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Method for improving compound efficiency of bone morphogenetic protein and true bone ceramic carrier

A technology of morphogenic protein and recombination efficiency, applied in the field of preparation of biomedical materials, can solve problems such as slow process

Inactive Publication Date: 2012-05-30
DALIAN CHUANGDA TECH TRADE MARKET
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of dialysis and liquid replacement is slow, and repeated dialysis is required to completely remove guanidine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The first embodiment of the present invention is the preparation of calcined bone carrier should take fresh pig bone femur rear end, remove muscle and fascia, o. Soak in 5 mol NaoH, bleach with 30% hydrogen peroxide, degrease with 3:1 chloroform and methanol, cut the preform after drying, then place it in a muffle furnace, and heat it slowly to 600 。 C and constant temperature, the organic matter is basically calcined and removed, slowly lowered to normal temperature; then heated to 1100 。 C and calcined at a constant temperature to partially ceramicize the material to increase the strength, slowly lower to room temperature, and cut and grind to shape. Then the composite of calcined bone and bone morphogenetic protein is to dissolve bone morphogenetic protein (BMP) in phosphate buffer solution pH7.2 containing 6 m01 urea, after centrifugation, take the supernatant and soak calcined bone carrier, soak and stir for 24 hour, then add absolute ethanol according to the ...

Embodiment 2

[0018] The first embodiment of the present invention is the preparation of calcined bone carrier should take fresh bovine bone femur rear end, remove muscle and fascia, o. Soak in 5 m01 Na0H, bleach with 30% hydrogen peroxide, degrease with 3:1 chloroform and methanol, cut the preform after drying, put it in the muffle furnace, and heat it slowly to 600 。 C and constant temperature, the organic matter is basically calcined and removed, slowly lowered to normal temperature; then heated to 1100 。 C and calcined at a constant temperature to partially ceramicize the material to increase the strength, slowly lower to room temperature, and cut and grind to shape. Then there is the compounding of calcined bone and bone morphogenetic protein. Bone morphogenetic protein (BMP) is dissolved in phosphate buffer solution pH 7.2 containing 8 mol urea. hour, then add absolute ethanol according to the volume to a final concentration of 85% (V / V), mix well and then store at -70 。 Place C...

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PUM

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Abstract

The invention relates to the field of preparation of biomedical materials, in particular to a method for improving compound efficiency of bone morphogenetic protein and a true bone ceramic carrier, which includes steps of (1) preparation of true bone ceramic and (2) compounding of the true bone ceramic carrier and the bone morphogenetic protein (BMP). By coactions of physical infiltration and deposition, electrical ion adsorption, non-specific chemical adsorption and the like, the method improves compound efficiency of the bone morphogenetic protein (BMP) and the true bone ceramic carrier.

Description

technical field [0001] The invention belongs to the field of preparation of biomedical materials. In particular, it refers to a method for improving the composite efficiency of bone morphogenetic protein and calcined bone carrier. Background technique [0002] Bone morphogenetic protein (BMP) is a body protein factor with a unique function of inducing osteogenesis, which plays a key role in the regeneration and repair of bone tissue in the body. Now it is possible to biochemically extract bone morphogenetic proteins (BMP) from different species from cattle, horses, pigs, sheep, rabbits, mice, and human bone or osteosarcoma, or to prepare genetically recombined human bone morphogenetic proteins using bioengineering techniques. protein (BMP). Utilizing its inductive osteogenic activity, it is expected to realize rapid regenerative connection of human broken bones and rapid regenerative repair of missing bones. It has broad clinical application prospects in orthopedics and st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/42A61L27/54
Inventor 王爽
Owner DALIAN CHUANGDA TECH TRADE MARKET