Method for increasing transplanting success rate of tissue culture plantlets of honeysuckle

A technology for tissue culture of seedlings and honeysuckle, applied in the fields of botanical equipment and methods, horticulture, and application, can solve the problems of complicated procedures, inability to meet large-scale production, seasonal restrictions, etc.

Inactive Publication Date: 2012-05-09
重庆市秀山红星中药材开发有限公司
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  • Abstract
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Problems solved by technology

At present, honeysuckle is mainly propagated by traditional methods such as cuttings, sowing, grafting, and layering, which are not only limited by seasons, but also have a low survival rate and cannot meet the needs of large-scale production; although grafting propagation has the advantages of strong plant adaptability and well-developed root system, However, a large number of wild resources must be collected, and the process is complicated, and the survival rate is also very low.
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Method used

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Examples

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with embodiment.

[0017] 1. Take 1,000 tissue-cultured seedlings with 3-4 roots to the greenhouse, harden the seedlings for 3 days, light for 14 hours a day, light intensity is 12,000 Lxu, temperature is 16-28°C, and the air humidity is kept above 80%. , gradually open the cap of the culture bottle.

[0018] 2. Prepare the cultivation substrate according to the ratio of peat: perlite = 4:1. The moisture content of the substrate is 50-60%. When you pinch it with your hands, it will feel moist, and it will remain in agglomerates without dripping out.

[0019] 3. Gently take out the seedlings, 3 plants at a time, carefully wash the root medium with water, repeat 2 times, then soak in the carbendazim liquid with a concentration of 1000-1200 times, gently pick it up after 2 minutes, Gently transplant in the hole tray, the transplanting depth is 1cm, then lightly press the surface of the substrate, and the...

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Abstract

A method for increasing transplanting success rate of tissue culture plantlets of honeysuckle enables honeysuckle plants to gradually adapt to external environment by means of seedling hardening, transplanting, recovery-phase management, growth-phase management and outplanting management, so that the honeysuckle plants correspondingly vary in physiology, form and organization to be more adaptive to the nature, the success rate of the tissue culture plantlets of honeysuckle is increased greatly, and the plants grow healthily and well.

Description

technical field [0001] The invention relates to a plant tissue culture rapid propagation technology, in particular to a method for transplanting honeysuckle tissue culture seedlings. Background technique [0002] Honeysuckle belongs to Lonicera, and the whole plant can be used as medicine. The buds contain volatile aromatic oils, inositol, saponins, chlorogenic acid, flavonoids, cinnamic acid, nerolidol, jasmonal, etc. The stems contain alkaloids. It contains honeysuckle and luteolin, etc. It is one of the traditional Chinese medicinal materials, and it is also a raw material for both medicine and food. It has the effects of clearing away heat and detoxifying, cooling wind and heat, protecting the liver and promoting gallbladder. It has been used in the field of traditional Chinese medicine for carbuncles and boils. , sore throat, erysipelas, heat-toxin bloody dysentery, wind-heat cold, and treatment of warm-heat diseases; now it is more used for health care and health tea, ...

Claims

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Application Information

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IPC IPC(8): A01G1/00
Inventor 何全慧李大根杨再江赵勇彪祝利
Owner 重庆市秀山红星中药材开发有限公司
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