Process for preparing barley germ oil and low molecular weight peptide by solid enzymolysis-CO2 (carbon dioxide) supercritical extraction

A technology for small molecular peptides and highland barley germ, which is applied in the field of solid-state enzymatic hydrolysis-CO2 supercritical extraction to produce highland barley germ oil and small molecular peptides, can solve the problems of no intensive processing, poor taste of highland barley germ, etc. Economic and social benefits, the effect of retaining biological activity

Inactive Publication Date: 2012-06-20
陈元涛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor taste of highland barley germ, it is only u

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: The lipoxygenase in the lipoxygenase inactivation is inactivated by heating; the solid-state enzymolysis of the raw material is to mix the enzyme-inactivated highland barley germ powder raw material with water so that the water content is 9%, and then mix it with neutral protease And carry out solid-state enzymolysis, the weight ratio of neutral protease and highland barley germ powder with water is 4:100, the temperature of solid-state enzymolysis is room temperature, and the time of solid-state enzymolysis is 24 hours; CO 2 In the supercritical extraction of germ oil, the extraction pressure is 20MPa, the extraction time is 2 hours, and the oil yield of the obtained highland barley germ oil can reach more than 96%. The small molecular peptide extraction uses water as the solvent to extract the highland barley germ powder residue twice, containing small The liquid of the molecular peptide enters the next process and is ready for use; the drying of the small m...

Embodiment 2

[0014] Example 2: The lipoxygenase in the lipoxygenase inactivation is inactivated by heating; the solid-state enzymatic hydrolysis of the raw material is to mix the enzyme-inactivated highland barley germ powder raw material with water so that the water content is 15%, and then mix it with neutral protease And carry out solid-state enzymolysis, the weight ratio of neutral protease and highland barley germ powder with water is 1:100, the temperature of solid-state enzymolysis is room temperature, and the time of solid-state enzymolysis is 120 hours; CO 2 In the supercritical extraction of germ oil, the supercritical extraction pressure is 40MPa, and the extraction time is 1 hour. The oil yield of the obtained highland barley germ oil can reach more than 96%. The small molecular peptide liquid enters the next process and is ready for use; small molecular peptide drying is to dry the liquid containing small molecular peptide by spray drying to obtain small molecular peptide produ...

Embodiment 3

[0015] Embodiment 3: the lipoxygenase in the lipoxygenase inactivation is inactivated by heating; the raw material solid-state enzymolysis is to mix the highland barley germ powder raw material after the enzyme inactivation with water so that the water content is 12%, and then mix it with neutral protease Uniformly carry out solid-state enzymolysis, the weight ratio of neutral protease and highland barley germ powder with water is 2.5:100, the temperature of solid-state enzymolysis is room temperature, and the time of solid-state enzymolysis is 60 hours; CO 2 In the supercritical extraction, the extraction pressure is 30MPa, the extraction time is 1 hour, and the oil yield of the obtained highland barley germ oil can reach more than 96%. The small molecular peptide extraction uses water as the solvent to extract the highland barley germ powder residue twice, containing small molecular peptides The liquid enters the next process and is ready for use; small molecular peptide dryi...

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Abstract

The invention provides a process for preparing barley germ oil and a low molecular weight peptide by solid enzymolysis-CO2 supercritical extraction, which belongs to the technical field of barley germ processing, and is characterized in that (1) the process flow comprises five process: lipoxygenase deactivating, raw material solid enzymolysis, CO2 supercritical extraction on the germ oil, extraction on the low molecular weight peptide and drying on the low molecular weight peptide; (2) the process conditions are shown as follows: in the lipoxygenase deactivating, original lipoxygenase in theraw material of barley germ powder is deactivated in the manner of heating or microwaves and the like; in the raw material solid enzymolysis, distributing water to the raw material of the barley germpowder to enable the water content of the raw material of the barley germ powder to reach 9-15 percent, and then, neutral protease is added for the solid enzymolysis; in the CO2 supercritical extraction on the germ oil, the extraction pressure is 20-40MPa, and the extraction time is 1-2 hours; in the extraction on the low molecular weight peptide, the dregs of the barley germ powder are extractedfor 2-3 times by the water to obtain the liquid containing the low molecular weight peptide; and in the drying on the low molecular weight peptide, the liquid containing the low molecular weight peptide is dried in the manner of spray drying or fluidized bed drying to obtain a low molecular weight peptide product. The prepared barley germ oil and the prepared low molecular weight peptide are widely used for industry of foodstuffs, daily chemicals and the like. The barley germ powder is deeply processed by the process for preparing the barley germ oil and the low molecular weight peptide by the solid enzymolysis-CO2 supercritical extraction, i.e. waste is changed into valuable, thereby, the use value and the market value of the barley germ powder are greatly increased.

Description

1. Technical field: [0001] The invention provides a solid enzymolysis-CO 2 The invention relates to a supercritical extraction process for producing highland barley germ oil and small molecule peptides, which belongs to the technical field of highland barley germ processing. 2. Technical background: [0002] Highland barley is a common name for naked barley, which belongs to the grass family and the genus Hordeum in plant classification. Highland barley is the most important cereal crop on the Qinghai-Tibet Plateau. It has the characteristics of early maturity, cold tolerance, barren tolerance, stable yield, and wide adaptability. Highland barley has made outstanding contributions to solving the food and clothing problems of local farmers and herdsmen, but due to its backward processing technology, it is far from playing its due important role in the process of getting rid of poverty. Therefore, it is imminent to develop the processing and comprehensive utilization technol...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/04C11B1/10C12P21/06A23D9/02A23L1/29A23L1/305A23L33/00
CPCY02P20/54
Inventor 陈元涛张炜范燕青郭幼敬王平平蔡林森郭智军池亚玲刘霞徐江波肖江赵文华
Owner 陈元涛
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