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Method for improving enzymolysis efficiency of lignocellulose by compounding lignocellulose with various materials

A technology of lignocellulose and cellulase, applied in the field of enzymatic hydrolysis of lignocellulose, can solve the problems of low concentration of fermentable sugar, high cost of cellulase, and low efficiency of enzymatic hydrolysis

Inactive Publication Date: 2012-06-27
JILIN UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0006] The invention provides a method for improving the efficiency of enzymatic hydrolysis of lignocellulose, which solves the problems of low enzymatic hydrolysis efficiency, low concentration of fermentable sugar, and high cost of cellulase in the existing enzymatic hydrolysis process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 16.54g of air-exploded straw, add 2ml of citric acid buffer solution with pH4.8, and replenish water to 40g; add 50μl of 10% (v / v) Tween-80 and 100μl of 1% (w / v) bovine serum albumin: After reacting for 1.5 hours, add 2IU / g cellulose β-glucosidase, 36IU / g cellulose xylanase, 0.124IU / g cellulose pectinase, 10% (w / v) thiosulfuric acid Cellulase with sodium 300 μl and 15 FPU / g cellulose, enzymatic hydrolysis at 50°C, 48 hours.

[0019] After the reaction, the supernatant was taken by centrifugation, and the glucose content was measured with a glucose kit, and the glucose conversion rate increase was 40%.

Embodiment 2

[0021] Take 16.54g of air-exploded straw, add 2ml of citric acid buffer solution with pH4.8, and replenish water to 40g; add 50μl of 10% (v / v) Tween-80 and 100μl of 1% (w / v) bovine serum albumin: After reacting for 1.5 hours, add 4IU / g cellulose β-glucosidase, 45IU / g cellulose xylanase, 0.124IU / g cellulose pectinase, 10% (w / v) thiosulfuric acid Cellulase with sodium 300 μl and 15 FPU / g cellulose, enzymatic hydrolysis at 50°C, 48 hours.

[0022] After the reaction, the supernatant was taken by centrifugation, and the glucose content was measured with a glucose kit, and the glucose conversion rate increase was 42%.

Embodiment 3

[0024] Take 16.54g of air-exploded straw, add 2ml of citric acid buffer solution with pH4.8, and replenish water to 40g; add 50μl of 10% (v / v) Tween-80 and 100μl of 1% (w / v) bovine serum albumin: After reacting for 1.5 hours, add 5IU / g cellulose β-glucosidase, 45IU / g cellulose xylanase, 0.108IU / g cellulose pectinase, 10% (w / v) thiosulfuric acid Cellulase with sodium 400 μl and 15 FPU / g cellulose, enzymatic hydrolysis at 50°C for 48 hours.

[0025] After the reaction, the supernatant was taken by centrifugation, and the glucose content was measured with a glucose kit, and the glucose conversion rate increase was 41%.

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Abstract

The invention provides a method for improving the enzymolysis efficiency of lignocellulose, which solves the problems of low enzymolysis efficiency and fermentable sugar concentration and high cellulase cost of the conventional enzymolysis process by improving the enzymolysis efficiency of lignocellulose by adopting various materials. The glucose conversion rate of the lignocellulose after enzymolysis is improved by 46 percent, which provides technical support for producing fuel ethanol with lignocellulose.

Description

technical field [0001] The invention provides a method for compounding cellulase to improve its degrading efficiency of lignocellulose, which uses multiple substances to cooperate with cellulase to improve the enzymatic hydrolysis efficiency of gas-exploded straw, and belongs to the technical field of lignocellulose enzymatic hydrolysis. Background technique [0002] At present, the possibility of using plant fiber materials to produce fuel ethanol has long attracted the attention of scientists and governments all over the world. Some experimental institutions in Europe and the United States have conducted research on this for many years. The development of lignocellulose to produce fuel ethanol is the need to create a new way to develop green renewable energy and reduce harmful gas emissions to protect the environment. [0003] In common crops, cellulose accounts for about 45% of its dry weight. Cellulose molecule is a chain-like high molecular polymer composed of glucose ...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P7/10
CPCY02E50/16Y02E50/10
Inventor 滕利荣任晓冬于潇潇高朝辉张小希邓红波孟庆繁逯家辉刘艳
Owner JILIN UNIV
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