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Method for lily virus-free cultivation through using moroxydine drug

A technology of virus spirit and lily, which is applied in plant tissue culture rapid propagation technology and biological field, can solve the problem of low survival rate of shoot tip culture, and achieve the effect of improving detoxification efficiency, high detoxification efficiency and short time

Inactive Publication Date: 2012-05-09
杨凯 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The survival rate of shoot tip culture is low

Method used

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  • Method for lily virus-free cultivation through using moroxydine drug
  • Method for lily virus-free cultivation through using moroxydine drug
  • Method for lily virus-free cultivation through using moroxydine drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0042] Embodiment: (the virus detection of virus-free tissue culture seedling)

[0043] (1) Plant material

[0044] The tissue culture seedlings of the control group and the experimental group were used as materials.

[0045] (2) Extraction of viral RNA

[0046] Adopt TRIzol reagent, carry out total RNA extraction to each test sample respectively, the preparation method of the RNase-free water used in the extraction process: fill up 0.1% (v / v) DEPC ultrapure water in the clean glass bottle, Autoclave sterilization of glass bottles after overnight treatment; RNase-free plastic and glassware treatment methods: glassware can be baked at 150°C for 6 hours; plastic vessels can be soaked in 0.5M NaOH for 10 minutes, then thoroughly cleaned with DEPC water, and then autoclaved Sterilize. The specific operation steps are as follows:

[0047] 1) The sample to be tested should be placed in a mortar, add a small amount of liquid nitrogen, quickly grind into powder, and add 1ml TRIzol...

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Abstract

The invention discloses a method for lily virus-free cultivation through using moroxydine drug, belonging to the fields of plant culture rapid propagation technique and biotechnology. The method comprises the steps of detecting lily seed balls virus, preparing culture medium, standby, sterilizing and vaccinating explants, culturing the explants and detecting tissue culture seedling virus, etc so as to obtain a large number of virus-free tissue culture seedlings quickly. The best induction culture medium formula that lilies are directly divided into sprouts and root-free tissue culture seedlings is obtained through an orthogonal method; the optimal virus-free concentration is screened through preparing a series of moroxydine antiviral drugs with concentration gradient; the virus-free efficiency can reach 91%; the induction culture period is shortened; the method has the characteristics of high virus-free efficiency and high enhancement factor, etc; and the method offers technological base to culture lily virus-free plants and popularize virus-free cultivation research.

Description

1. Technical field [0001] The invention discloses a method for detoxifying and cultivating lilies by using a virus spirit agent, and belongs to the field of plant tissue culture and rapid propagation technology and biotechnology. 2. Background technology [0002] Lily is the general name of the genus Lilium (Lilium) of the monocot class Liliaceae (Liliaceae), which is a perennial herbaceous flower. There are more than 90 species of Lilium in the world, and about 47 species originate in my country, accounting for 51% of the world total. There are many varieties of lilies, with huge flowers, rich colors, and beautiful flowers. They can be used as cut flowers, potted flowers, and in gardens and green spaces. Most lilies are edible, and they are the best nourishing products. Lily has the meaning of "a hundred years of harmony" and is deeply loved by people. Lilies are mainly distributed in temperate regions such as China, Japan, North America and Europe. In recent years, the p...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12Q1/70C12Q1/68
Inventor 杨凯于同泉杨柳路平路苹王建立
Owner 杨凯
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