are acidified and alkalized, a large amount of chloroform, benzene, carbon tetrachloride and other solvents are used for extraction, which often brings in a large amount of impurities. And it often forms serious emulsification during extraction, which greatly affects the adequacy of extraction, and is highly toxic and low in safety, so it is not suitable for mass production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 100 kilograms of fresh periwinkle flowers, beat and pulverize them, soak them in 0.5% sulfuric acid solution twice, and soak them for 4 hours each time. Combine the soaking solution, adjust the pH value of the soaking solution to 2, pass through the cation exchange resin HD-8 at 4 times the bed volume / hour, weak acid macroporous type, column diameter ratio 8:1, and wash with water until it is colorless. Soak with 4% ammonium chloride and 3% ethanol solution for 4 hours. Elute with 2% sodium hydroxide and 5% ethanol, detect the appearance of alkaloids by TLC, and start collecting. Combine and concentrate for treatment, extract and alkalize the solution and evaporate to dryness and separate by silica gel chromatography to obtain 40 grams of alkaloids, including 1.14 grams of vinblastine, 0.08 grams of vincristine, 0.65 grams of dehydrated vinblastine, 0.63 grams of epoxy vinblastine, and 0.63 grams of vinblastine. 3.24 grams of alkali and 3.20 grams of Wen Duoling. ...

Embodiment 2

[0027] Take 100 kg of fresh periwinkle flowers, beat and pulverize them, soak and extract them twice with 0.5% sulfuric acid solution. Soak for 4 hours each time. Combine the soaking solution and adjust the pH value of the soaking solution to 3. Pass through cation exchange resin D61, sodium type, column diameter ratio 8:1 at 4 times bed volume / hour, and elute with water until colorless. Soak with 4% sodium chloride and 2% ethanol solution for 4 hours. Elute with 3% sodium hydroxide and 10% ethanol, detect the appearance of alkaloids by TLC, and start collecting. Combine and concentrate for treatment, extract and alkalize the liquid and evaporate to dryness and separate by silica gel chromatography to obtain 40 grams of alkaloids, including 1.15 grams of vinblastine, 0.08 grams of vincristine, 0.68 grams of dehydrated vinblastine, 0.68 grams of epoxy vinblastine, and 0.68 grams of vinblastine. Alkali 3.47 grams, Wen Duoling 3.71 grams.

Embodiment 3

[0029] Take 100 kg of fresh periwinkle flowers, beat and pulverize them, soak and extract them twice with 0.5% sulfuric acid solution. Soak for 6 hours each time. Combine the soaking solution and adjust the pH value of the soaking solution to 4. Pass through cation exchange resin D61 at 4 times bed volume / hour, hydrogen type, column diameter ratio 7:1, eluted with water until colorless. Then soak in 6% ammonium chloride 2% ethanol for 4 hours. Elute with 3% ammonium hydroxide and 22% ethanol, detect the appearance of alkaloids by TLC, and start to collect. Combine and concentrate for treatment, extract and alkalize the solution and evaporate to dryness and separate by silica gel chromatography to obtain 40 grams of alkaloids, including 1.53 grams of vinblastine, 0.09 grams of vincristine, 0.78 grams of dehydrated vinblastine, 0.75 grams of epoxy vinblastine, and 0.75 grams of vinblastine. 3.48 grams of alkali and 3.72 grams of Wen Duolin.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting dehydrated vinblastine and epoxy vinblastine from fresh grass of catharanthus roseus. The method is characterized by comprising the following steps of: 1, cutting and pulping fresh catharanthus roseus, soaking twice in 4 to 8 hours each time by using sulphuric acid liquid, regulating a pH value to be between 1 and 6, filtering, and passing through a macroporous strong acid type cation exchange resin column at a speed of four times bed volume / hour, rinsing to remove impurities, and eluting to a colorless state, wherein the rate of column to diameter is between 7:1 and 8:1; and 2, soaking by using a salt solution, eluting by using a sodium hydroxide solution which has the concentration of 1 to 4 percent and contains ethanol of 2 to 35 percent, detecting an eluent by thin-layer chromatography (TLC), collecting, condensing, drying by distillation, performing chromatographic separation through a silicagel column, and thus obtaining alkaloid, wherein the alkaloid comprises the following components in percent by weight: 2.8 to 4.1 percent of vinblastine, 8.5 to 9.5 percent of vindoline, 0.2 to 0.3 percent of vincristine, 1.3 to 2.0 percent of epoxy vinblastine, 8.1 to 9.1 percent of catharanthine, 1.3 to 2.0 percent of dehydrated vinblastine and the balance of other alkaloids.

Description

technical field [0001] The present invention relates to a technology for extracting effective components of traditional Chinese medicine from periwinkle flowers, in particular to a method for extracting alkaloids from periwinkle flowers. The medicinal active ingredients extracted from periwinkle flowers include dehydrated vinblastine, epoxy vinblastine Alkali, vinblastine, etc., belong to the technical field of natural medicines. Background technique [0002] Vinca is an important medicinal plant in the genus Vinca in the family Apocynaceae. So far, more than 70 alkaloids have been isolated, including Vincristine, Vinblastine, Vindestine, and Wenduolin. (Vindoline) and other ingredients with important medicinal value. Vinblastine is used in the treatment of various solid tumors, and vincristine is mainly used in the treatment of acute leukemia in children. In the 1970s, Eli Lilly researched and developed Vindestine for the treatment of melanoma, prolymphocytic leukemia and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D519/04C07D471/20C07D471/22
Inventor 韩彬倪新忠
Owner 上海安体康生植物化学有限公司