Method for measuring configuration and morphology of rice seedling root

A technology of rice and morphological structure, applied in the field of studying the root morphology of rice, can solve the problems of difficult observation and measurement, complexity, lack of pertinence, etc., to achieve convenient and rapid observation and measurement, simple, cheap and easy to obtain devices, and improve research efficiency. Effect

Inactive Publication Date: 2012-06-27
SHANGHAI AGROBIOLOGICAL GENE CENT
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, under natural growth conditions, due to the opacity of the soil medium and the complexity of the environmental conditions, it is difficult to observe and measure in situ
So far, there have been reports on the two-dimensional and three-dimensional structure and morphology analysis of roots, but these methods are not very specific, and they are relatively complicated and not suitable for large-scale experiments.

Method used

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  • Method for measuring configuration and morphology of rice seedling root
  • Method for measuring configuration and morphology of rice seedling root
  • Method for measuring configuration and morphology of rice seedling root

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Embodiment

[0035] Four rice varieties were used as test materials. Zhenshan 97B is a widely popularized indica rice variety in China, IRAT109 is a typical upland rice variety, and 313 and 384 are recombinant inbred individuals constructed from Zhenshan 97B and IRAT109 as parents. IRAT109 has excellent drought resistance and is a general material for research on rice water stress resistance.

[0036] The transparent medium is agarose gel from Invitrogen Company, and the mass ratio concentration is 1%. It is directly prepared with tap water, no sterilization is required, and no nutrients are added. After boiling, cool down to about 60°C to pour glue. After the glue filling is completed, remove the excess medium outside the growth box, cover the lid and wait for use. Pick neat and clean seeds and germinate them at 28°C for 48 hours after adding water. Pick plump, white seeds and insert them into the culture medium under the round hole of the lid with the embryo facing down. It is advisa...

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Abstract

The invention provides a simple and convenient two-dimensional rice root system fixation observation and determination method. The method utilizes a set of simple root system-growing culture device to directly acquire the images of the growth condition of the rice root system and observe and determine the morphology and configuration of the rice root system in combination with computer analysis software, and in particular, the method is suitable for the research of rice deep-rootedness. The method has the technical effect that the method has important reference and application value in rice variety screening, the research of stress resistance and rice breeding.

Description

technical field [0001] The invention relates to a method for studying the morphology of rice roots, in particular to a method capable of simply observing and measuring the configuration and morphology of rice roots at the seedling stage. Background technique [0002] Rice is one of the most important food crops in the world, and it is a hot crop in agricultural biology research. The root system is the main organ for plants to absorb nutrients and water. Different root shapes directly affect the ability of plants to absorb water and nutrients and cope with environmental stress, which will cause significant differences in plant growth and reproduction. The root system of rice belongs to the fibrous root system, which has deep root type and shallow root type. Most of the deep root type varieties have root systems exceeding 20 cm, which can absorb the water in the deep soil; most of the shallow root type varieties are concentrated on the surface of the soil to absorb water from ...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G31/06
CPCY02P60/21
Inventor 楼巧君陈亮梅捍卫夏辉罗利军
Owner SHANGHAI AGROBIOLOGICAL GENE CENT
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