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Method for preparing fluorescence labeling polyactic acid nanometer microsphere

A nano-microsphere and fluorescent labeling technology, applied in the field of biomedicine, can solve the problems of complicated synthesis process, low product yield and high cost, and achieve the effect of simple process

Inactive Publication Date: 2013-06-12
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the existing technology, not only the synthesis process is cumbersome, the product yield is low, and the cost is high, but also some chemical reagents with serious pollution are used in the process, so the design and synthesis of various high-performance fluorescent labeling nanomaterials have encountered bottlenecks.

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  • Method for preparing fluorescence labeling polyactic acid nanometer microsphere
  • Method for preparing fluorescence labeling polyactic acid nanometer microsphere
  • Method for preparing fluorescence labeling polyactic acid nanometer microsphere

Examples

Experimental program
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Effect test

Embodiment 1

[0030] 1. Preparation of polylactic acid nanoparticles with fluorescent labeling properties:

[0031] (1) Disperse 100 mg of polylactic acid microspheres with an average particle size of 120 nm in 50 ml of distilled water to form a mixture A.

[0032] (2) 1 mg fluorescent dye rhodamine B was dissolved in 1 g glycidyl methacrylate to form a mixed solution B.

[0033] (3) Under magnetic stirring, take 50 mg of mixed solution B and add it to mixed solution A.

[0034] (4) Heat up to 60 °C, add 0.25 mg of ammonium persulfate and sodium ascorbate oxidation-reduction initiator (the mixing mass ratio of ammonium persulfate and sodium ascorbate is 2:3), stop stirring after 6 h, and obtain fluorescently labeled poly Suspension of lactic acid microspheres.

[0035] (5) Put the above suspension in a dialysis bag, and dialyze with redistilled water for 48 hours to remove ungrafted Rhodamine B.

[0036] (6) Freeze-dry the suspension permeated from the dialysis bag to obtain fluorescentl...

Embodiment 2

[0041] 1. Preparation of fluorescently labeled polylactic acid nanospheres loaded with felodipine (an antihypertensive drug):

[0042] (1) Suspend and disperse 50 mg of felodipine-loaded polylactic acid microspheres with an average particle size of 500 nm in 50 ml of distilled water to form a mixture C.

[0043] (2) 1 mg of fluorescent dye rhodamine B was dissolved in 1 g of glycidyl methacrylate to form a mixture D.

[0044] (3) Under magnetic stirring, take 50 mg of mixed solution D and add it to mixed solution C.

[0045] (4) Heat up to 60 °C, add 0.25 mg of ammonium persulfate and sodium ascorbate oxidation-reduction initiator (the mixing mass ratio of ammonium persulfate and sodium ascorbate is 2:3), stop stirring after 6 h, and obtain the drug-loaded fluorescent label Polylactic acid microsphere suspension.

[0046] (5) Take the above suspension and place it in a dialysis bag, and dialyze it with double-distilled water for 48 hours to remove ungrafted rhodamine B.

[...

Embodiment example 3

[0050] 1. Preparation of fluorescently labeled cyclosporin A-loaded polylactic acid nanospheres:

[0051] (1) Disperse 100 mg of cyclosporin A-loaded polylactic acid microspheres with an average particle size of 200 nm in 50 ml of distilled water to form a mixture E.

[0052] (2) 1 mg of the fluorescent dye rhodamine B was dissolved in 1 g of glycidyl methacrylate to form a mixed solution F.

[0053] (3) Under magnetic stirring, take 50 mg of the mixed solution F solution and add it to the mixed solution E.

[0054] (4) Heat up to 60 °C, add 0.25 mg of ammonium persulfate and sodium ascorbate oxidation-reduction initiator (the mixing mass ratio of ammonium persulfate and sodium ascorbate is 2:3), stop stirring after 6 h, and obtain the drug-loaded fluorescent label Suspension of polylactic acid microspheres.

[0055] (5) Put the above suspension in a dialysis bag, and dialyze with distilled water for 48 hours to remove ungrafted Rhodamine B.

[0056] (6) Freeze-dry the susp...

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Abstract

A method for preparing a fluorescence labeling polyactic acid nanometer microsphere relates to the technical field of biological medicine. The method utilizes a functional monomer which can be highly combined with fluorescence compound to perform graft copolymerization on the surface of a polylactic acid (PLA) microsphere so as to carry out fluorescence labeling on the PLA microsphere. The methodfor preparing the fluorescence labeling polyactic acid nanometer microsphere is simple in process, and the prepared nanometer microsphere has stable fluorescence property and good biological compatibility.

Description

technical field [0001] The invention relates to products and methods in the technical field of biomedicine, in particular to fluorescently labeled polylactic acid (PLA) nano-microspheres and a preparation method thereof. Background technique [0002] With the application of modern medicine, molecular biology and various advanced fluorescence detection instruments and technologies, fluorescent labeling, as a non-radioactive labeling technology, has been applied in various research fields of life science and has achieved rapid development. Fluorescent labeling has the characteristics of non-radioactive, easy to operate, high stability, high sensitivity and high selectivity, and there are many kinds of fluorescent labeling dyes and flexible methods, which can be applied to the labeling of various biological macromolecules and drugs. After searching the prior art, it is found that the preparation of nanoparticles with fluorescent labeling properties generally adopts the method o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/14C09K11/06B82Y40/00
Inventor 朱爱萍栗凤娟
Owner YANGZHOU UNIV
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