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Citric acid fermented stock solution and preparation method for citric acid and starch sugar

A technology of citric acid and fermentation bacterial agent, applied in the field of fermentation engineering, can solve the problem of low conversion rate of citric acid

Inactive Publication Date: 2012-06-27
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But, experiment proves, the conversion rate of existing citric acid preparation method is still not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0038]The present invention also provides a method for preparing citric acid. The method for preparing citric acid comprises: inoculating a citric acid fermentation agent in the culture medium for fermentation to obtain a fermentation liquid, and then extracting citric acid from the fermentation liquid. The base is the fermentation stock solution as described above.

[0039] Wherein, the method of inoculation and the method of fermentation are conventional methods in the art, and the present invention has no special requirements, for example, the inoculation is carried out by mixing the fermentation stock solution and the citric acid fermentation agent; another example The fermentation can be carried out by a submerged liquid fermentation method. Specifically, the fermentation conditions can include: a temperature of 30-40°C, more preferably 34-38°C; a time of 40-70 hours, more preferably 50-65 hours .

[0040] Wherein, the citric acid fermenting bacteria agent may be a mater...

preparation example 1

[0052] This preparation illustrates the source of the sugar residue.

[0053] 100 parts by weight of corn and 300 parts by weight of 0.28% by weight sulfurous acid aqueous solution are pumped into the soaking tank by a delivery pump. After soaking for 60 hours at a temperature of 50° C., the moisture content of the wet corn after soaking is 43% by weight.

[0054] The soaked corn is broken into 6-12 petals / corn with a convex tooth mill, and the corn germ is freed at the same time, and the germ is separated by a germ cyclone to obtain a material after degermination. Sieve the material after degerming and finely grind the sieved material obtained by sieving, then wash it with water in the fiber washing tank, leave the fiber in the fiber washing tank, combine the sieved material obtained by sieving and wash it with water The material to obtain the fiber-removed product.

[0055] Centrifuge the defibrated product at a speed of 3000g for 20 minutes to obtain the protein layer and...

preparation example 2

[0060] This preparation is used to illustrate the source of the liquefied supernatant.

[0061] The dried corn seeds are pulverized into corn flour that can pass through a 40-mesh mesh through a grinder (Jiangsu Muyang Group Co., Ltd., 968-3 type).

[0062] At room temperature of 25°C, the pulverized product was slurried with water to a concentration of 12 Baume, and the pH value was adjusted to 6.0 to obtain starch milk, and then amylase (Novozymes, α-amylase) was added to the starch milk. , relative to every gram of starch used to produce the starch milk, the amount of amylase is 25 enzyme activity units). Rapidly raise the temperature to 95°C, maintain it for 140 minutes, and then use a filter press to filter the filtrate, which is the liquefied supernatant.

[0063] According to the method specified in the national standard GB 6194-86, the total sugar content in the liquefied supernatant was measured to be 25% by weight.

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Abstract

The invention provides a citric acid fermented stock solution. The citric acid fermented stock solution contains a nitrogen source, sugar scum and a liquefied clear solution, wherein the sugar scum is a filter residue obtained by filtering a saccharifying solution; the saccharifying solution is a product obtained by contacting a liquefied solution of a starchy raw material with a saccharifying enzyme under a saccharifying condition; the liquefied clear solution is a filtrate obtained by filtering the liquefied solution of the starchy raw material; and the starchy raw material used for preparing the saccharifying solution and the starchy raw material used for preparing the liquefied solution are the same or different. The invention also provides a preparation method for a citric acid. The invention also provides a co-production method for the citric acid and starch sugar. Through the technical scheme, the preparation method for the citric acid is capable of reaching conversion rate of over 90 percent.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a citric acid fermentation stock solution, a citric acid preparation method and a co-production method of citric acid and starch sugar. Background technique [0002] Citric acid is an organic acid widely used in industries such as beverage, food and medicine. The preparation method of citric acid mainly includes: preparing starchy raw materials (such as corn, rice and potatoes, etc.) citric acid. [0003] In the existing citric acid preparation method, the fermentation raw liquid generally uses liquefied supernatant liquid and nitrogen source (such as urea and / or ammonium salt) as main components. [0004] However, experiments have shown that the conversion rate of the existing citric acid preparation method is still not high. Contents of the invention [0005] The purpose of the invention is to overcome the defect that the conversion rate of the existing citric acid ...

Claims

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Application Information

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IPC IPC(8): C12P7/48C12P19/14C12R1/685
Inventor 熊结青卢宗梅邵引刚钟华马静
Owner COFCO BIOTECHNOLOGY CO LTD
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