Method of making special cigarette flavors with tobacco stems
A technology for tobacco flavors and tobacco stems, which is applied in the field of microbial dual-strain compound fermentation technology to produce characteristic tobacco flavors, can solve problems such as no reports yet, and achieve the effects of relatively low cost of raw materials, improved richness, and easy process.
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Embodiment 1
[0026] Primary fermentation steps——
[0027] 1. Seed solution preparation: get the Shilin tobacco leaf bacteria obtained by conventional microbial separation and purification technology means to preserve species, and inoculate in the primary seed culture solution (LB liquid medium) by bacteria: seed culture solution=1: 100 (W / W) , sterilized at 121°C for 30min), placed in a shaker (30°C-40°C, 150r / min) for 15h, and obtained the primary seed liquid.
[0028] 2. Raw material treatment: select the raw material of Shilin tobacco stems and pulverize them, add the solvent according to Shilin tobacco stems raw materials: water = 1:10 (W / W), pack and sterilize (121°C, 30min) to obtain aseptic tobacco stem extract.
[0029] 3. Fermentation: inoculate the first-grade seed liquid cultivated in the aseptic tobacco stem extract, and the inoculation ratio is first-grade seed liquid: sterile tobacco stem extract=1:25 (W / W), so that the first-grade seed The liquid and the tobacco stem extrac...
Embodiment 2
[0037] Primary fermentation steps——
[0038] 1. Seed solution preparation: get the Dali tobacco leaf bacteria obtained by conventional microbial separation and purification technology means to preserve species, and inoculate in the first-level seed culture solution (LB liquid medium) by bacteria: seed culture solution=1: 100 (W / W) , sterilized at 121°C for 30min), placed in a shaker (30°C-40°C, 150r / min) for 20h, and obtained the first-grade seed liquid.
[0039] 2. Raw material processing: select the raw material of Shilin tobacco stems and pulverize them, add the solvent according to Shilin tobacco stems raw materials: water = 1:20 (W / W), pack and sterilize (121°C, 30min) to obtain aseptic tobacco stem extract.
[0040] 3. Fermentation: inoculate a cultivated seed liquid into the sterile tobacco stem extract, the inoculation ratio is first-grade seed liquid: sterile tobacco stem extract=1:50 (W / W), so that the first-grade seed liquid Mix well with tobacco stem extract. The...
Embodiment 3
[0048] Primary fermentation steps——
[0049] 1, seed solution preparation: get the Yuxi tobacco leaf bacterium that obtains by conventional microorganism separation and purification technical means to preserve species, press bacterium: seed culture solution=1: 1000 (W / W) inoculate in first-class seed culture solution (LB liquid culture medium , sterilized at 121°C for 30min), placed in a shaker (30°C-40°C, 150r / min) for 24h, and obtained the first-grade seed liquid.
[0050] 2. Raw material processing: select the raw material of Shilin tobacco stems and pulverize them, add a solvent according to Shilin tobacco stems raw materials: water = 1:, 15 (W / W), pack and sterilize (121° C., 30 min) to obtain aseptic tobacco stem extracts.
[0051] 3. Fermentation: Inoculate the cultivated seed liquid into the sterile tobacco stem extract, the inoculation ratio is first-grade seed liquid: sterile tobacco stem extract=1:100 (W / W), so that the first-grade seed liquid Mix well with tobacco...
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