Method for measuring configuration and morphology of rice seedling root
A technology of rice and morphological structure, applied in the field of studying the root morphology of rice, can solve the problems of difficult observation and measurement, complexity, lack of pertinence, etc., to achieve convenient and rapid observation and measurement, simple, cheap and easy to obtain devices, and improve research efficiency. Effect
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[0035] Four rice varieties were used as test materials. Zhenshan 97B is a widely popularized indica rice variety in China, IRAT109 is a typical upland rice variety, and 313 and 384 are recombinant inbred individuals constructed from Zhenshan 97B and IRAT109 as parents. IRAT109 has excellent drought resistance and is a general material for research on rice water stress resistance.
[0036] The transparent medium is agarose gel from Invitrogen Company, and the mass ratio concentration is 1%. It is directly prepared with tap water, no sterilization is required, and no nutrients are added. After boiling, cool down to about 60°C to pour glue. After the glue filling is completed, remove the excess medium outside the growth box, cover the lid and wait for use. Pick neat and clean seeds and germinate them at 28°C for 48 hours after adding water. Pick plump, white seeds and insert them into the culture medium under the round hole of the lid with the embryo facing down. It is advisa...
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