Process for preparing barley germ oil and low molecular weight peptide by solid enzymolysis-CO2 (carbon dioxide) supercritical extraction
A technology for small molecular peptides and highland barley germ, which is applied in the field of solid-state enzymatic hydrolysis-CO2 supercritical extraction to produce highland barley germ oil and small molecular peptides, can solve the problems of no intensive processing, poor taste of highland barley germ, etc. Economic and social benefits, the effect of retaining biological activity
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Embodiment 1
[0013] Example 1: The lipoxygenase in the lipoxygenase inactivation is inactivated by heating; the solid-state enzymolysis of the raw material is to mix the enzyme-inactivated highland barley germ powder raw material with water so that the water content is 9%, and then mix it with neutral protease And carry out solid-state enzymolysis, the weight ratio of neutral protease and highland barley germ powder with water is 4:100, the temperature of solid-state enzymolysis is room temperature, and the time of solid-state enzymolysis is 24 hours; CO 2 In the supercritical extraction of germ oil, the extraction pressure is 20MPa, the extraction time is 2 hours, and the oil yield of the obtained highland barley germ oil can reach more than 96%. The small molecular peptide extraction uses water as the solvent to extract the highland barley germ powder residue twice, containing small The liquid of the molecular peptide enters the next process and is ready for use; the drying of the small m...
Embodiment 2
[0014] Example 2: The lipoxygenase in the lipoxygenase inactivation is inactivated by heating; the solid-state enzymatic hydrolysis of the raw material is to mix the enzyme-inactivated highland barley germ powder raw material with water so that the water content is 15%, and then mix it with neutral protease And carry out solid-state enzymolysis, the weight ratio of neutral protease and highland barley germ powder with water is 1:100, the temperature of solid-state enzymolysis is room temperature, and the time of solid-state enzymolysis is 120 hours; CO 2 In the supercritical extraction of germ oil, the supercritical extraction pressure is 40MPa, and the extraction time is 1 hour. The oil yield of the obtained highland barley germ oil can reach more than 96%. The small molecular peptide liquid enters the next process and is ready for use; small molecular peptide drying is to dry the liquid containing small molecular peptide by spray drying to obtain small molecular peptide produ...
Embodiment 3
[0015] Embodiment 3: the lipoxygenase in the lipoxygenase inactivation is inactivated by heating; the raw material solid-state enzymolysis is to mix the highland barley germ powder raw material after the enzyme inactivation with water so that the water content is 12%, and then mix it with neutral protease Uniformly carry out solid-state enzymolysis, the weight ratio of neutral protease and highland barley germ powder with water is 2.5:100, the temperature of solid-state enzymolysis is room temperature, and the time of solid-state enzymolysis is 60 hours; CO 2 In the supercritical extraction, the extraction pressure is 30MPa, the extraction time is 1 hour, and the oil yield of the obtained highland barley germ oil can reach more than 96%. The small molecular peptide extraction uses water as the solvent to extract the highland barley germ powder residue twice, containing small molecular peptides The liquid enters the next process and is ready for use; small molecular peptide dryi...
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