Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for measuring cholesterol content in birds' egg and product thereof

A detection method and technology for cholesterol, applied in the detection field, can solve the problems of large amount of solvent, cumbersome processing steps, long sample processing time, etc., and achieve the effect of saving solvent and easy operation.

Inactive Publication Date: 2013-04-10
JILIN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the process of detecting cholesterol content in poultry eggs or their products, there are disadvantages such as cumbersome sample pretreatment steps, long sample processing time, and large amount of solvents.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring cholesterol content in birds' egg and product thereof
  • Method for measuring cholesterol content in birds' egg and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] After fully mixing fresh egg yolks, accurately weigh 0.5g of the sample into a three-neck round bottom flask, add 30ml of absolute ethanol, 10ml of 30% KOH solution, and 50ml of petroleum ether (boiling range 60°C) into the flask ;

[0023] The three-necked round-bottomed flask was subjected to microwave-assisted reflux saponification and extraction for 2 minutes under the conditions of microwave power of 800w, temperature of 70°C, and normal pressure. Cool to obtain saponification extract;

[0024] Repeatedly washing the saponification extract obtained in the previous step with deionized water until neutral, discarding the aqueous phase;

[0025] Collect the organic phase in a vacuum drying oven, concentrate to near dryness (10 μl to 200 μl) at a vacuum of 0.085 MPa and a temperature of 50° C., dilute to volume with 5 ml of absolute ethanol, and prepare the test sample through a 0.45 μm filter membrane;

[0026] After the samples were processed by the above pretreatm...

Embodiment 2

[0028] After fully mixing the fresh egg yolk, accurately weigh 0.5g of the sample into a three-neck round bottom flask, add 30ml of absolute ethanol, 10ml of 50% KOH solution, and 60ml of petroleum ether (boiling range 60°C) into the flask ;

[0029] The three-necked round-bottomed flask was subjected to microwave-assisted reflux saponification extraction for 5 minutes under the conditions of microwave power of 500w and temperature of 50°C. After the microwave-assisted reflux saponification extraction was completed, the reflux condenser was rinsed with 3ml of absolute ethanol, and the saponification extract was washed with running water. Cool to obtain saponification extract;

[0030] Repeatedly washing the saponification extract obtained in the previous step with deionized water until neutral, discarding the aqueous phase;

[0031] Collect the organic phase in a vacuum drying oven, concentrate to near dryness (10 μl to 200 μl) at a vacuum of 0.075 MPa and a temperature of 60...

Embodiment 3

[0034] After fully mixing fresh egg yolks, accurately weigh 0.5g of the sample into a three-neck round bottom flask, add 20ml of absolute ethanol, 10ml of 60% KOH solution, and 40ml of petroleum ether (boiling range 60°C) into the flask ;

[0035] The three-necked round-bottomed flask was subjected to microwave-assisted reflux saponification and extraction for 7 minutes under the conditions of microwave power of 300w, temperature of 60°C, and normal pressure. The liquid is cooled with running water to obtain a saponification extract;

[0036] Repeatedly washing the saponification extract obtained in the previous step with deionized water until neutral, discarding the aqueous phase;

[0037] Collect the organic phase in a vacuum drying oven, concentrate to nearly dryness (10 μl to 200 μl) at a vacuum of 0.085 MPa and a temperature of 60° C., dilute to volume with 5 ml of absolute ethanol, and prepare the test sample through a 0.45 μm filter membrane;

[0038] After the sample...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting cholesterol content in a birds' egg and a product thereof, belongs to the technical field of detection, and adopts the normal pressure microwave-assisted backflow saponification extraction-high performance liquid chromatography method. The method comprises the following operations: 1) fully mixing edible parts of the birds' egg or the product thereof, and then adding absolute alcohol, KOH solution and petrolic ether; 2) performing microwave-assisted backflow saponification extraction for the liquid sample, and then cooling the obtained liquid sample with flow water; 3) repeatedly washing the saponification extraction liquid with deionized water to achieve neutrality, and discarding water phase; 4) concentrating organic phase under the vacuumcondition to nearly dry state, and preparing to-be-tested sample in a manner of constant volume with absolute alcohol; 5) and detecting through the high performance liquid chromatography method. The pretreatment method for the sample disclosed by the invention realizes sample saponification and extraction of a targeted object at the same time, and has the characteristics that the efficiency is high, the time is saved, the operation is simple and easy, and the solvent is saved. The detection method has excellent precision and reproducibility, as well as high recovery rate.

Description

technical field [0001] The invention belongs to the technical field of detection technology, and relates to a method for detecting cholesterol content in poultry eggs or products thereof, in particular to a pretreatment method for detecting cholesterol content in poultry eggs or products thereof. Background technique [0002] Cholesterol is also known as cholesterol. A derivative of cyclopentane polyhydrophenanthrene. As a nutrient, cholesterol has important physiological functions. It is an important component of cell membranes and plays an important role in maintaining the normal structure and function of biofilms. It is also an important component of cholic acid, 7-dehydrocholesterol and vitamin D 3 , sex hormones and other important physiologically active substances. However, long-term intake of large amounts may increase the risk of cardiovascular and cerebrovascular diseases such as atherosclerosis, hypertension, and coronary heart disease. [0003] Poultry eggs hav...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 刘静波安晓宁宫新统
Owner JILIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com