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Novel method for constructing Zn2+ ratio detecting system by solubilizing fluorescent probe in micelle

A new method, micellar technology, is applied in the field of constructing Zn2+ ratiometric fluorescence detection system to achieve the effect of improving accuracy and reducing complexity

Inactive Publication Date: 2012-06-20
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(Nanotechnology 2011, 22, 065501.) and the application of a probe molecule to complete the research of the above work has not been reported yet

Method used

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  • Novel method for constructing Zn2+ ratio detecting system by solubilizing fluorescent probe in micelle
  • Novel method for constructing Zn2+ ratio detecting system by solubilizing fluorescent probe in micelle
  • Novel method for constructing Zn2+ ratio detecting system by solubilizing fluorescent probe in micelle

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Experimental program
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Effect test

Embodiment 1

[0018] 8-carboxamidoquinoline derivatives, the structure is shown in the general formula (R 1 = Cl, R 2 =R 3 =H). The concentration of probe molecules is 1.0×10 -5 mol / L SDS (12mmol / L, 10mmol / L Tris-HCl, pH=7.2) solution, add various common metal ions (Na + 、K + , Mg 2+ , Ca 2+ 、Cr 3+ , Fe 3+ 、Co 2+ 、Ni 2+ 、Cu 2+ , Zn 2+ 、Ag + 、Cd 2+ , Hg 2+ , Pb 2+ ), the fluorescence spectrum was measured when the concentration was 5 times of the probe concentration. The maximum emission wavelength of the probe molecule is 405nm when no metal ions are added; Zn 2+ Make the probe molecules appear double fluorescent peaks at 405 and 505nm, and the fluorescent peak at 405nm remains basically unchanged; Ni 2+ 、Cu 2+ The fluorescence of the solution is quenched, while other metal ions have no obvious effect on the fluorescence spectrum of the solution. Where: the ordinate represents the fluorescence intensity, and the abscissa represents the fluorescence wavelength.

Embodiment 2

[0020] 8-carboxamidoquinoline derivatives, the structure is shown in the general formula (R 1 = Cl, R 2 =R 3 =H). The concentration of probe molecules is 1.0×10 -5 mol / L SDS (12mmol / L, 10mmol / L Tris-HCl, pH=7.2) solution, Zn 2+ When the concentration is 0-100 times of the probe concentration, measure its fluorescence spectrum. With Zn 2+ With the addition of , the fluorescence peak intensity of the probe molecules at 405nm remained basically unchanged, but a new fluorescence peak appeared at 505nm, and its intensity gradually increased. Using the fluorescence peak at 405nm as an internal standard, the probe molecule can be used for Zn 2+ ratiometric fluorescence detection. Where: the ordinate represents the fluorescence intensity, and the abscissa represents the fluorescence wavelength.

Embodiment 3

[0022] 8-carboxamidoquinoline derivatives, the structure is shown in the general formula (R 1 = Cl, R 2 =R 3 =H). The concentration of probe molecules is 1.0×10 -5 mol / L SDS (12mmol / L, 10mmol / L Tris-HCl, pH=7.2) solution, add Zn 2+ and other common metal ions (Na + 、K + , Mg 2+ , Ca 2+ 、Cr 3+ , Fe 3+ 、Co 2+ 、Ni 2+ 、Cu 2+ 、Ag + 、Cd 2+ , Hg 2+ , Pb 2+ ), Zn 2+ When it coexists with other metal ions and the concentration is 5 times of the probe concentration, measure its fluorescence spectrum. Most metal ions (Na + 、K + , Mg 2+ , Ca 2+ 、Cr 3+ , Fe 3+ 、Co 2+ 、Ag + 、Cd 2+ , Hg 2+ , Pb 2+) do not interfere with Zn 2+ The fluorescence emission of the complex, the intensity ratio of its double fluorescence peaks and the presence of only Zn in solution 2+ similar when Ni 2+ 、Cu 2+ It binds tightly to the probe molecule and interferes with Zn 2+ detection. Among them: the vertical axis represents the ratio of the double fluorescence peak intensity, the ...

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Abstract

The invention discloses a novel method for constructing a Zn2+ ratio detecting system by solubilizing single probe molecules through surfactant micelle. A fluorescent molecule probe is made of 8-carboxyl acylamino quinoline simultaneously solubilized into sodium lauryl sulfate micelle and to the surface of the micelle, probe molecules positioned on the surface of the micelle indicates identification signals of fluorescent enhancement and wavelength redshift for Zn2+, fluorescent signals of probe molecules positioned inside the micelle are approximately not affected by the Zn2+, and the fluorescent peak is used as an interior label to realize Zn2+ ratio fluorescence detection.

Description

technical field [0001] The present invention relates to a kind of structure Zn that utilizes 8-carboxamido quinoline derivatives through the solubilization of sodium dodecyl sulfate micelles 2+ A new approach to ratiometric fluorescence detection systems. Background technique [0002] At present, research on molecular fluorescence detection mainly focuses on designing and synthesizing different probe molecules, and studying their spectral characteristics in specific detection systems, such as organic solvents, buffer solutions or biological cells. A large number of research results show that it is a difficult task to implement high-sensitivity detection of target species in aqueous solution using only probe molecules, because strong solvation is often not conducive to molecular binding and signal transmission, which reduces the probe Molecular detection performance. In recent years, the use of surfactants to improve the detection performance of probe molecules has made gre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 张宇郭祥峰贾丽华郑丽波王平
Owner QIQIHAR UNIVERSITY
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