Lipopetide biosurfactant oil extraction agent for improving extraction rate of crude oil
A technology of surfactants and bio-lipids, applied in the direction of microorganisms, drilling compositions, methods based on microorganisms, etc., can solve the problems of high cost, low recovery rate and serious pollution of chemical oil recovery agents, and achieve excellent performance, Effect of improving rock wettability and reducing crude oil viscosity
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Embodiment 1
[0024] A biological lipopeptide surfactant oil recovery agent for improving crude oil recovery of the present invention: the lipopeptide surfactant-producing bacteria Bacillus subtilis ACCC01430 is fermented to obtain a fermented liquid, and the fermented liquid and the nutrient liquid are divided into 3: After mixing at a volume ratio of 1, add a weak base of sodium carbonate to adjust the pH to 8-9, and then make a biolipopeptide surfactant oil displacement agent;
[0025] The nutrient solution is 1% of molasses, 1% of urea, 0.5% of peptone, 0.2% of yeast extract, 0.3% of diammonium hydrogen phosphate, and the balance is water.
Embodiment 2
[0027] A biological lipopeptide surfactant oil recovery agent for improving crude oil recovery of the present invention: the lipopeptide surfactant-producing bacterium Bacillus subtilis ACCC01430 is fermented to obtain a fermented liquid, and the fermented liquid and the nutrient liquid are mixed according to 5: After mixing at a volume ratio of 1, add a weak base of sodium carbonate to adjust the pH to 8-9, and then make a biolipopeptide surfactant oil displacement agent;
[0028] The nutrient solution is 1% of molasses, 1% of urea, 0.5% of peptone, 0.2% of yeast extract, 0.3% of diammonium hydrogen phosphate, and the balance is water.
Embodiment 3
[0030] A biological lipopeptide surfactant oil recovery agent for improving crude oil recovery of the present invention: the lipopeptide surfactant-producing bacterium Bacillus subtilis ACCC01430 is fermented to obtain a fermented liquid, and the fermented liquid and the nutrient liquid are divided into 4: 1 volume ratio, add weak alkali sodium carbonate to adjust the pH to 8-9, and then make the biological lipopeptide surfactant oil displacing agent;
[0031] The nutrient solution is 1% of molasses, 1% of urea, 0.5% of peptone, 0.2% of yeast extract, 0.3% of diammonium hydrogen phosphate, and the balance is water.
[0032] The title and strain source of the bacterium that the present invention adopts are as follows:
[0033]Bacillus subtilis (ACCC01430), China Agricultural Microbiology Culture Collection Center.
[0034] For the standard of the decline period of the thalline in Examples 1 to 4 of the present invention, see the second edition of "Microbiology Tutorial" edited...
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