Method for measuring cholesterol content in birds' egg and product thereof

A detection method and technology for cholesterol, applied in the detection field, can solve the problems of large amount of solvent, cumbersome processing steps, long sample processing time, etc., and achieve the effect of saving solvent and easy operation.
CN102507791BInactive Publication Date: 2013-04-10JILIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2013-04-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting cholesterol content in a birds' egg and a product thereof, belongs to the technical field of detection, and adopts the normal pressure microwave-assisted backflow saponification extraction-high performance liquid chromatography method. The method comprises the following operations: 1) fully mixing edible parts of the birds' egg or the product thereof, and then adding absolute alcohol, KOH solution and petrolic ether; 2) performing microwave-assisted backflow saponification extraction for the liquid sample, and then cooling the obtained liquid sample with flow water; 3) repeatedly washing the saponification extraction liquid with deionized water to achieve neutrality, and discarding water phase; 4) concentrating organic phase under the vacuumcondition to nearly dry state, and preparing to-be-tested sample in a manner of constant volume with absolute alcohol; 5) and detecting through the high performance liquid chromatography method. The pretreatment method for the sample disclosed by the invention realizes sample saponification and extraction of a targeted object at the same time, and has the characteristics that the efficiency is high, the time is saved, the operation is simple and easy, and the solvent is saved. The detection method has excellent precision and reproducibility, as well as high recovery rate.
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Description

technical field

[0001] The invention belongs to the technical field of detection technology, and relates to a method for detecting cholesterol content in poultry eggs or products thereof, in particular to a pretreatment method for detecting cholesterol content in poultry eggs or products thereof. Background technique

[0002] Cholesterol is also known as cholesterol. A derivative of cyclopentane polyhydrophenanthrene. As a nutrient, cholesterol has important physiological functions. It is an important component of cell membranes and plays an important role in maintaining the normal structure and function of biofilms. It is also an important component of cholic acid, 7-dehydrocholesterol and vitamin D 3 , sex hormones and other important physiologically active substances. However, long-term intake of large amounts may increase the risk of cardiovascular and cerebrovascular diseases such as atherosclerosis, hypertension, and coronary heart disease.

[0003] Poultry eggs hav...

Claims

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